This document specifies a procedure for determining hydroxyacyl-coenzyme A dehydrogenase (HADH) activity in poultry meat samples by spectrophotometry. The results provide an indication of whether poultry breast meat has been previously frozen, based on the ratio of relative HADH activity, and can be used to verify the labelling of poultry breast meat sold as chilled poultry.
This document is applicable specifically to chicken breast meat. It is not applicable to other cuts and/or types of meat unless these have been validated and appropriate limits have been established.
When following the procedure outlined in this document, for a single sample pressing results in 96,6 % of samples being correctly classified. If a correct classification rate of 99,9 % is required, three sample pressings are necessary; i.e. three chicken breasts (one pressing from each), selected randomly from a batch.
This document is not applicable to minced meat or to poultry preparations.

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This document provides a common language covering NTTM in food and feed authentication. It provides:
—   definitions of terms involved in the development and validation of NTTM;
—   a general structure and guidelines for development of NTTM;
—   general considerations for the validation of NTTM.
NOTE   “Food and feed” is implied whenever the term “food” is used in this document.

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This document specifies a rapid method for the determination of the oil and water contents in oilseeds using pulsed nuclear magnetic resonance (NMR). It is applicable to rapeseeds, linseeds and sunflower seeds with a water content less than 10 % and soya beans with a water content less than 14 %. For seeds with higher water contents, drying is necessary before the water and oil content can be determined by pulsed NMR. NOTE 1 This method has been tested with rapeseeds, soya beans, linseeds and sunflower seeds. This does not, however, preclude its applicability to other commercial seeds whose oil is liquid at the temperature of measurement. NOTE 2 The reproducibility values obtained are generally higher than those obtained by the reference methods (see ISO 659 and ISO 665) because they depend on the variability between the instruments and on that existing between the calibrations, which depends on the measurement accuracy of the reference methods. NOTE 3 The results of the comparison test between different magnets and different volumes of seed samples (see Annex B) are in coherence with the results of the interlaboratory test (see Annex A).

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This document gives the botanical names of the main species of oleaginous plants, together with the names of the corresponding raw materials and oils (fats). This document is applicable to both edible oil and non-edible oils. An alphabetical index of the raw materials is also given to assist in the use of this document.

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This document specifies a procedure for determining hydroxyacyl-coenzyme A dehydrogenase (HADH) activity in poultry meat samples by spectrophotometry. The results provide an indication of whether poultry breast meat has been previously frozen, based on the ratio of relative HADH activity, and can be used to verify the labelling of poultry breast meat sold as chilled poultry.
This document is applicable specifically to chicken breast meat. It is not applicable to other cuts and/or types of meat unless these have been validated and appropriate limits have been established.
When following the procedure outlined in this document, for a single sample pressing results in 96,6 % of samples being correctly classified. If a correct classification rate of 99,9 % is required, three sample pressings are necessary; i.e. three chicken breasts (one pressing from each), selected randomly from a batch.
This document is not applicable to minced meat or to poultry preparations.

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This document specifies a spectrophotometric method for the determination of the hydroxypropyl group content of modified starches.

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This document specifies the maximum percentage content of alloying elements and impurities present in wrought aluminium and aluminium alloys which are fabricated into materials and articles designed to be in contact with foodstuff. It contains provisions for the demonstration of conformity of products with the present standard.
NOTE    Materials include semi-finished products. Articles are finished goods.

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This document specifies a method for determining the content of wet gluten and the gluten index for wheat flours (Triticum aestivum L. and Triticum durum Desf.) by mechanical means.
This document is directly applicable to flours. It is also applicable to common and durum wheat after grinding, if their particular size distribution meets the specification given in Table B.1.
NOTE            This document is related to ICC 137/1[7], ICC 155[8] and AACC Method 38-12.02[9].

