This document specifies a method [1] for the quantitative determination of saxitoxin (STX), decarbamoyl saxitoxin (dcSTX), neosaxitoxin (NEO), decarbamoyl neosaxitoxin (dcNEO), gonyautoxin 1 and 4 (GTX1,4; sum of isomers), gonyautoxin 2 and 3 (GTX2,3; sum of isomers), gonyautoxin 5 (GTX5), gonyautoxin 6 (GTX6), decarbamoyl gonyautoxin 2 and 3 (dcGTX2,3; sum of isomers), N-sulfocarbamoyl gonyautoxin 2 and 3 (C1,2; sum of isomers) and N-sulfocarbamoyl gonyautoxin 1 and 4 (C3,4; sum of isomers) in (raw) mussels, oysters, scallops and clams. Laboratory experience has shown that this document can also be applied to other marine invertebrates [2], [3] and processed products of those species, however, no complete interlaboratory validation study according to ISO 5725-2 [21] has been carried out so far. The method described was validated in an interlaboratory study [4], [5] and was also verified in a European Union Reference Laboratory for Marine Biotoxins (EURLMB)-performance test aiming the total toxicity of the samples [6]. Toxins which were not available in the first interlaboratory study [4], [5] as dcGTX2,3 and dcNEO were validated in two additional interlaboratory studies [7], [8]. The lowest validated levels [4], [5], [8], are given as mass fraction of toxin (free base) in µg/kg shellfish tissue and also as µmol/kg shellfish tissue and are listed in Table 1.
[Table 1 - Lowest validated levels]
A quantitative determination of GTX6 was not included in the first interlaboratory study but several laboratories detected this toxin directly after solid phase extraction with ion-exchange (SPE-COOH) clean-up and reported a mass fraction (free base) of 30 µg/kg or higher in certain samples. For that reason, the present method is applicable to quantify GTX6 directly, depending on the availability of the standard substance. Whenever GTX6 standard is not commercially available, it is possible to determine GTX6 after hydrolysis of Fraction 2 of the SPE-COOH clean-up, described in 7.4, as NEO. The indirect quantification of GTX6 was validated in two additional interlaboratory studies [7], [8]. A study to compare direct and indirect GTX6 quantification was conducted at the EURLMB [16].
A quantitative determination of C3,4 was included in the first interlaboratory study. The present method is applicable to quantify C3,4 directly, depending on the availability of the standard substance. If no standard substances are available, C3,4 can only be quantified as GTX1,4 if the same hydrolysis protocol used for GTX6 (7.4) is applied to Fraction 1 of the SPE-COOH clean-up [10]. A study to compare direct and indirect C3,4 quantification was conducted at the EURLMB [16].

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This document specifies a method [1] for the quantitative determination of saxitoxin (STX), decarbamoyl saxitoxin (dcSTX), neosaxitoxin (NEO), decarbamoyl neosaxitoxin (dcNEO), gonyautoxin 1 and 4 (GTX1,4; sum of isomers), gonyautoxin 2 and 3 (GTX2,3; sum of isomers), gonyautoxin 5 (GTX5), gonyautoxin 6 (GTX6), decarbamoyl gonyautoxin 2 and 3 (dcGTX2,3; sum of isomers), N-sulfocarbamoyl gonyautoxin 2 and 3 (C1,2; sum of isomers) and N-sulfocarbamoyl gonyautoxin 1 and 4 (C3,4; sum of isomers) in (raw) mussels, oysters, scallops and clams. Laboratory experience has shown that this document can also be applied to other marine invertebrates [2], [3] and processed products of those species, however, no complete interlaboratory validation study according to ISO 5725-2 [21] has been carried out so far. The method described was validated in an interlaboratory study [4], [5] and was also verified in a European Union Reference Laboratory for Marine Biotoxins (EURLMB)-performance test aiming the total toxicity of the samples [6]. Toxins which were not available in the first interlaboratory study [4], [5] as dcGTX2,3 and dcNEO were validated in two additional interlaboratory studies [7], [8]. The lowest validated levels [4], [5], [8], are given as mass fraction of toxin (free base) in µg/kg shellfish tissue and also as µmol/kg shellfish tissue and are listed in Table 1.
[Table 1 - Lowest validated levels]
A quantitative determination of GTX6 was not included in the first interlaboratory study but several laboratories detected this toxin directly after solid phase extraction with ion-exchange (SPE-COOH) clean-up and reported a mass fraction (free base) of 30 µg/kg or higher in certain samples. For that reason, the present method is applicable to quantify GTX6 directly, depending on the availability of the standard substance. Whenever GTX6 standard is not commercially available, it is possible to determine GTX6 after hydrolysis of Fraction 2 of the SPE-COOH clean-up, described in 7.4, as NEO. The indirect quantification of GTX6 was validated in two additional interlaboratory studies [7], [8]. A study to compare direct and indirect GTX6 quantification was conducted at the EURLMB [16].
A quantitative determination of C3,4 was included in the first interlaboratory study. The present method is applicable to quantify C3,4 directly, depending on the availability of the standard substance. If no standard substances are available, C3,4 can only be quantified as GTX1,4 if the same hydrolysis protocol used for GTX6 (7.4) is applied to Fraction 1 of the SPE-COOH clean-up [10]. A study to compare direct and indirect C3,4 quantification was conducted at the EURLMB [16].

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This document specifies a method for the analysis of pesticide residues in foods of animal origin with a low fat content, such