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This document specifies procedures for the detection of a DNA sequence of a construct used to (genetically) enhance the growth of fish commonly found in aquaculture. The genetically modified AquAdvantage Atlantic salmon (Salmo salar) carries the construct expressing CS-GHc2 growth hormone and can be detected based on a real-time polymerase chain reaction (PCR) targeting either the border between the growth hormone coding sequence (CS-GHc2) of Oncorhynchus tshawytscha (Chinook salmon) and the antifreeze terminator (T-AFP) of (Macro-) Zoarces americanus (ocean pout), i.e. with the construct-specific method, or the border between the Atlantic salmon genomic DNA and the antifreeze promoter (P-AFP) of ocean pout, i.e. with the event-specific method. These methods can be applied to identify the genetically modified (GM) fish or for screening purposes.
This document is applicable for the analysis of DNA extracted from foodstuffs. It can also be suitable for the analysis of DNA extracted from other products such as feedstuffs. The application of these methods requires the extraction of an adequate amount of amplifiable DNA from the relevant matrix.

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This document specifies the maximum percentage content of alloying elements and impurities present in wrought aluminium and aluminium alloys which are fabricated into materials and articles designed to be in contact with foodstuff. It contains provisions for the demonstration of conformity of products with the present standard.
NOTE    Materials include semi-finished products. Articles are finished goods.

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This document specifies a liquid chromatography tandem mass spectrometry method (LC-MS/MS) for the determination of nitroimidazole residues in eggs and egg products. This document is applicable to eggs and egg products, including whole eggs (fresh, frozen or pasteurized), egg yolk (liquid, frozen, dried or pasteurized), egg white (liquid, frozen, dried or pasteurized), whole egg powder, egg white powder, egg yolk powder, and liquid or dried albumin preparations.

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This document specifies the requirements for dehydrated tarragon (Artemisia dracunculus Linnaeus) (methylchavicol type; also called “estragole -type tarragon”) in the form of whole or cut leaves and powder. It does not apply to the elemicin-sabinene-type tarragon. Recommendations relating to storage and transport conditions are given in Annex A.

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This document specifies a procedure for determining whether a perceptible sensory difference or similarity exists between samples of two products. The method is a forced-choice procedure. The method is applicable whether a difference exists in a single sensory attribute or in several attributes.
The method is statistically less efficient than the triangle test (described in ISO 4120) but is easier to perform by the assessors.
The method is applicable even when the nature of the difference is unknown (i.e. it determines neither the size nor the direction of difference between samples, nor is there any indication of the attribute(s) responsible for the difference). The method is applicable only if the products are fairly homogeneous.
The method is effective for:
determining that either:
a perceptible difference results (duo-trio testing for difference); or
a meaningful perceptible difference does not result (duo-trio testing for similarity) when, for example, a change is made in ingredients, processing, packaging, handling or storage;
selecting, training and monitoring assessors.
Two forms of the method are described:
the constant-reference technique, used when one product is familiar to the assessors (e.g. a sample from regular production);
the balanced-reference technique, used when one product is not more familiar than the other.

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This document specifies the maximum mass content of alloying elements and impurities in aluminium and aluminium alloy cast materials and articles designed to be in contact with foodstuff. It contains provisions for the demonstration of conformity of products with the present document.
NOTE   Materials include ingots and liquid metal. Articles are finished goods.

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2012-08-27 GVN: Text for FV received at ISO/CS (see notification of 2012-08-27 in dataservice)
2011-12-06 EMA: Draft for //ENQ received in ISO/CS (see notification of 2011-12-02 in dataservice).
2010-03-03 EMA: WI updated as ISO decided to proceed with an amendment instead of a revision (former ID 53575).

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ISO 21569:2005 describes the procedure to qualitatively detect genetically modified organisms (GMOs) and derived products by analysing the nucleic acids extracted from the sample under study. The main focus is on polymerase chain reaction (PCR) based amplification methods.
It gives general requirements for the specific detection and identification of target nucleic acid sequences (DNA) and for the confirmation of the identity of the amplified DNA sequence.
Guidelines, minimum requirements and performance criteria laid down in ISO 21569:2005 are intended to ensure that comparable, accurate and reproducible results are obtained in different laboratories.
ISO 21569:2005 has been established for food matrices, but could also be applied to other matrices (e.g. feed and plant samples from the environment).
Specific examples of methods are provided in Annexes A to D.

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This document specifies the maximum mass content of alloying elements and impurities in aluminium and aluminium alloy cast materials and articles designed to be in contact with foodstuff. It contains provisions for the demonstration of conformity of products with the present document.
NOTE   Materials include ingots and liquid metal. Articles are finished goods.

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This document specifies procedures for the detection of a DNA sequence of a construct used to (genetically) enhance the growth of fish commonly found in aquaculture. The genetically modified AquAdvantage Atlantic salmon (Salmo salar) carries the construct expressing CS-GHc2 growth hormone and can be detected based on a real-time polymerase chain reaction (PCR) targeting either the border between the growth hormone coding sequence (CS-GHc2) of Oncorhynchus tshawytscha (Chinook salmon) and the antifreeze terminator (T-AFP) of (Macro-) Zoarces americanus (ocean pout), i.e. with the construct-specific method, or the border between the Atlantic salmon genomic DNA and the antifreeze promoter (P-AFP) of ocean pout, i.e. with the event-specific method. These methods can be applied to identify the genetically modified (GM) fish or for screening purposes.
This document is applicable for the analysis of DNA extracted from foodstuffs. It can also be suitable for the analysis of DNA extracted from other products such as feedstuffs. The application of these methods requires the extraction of an adequate amount of amplifiable DNA from the relevant matrix.

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This document specifies a method for determining the content of wet gluten and the gluten index for wheat flours (Triticum aestivum L. and Triticum durum Desf.) by mechanical means.
This document is directly applicable to flours. It is also applicable to common and durum wheat after grinding, if their particular size distribution meets the specification given in Table B.1.
NOTE            This document is related to ICC 137/1[7], ICC 155[8] and AACC Method 38-12.02[9].

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This document specifies the definition and technical criteria for whole grain as a food ingredient (or “raw material”) and further specifies the requirements for designating whole-grain foods derived from cereals and certain pseudocereals on packaging, labelling and claims. The document is applicable to business-to-business and business-to-consumer communication, to the food trade and to food labelling and claims. The definition and technical criteria are only applicable to post-harvest/collecting of the grain. The document specifies technical criteria to all food and beverage companies, applicable to all organizations, regardless of size or complexity. This document is intended for use in business-to-business and business-to-consumer communications, relationships in the global supply chain, the international trade of food products, and food labelling and claims. NOTE The definition, technical criteria and criteria for food labelling and claims are generic. This document does not specify detailed criteria or technical guidance for specific types of grains and products.

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This document specifies a method for determining the content of wet gluten and the gluten index for wheat flours (Triticum aestivum L. and Triticum durum Desf.) by mechanical means. This document is directly applicable to flours. It is also applicable to common and durum wheat after grinding, if their particular size distribution meets the specification given in Table B.1. NOTE This document is related to ICC 137/1[7], ICC 155[8] and AACC Method 38-12.02[9].

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This document specifies a procedure for determining whether a perceptible sensory difference or similarity exists between samples of two products. The method is a forced-choice procedure. The method is applicable whether a difference exists in a single sensory attribute or in several attributes.
The method is statistically less efficient than the triangle test (described in ISO 4120) but is easier to perform by the assessors.
The method is applicable even when the nature of the difference is unknown (i.e. it determines neither the size nor the direction of difference between samples, nor is there any indication of the attribute(s) responsible for the difference). The method is applicable only if the products are fairly homogeneous.
The method is effective for:
determining that either:
a perceptible difference results (duo-trio testing for difference); or
a meaningful perceptible difference does not result (duo-trio testing for similarity) when, for example, a change is made in ingredients, processing, packaging, handling or storage;
selecting, training and monitoring assessors.
Two forms of the method are described:
the constant-reference technique, used when one product is familiar to the assessors (e.g. a sample from regular production);
the balanced-reference technique, used when one product is not more familiar than the other.

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This document specifies procedures for the detection of a DNA sequence of a construct used to (genetically) enhance the growth of fish commonly found in aquaculture. The genetically modified AquAdvantage Atlantic salmon (Salmo salar) carries the construct expressing CS-GHc2 growth hormone and can be detected based on a real-time polymerase chain reaction (PCR) targeting either the border between the growth hormone coding sequence (CS-GHc2) of Oncorhynchus tshawytscha (Chinook salmon) and the antifreeze terminator (T-AFP) of (Macro-) Zoarces americanus (ocean pout), i.e. with the construct-specific method, or the border between the Atlantic salmon genomic DNA and the antifreeze promoter (P-AFP) of ocean pout, i.e. with the event-specific method. These methods can be applied to identify the genetically modified (GM) fish or for screening purposes. This document is applicable for the analysis of DNA extracted from foodstuffs. It can also be suitable for the analysis of DNA extracted from other products such as feedstuffs. The application of these methods requires the extraction of an adequate amount of amplifiable DNA from the relevant matrix.

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This document specifies a method of preparing a ground sample of spice or condiment for analysis, from a laboratory sample obtained by the method specified in ISO 948.
This document is applicable to the majority of spices and condiments. However, in view of the large number and diversity of spices and condiments, it can be necessary in certain special cases, for example, considerable hardness, or high moisture, volatile oil or fat content, to use a modified procedure or to choose another more
suitable method. Any such modified procedure or alternative method will be indicated in the International Standard appropriate to the spice or condiment concerned.

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This document specifies a procedure to determine the release of metals from metallic materials used in products intended to come into contact with drinking water.
The test can be used for three purposes:
a)   To assess a material as a reference material for a new category of materials by metal release testing using the results of several investigations in different waters covering a broad range of water compositions;
b)   To assess a material for an existing category for approval by way of metal release testing using the water defined in part 2, which exhibited the highest metal release when the reference material of the category was tested;
c)   To obtain data on the interaction of local water with a material.

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This document specifies a procedure for obtaining a migration water to determine odour, flavour, colour and turbidity for products made from organic materials intended to come in contact with water for human consumption (drinking water) and used in piping and storage systems. Such products include pipes, tanks, reservoirs, fittings, ancillaries and their coatings both for site applied and factory-made products.
This document is applicable to products to be used under various conditions for the transport, storage and distribution of water intended for human consumption and raw water used for the manufacture of water intended for human consumption.
This document specifies a test method comprising a set of procedures. The use may be dependent on the relevant national regulations and/or the Commission Implementing Decision (EU) 2024/368.

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This document specifies a liquid chromatography tandem mass spectrometry (LC-MS/MS) method for the determination of marker residues of nicarbazin (4,4-dinitrocarbanilide) in chicken tissue and eggs. This document is applicable to the determination of marker residues of nicarbazin (4,4-dinitrocarbanilide) in chicken tissue (including muscle, liver and kidney) and eggs.

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This document specifies the determination of fluoroquinolone residue content in meat, fish and their products by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method. This document is applicable to the determination of enrofloxacin, ciprofloxacin, norfloxacin, ofloxacin and pefloxacin residues in meat, fish and their products, including livestock and poultry.

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This document specifies a procedure for determining whether a perceptible sensory difference or similarity exists between samples of two products. The method is a forced-choice procedure. The method is applicable whether a difference exists in a single sensory attribute or in several attributes. The method is statistically less efficient than the triangle test (described in ISO 4120) but is easier to perform by the assessors. The method is applicable even when the nature of the difference is unknown (i.e. it determines neither the size nor the direction of difference between samples, nor is there any indication of the attribute(s) responsible for the difference). The method is applicable only if the products are fairly homogeneous. The method is effective for: determining that either: a perceptible difference results (duo-trio testing for difference); or a meaningful perceptible difference does not result (duo-trio testing for similarity) when, for example, a change is made in ingredients, processing, packaging, handling or storage; selecting, training and monitoring assessors. Two forms of the method are described: the constant-reference technique, used when one product is familiar to the assessors (e.g. a sample from regular production); the balanced-reference technique, used when one product is not more familiar than the other.

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This document specifies methods for the enumeration of thermoresistant spores of thermophilic bacteria in heat-processed milk and dried milk products by using a colony-count technique at 55 °C after heating the sample at 106 °C or 100 °C. The applicability of this document is limited to heat-processed milk including pasteurized, ultra-high temperature (UHT) processed and sterilized milk; as well as dried whole milk, skim milk, buttermilk and whey products.

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This document specifies the definitions of Zhacai (pickled and squeezed vegetables) and its requirements, including sensory, physical and chemical, contaminants, microorganisms, packaging, labelling, storage and transport. It also describes the corresponding test methods. This document is only applicable to Zhacai.

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This document specifies the requirements for pimento or allspice [Pimenta dioica (L.) Merr.], whole or ground. Recommendations relating to storage and transport conditions are given in Annex A.

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Basis for this method is the laboratory sample obtained by the method specified in ISO 948. The principle of determination consists in grinding the laboratory sample, which has been previously mixed, to obtain particles of the size specified in the International Standard appropriate to the spice or condiment concerned or, if not so specified, to obtain particles of size approximately 1 mm.

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This document specifies requirements for white pepper (Piper nigrum L.) (see ISO 676), whole or ground, at the following commercial stages: semi-processed white pepper (SP); processed white pepper (P). This document is not applicable to white pepper categories called “light”. NOTE Specifications for white pepper are given in ISO 959-1. Recommendations relating to storage and transport conditions are given in Annex B.

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This document specifies requirements for black pepper (Piper nigrum L.) (see ISO 676), whole or ground at the following commercial stages: non-processed black pepper (NP) or semi-processed black pepper (SP); processed black pepper (P), which can, in certain cases, be re-sold directly to the consumers. This document does not apply to black pepper categories called “light”. NOTE Specifications for white pepper are given in ISO 959-2. Recommendations relating to storage and transport conditions are given in Annex C. Information regarding the microscopic structure of the pepper berry is given in Annex D.

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This document specifies a test method for estimating the transfer of mineral oil saturated hydrocarbons (MOSH) and mineral oil aromatic hydrocarbons (MOAH) from food contact materials containing recycled pulp.
This test method is applicable for examining the extent of migration from paper and board equipped with a barrier or other technical solutions to reduce the amount of migration.
This test method is also applicable to paper and board made from virgin fibres.

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This document specifies a method [1] for the quantitative determination of saxitoxin (STX), decarbamoyl saxitoxin (dcSTX), neosaxitoxin (NEO), decarbamoyl neosaxitoxin (dcNEO), gonyautoxin 1 and 4 (GTX1,4; sum of isomers), gonyautoxin 2 and 3 (GTX2,3; sum of isomers), gonyautoxin 5 (GTX5), gonyautoxin 6 (GTX6), decarbamoyl gonyautoxin 2 and 3 (dcGTX2,3; sum of isomers), N-sulfocarbamoyl gonyautoxin 2 and 3 (C1,2; sum of isomers) and N-sulfocarbamoyl gonyautoxin 1 and 4 (C3,4; sum of isomers) in (raw) mussels, oysters, scallops and clams. Laboratory experience has shown that this document can also be applied to other marine invertebrates [2], [3] and processed products of those species, however, no complete interlaboratory validation study according to ISO 5725-2 [21] has been carried out so far. The method described was validated in an interlaboratory study [4], [5] and was also verified in a European Union Reference Laboratory for Marine Biotoxins (EURLMB)-performance test aiming the total toxicity of the samples [6]. Toxins which were not available in the first interlaboratory study [4], [5] as dcGTX2,3 and dcNEO were validated in two additional interlaboratory studies [7], [8]. The lowest validated levels [4], [5], [8], are given as mass fraction of toxin (free base) in µg/kg shellfish tissue and also as µmol/kg shellfish tissue and are listed in Table 1.
[Table 1 - Lowest validated levels]
A quantitative determination of GTX6 was not included in the first interlaboratory study but several laboratories detected this toxin directly after solid phase extraction with ion-exchange (SPE-COOH) clean-up and reported a mass fraction (free base) of 30 µg/kg or higher in certain samples. For that reason, the present method is applicable to quantify GTX6 directly, depending on the availability of the standard substance. Whenever GTX6 standard is not commercially available, it is possible to determine GTX6 after hydrolysis of Fraction 2 of the SPE-COOH clean-up, described in 7.4, as NEO. The indirect quantification of GTX6 was validated in two additional interlaboratory studies [7], [8]. A study to compare direct and indirect GTX6 quantification was conducted at the EURLMB [16].
A quantitative determination of C3,4 was included in the first interlaboratory study. The present method is applicable to quantify C3,4 directly, depending on the availability of the standard substance. If no standard substances are available, C3,4 can only be quantified as GTX1,4 if the same hydrolysis protocol used for GTX6 (7.4) is applied to Fraction 1 of the SPE-COOH clean-up [10]. A study to compare direct and indirect C3,4 quantification was conducted at the EURLMB [16].

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This document specifies a procedure to determine the release of metals from metallic materials used in products intended to come into contact with drinking water.
The test can be used for three purposes:
a)   To assess a material as a reference material for a new category of materials by metal release testing using the results of several investigations in different waters covering a broad range of water compositions;
b)   To assess a material for an existing category for approval by way of metal release testing using the water defined in part 2, which exhibited the highest metal release when the reference material of the category was tested;
c)   To obtain data on the interaction of local water with a material.

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This document specifies a procedure for obtaining a migration water to determine odour, flavour, colour and turbidity for products made from organic materials intended to come in contact with water for human consumption (drinking water) and used in piping and storage systems. Such products include pipes, tanks, reservoirs, fittings, ancillaries and their coatings both for site applied and factory-made products.
This document is applicable to products to be used under various conditions for the transport, storage and distribution of water intended for human consumption and raw water used for the manufacture of water intended for human consumption.
This document specifies a test method comprising a set of procedures. The use might be dependent on the relevant national regulations and/or the system or product standards.

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This document specifies the requirements of winter savory (Satureja montana Linnaeus) in the form of sprigs, and whole or broken leaves. It does not apply to summer savory (Satureja horfensis Linnaeus), which forms the subject of ISO 7928-2. Recommendations relating to storage and transport conditions are given in Annex A.

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This document specifies the requirements of summer savory (Satureja hortensis Linnaeus) in the form of sprigs, and whole or broken leaves. It does not apply to winter savory (Satureja montana Linnaeus), which forms the subject of ISO 7928-1. Recommendations relating to storage and transport conditions are given in Annex A.

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This document specifies the requirements for dried thyme (Thymus vulgaris L.) in the form of rubbed leaves. Recommendations relating to storage and transport conditions are given in Annex A.

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This document specifies a method of preparing a ground sample of spice or condiment for analysis, from a laboratory sample obtained by the method specified in ISO 948. This document is applicable to the majority of spices and condiments. However, in view of the large number and diversity of spices and condiments, it can be necessary in certain special cases, for example, considerable hardness, or high moisture, volatile oil or fat content, to use a modified procedure or to choose another more suitable method. Any such modified procedure or alternative method will be indicated in the International Standard appropriate to the spice or condiment concerned.

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This document specifies a method for the determination of the ash content in milk, milk powder, whey powder, whey protein concentrate, infant formula, milk permeate powder and milk protein concentrate. NOTE For the determination of ash (“fixed ash”) of caseins and caseinates, see ISO 5544 | IDF 89.

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This document specifies a method for the selective enumeration of bifidobacteria in milk products by using a colony-count technique at 37 °C under anaerobic conditions.
The method is applicable to milk products, such as fermented (e.g. yoghurts) and non-fermented milks (e.g. pasteurized milks, skim milks, whey protein concentrates), milk powders and formulae (e.g. infant formulae, follow-up formulae for older infants, products for young children) where these microorganisms are present and viable, in combination with other lactic acid bacteria or alone. The method is also applicable to starter and probiotic cultures. For proposed quality criteria of dairy products, see, for example, CXS 243-2003.
Bifidobacteria used in milk products usually belong to the following species (e.g. References [7] and [10]):
—     Bifidobacterium adolescentis;
—     B. animalis subsp. animalis;
—     B. animalis subsp. lactis;
—     B. bifidum;
—     B. breve;
—     B. longum subsp. infantis;
—     B. longum subsp. longum.

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This document specifies a method for the determination of fatty acid methyl esters (FAMEs) derived by transesterification or esterification from fats, oils, and fatty acids by capillary gas chromatography (GLC). FAMEs from C4 to C24 can be separated using this document including saturated FAMEs, cis- and trans-monounsaturated FAMEs, and cis- and trans-polyunsaturated FAMEs.
This document is applicable to crude, refined, partially hydrogenated or fully hydrogenated fats, oils and fatty acids derived from animal and vegetable sources, and fats extracted from foodstuff.
This document does not apply to milk and milk products (or fat coming from milk and milk products) or products supplemented with conjugated linoleic acid (CLA).
This document does not apply to di-, tri-, polymerized, hydroxylated and oxidized fatty acids, and fats and oils.
A method for the determination of the composition of FAMEs expressed by area % in liquid vegetable oils is proposed in Annex E.

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This document specifies a method [1] for the quantitative determination of saxitoxin (STX), decarbamoyl saxitoxin (dcSTX), neosaxitoxin (NEO), decarbamoyl neosaxitoxin (dcNEO), gonyautoxin 1 and 4 (GTX1,4; sum of isomers), gonyautoxin 2 and 3 (GTX2,3; sum of isomers), gonyautoxin 5 (GTX5), gonyautoxin 6 (GTX6), decarbamoyl gonyautoxin 2 and 3 (dcGTX2,3; sum of isomers), N-sulfocarbamoyl gonyautoxin 2 and 3 (C1,2; sum of isomers) and N-sulfocarbamoyl gonyautoxin 1 and 4 (C3,4; sum of isomers) in (raw) mussels, oysters, scallops and clams. Laboratory experience has shown that this document can also be applied to other marine invertebrates [2], [3] and processed products of those species, however, no complete interlaboratory validation study according to ISO 5725-2 [21] has been carried out so far. The method described was validated in an interlaboratory study [4], [5] and was also verified in a European Union Reference Laboratory for Marine Biotoxins (EURLMB)-performance test aiming the total toxicity of the samples [6]. Toxins which were not available in the first interlaboratory study [4], [5] as dcGTX2,3 and dcNEO were validated in two additional interlaboratory studies [7], [8]. The lowest validated levels [4], [5], [8], are given as mass fraction of toxin (free base) in µg/kg shellfish tissue and also as µmol/kg shellfish tissue and are listed in Table 1.
[Table 1 - Lowest validated levels]
A quantitative determination of GTX6 was not included in the first interlaboratory study but several laboratories detected this toxin directly after solid phase extraction with ion-exchange (SPE-COOH) clean-up and reported a mass fraction (free base) of 30 µg/kg or higher in certain samples. For that reason, the present method is applicable to quantify GTX6 directly, depending on the availability of the standard substance. Whenever GTX6 standard is not commercially available, it is possible to determine GTX6 after hydrolysis of Fraction 2 of the SPE-COOH clean-up, described in 7.4, as NEO. The indirect quantification of GTX6 was validated in two additional interlaboratory studies [7], [8]. A study to compare direct and indirect GTX6 quantification was conducted at the EURLMB [16].
A quantitative determination of C3,4 was included in the first interlaboratory study. The present method is applicable to quantify C3,4 directly, depending on the availability of the standard substance. If no standard substances are available, C3,4 can only be quantified as GTX1,4 if the same hydrolysis protocol used for GTX6 (7.4) is applied to Fraction 1 of the SPE-COOH clean-up [10]. A study to compare direct and indirect C3,4 quantification was conducted at the EURLMB [16].

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This document specifies a test method for estimating the transfer of mineral oil saturated hydrocarbons (MOSH) and mineral oil aromatic hydrocarbons (MOAH) from food contact materials containing recycled pulp.
This test method is applicable for examining the extent of migration from paper and board equipped with a barrier or other technical solutions to reduce the amount of migration.
This test method is also applicable to paper and board made from virgin fibres.

  • Technical specification
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  • Technical specification
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This document specifies a method for the analysis of pesticide residues in foods of animal origin with a low fat content, such as meat/muscle, egg or milk by LC-MS/MS. Because of the low material requirements for miniaturized processing and the few work steps, the process is particularly time and cost-saving with high reliability and effectiveness. The method has been collaboratively studied on a number of commodity/pesticide combinations. Precision data are summarized in Table B.1. Guidelines for calibration are outlined in CEN/TS 17061:2019.

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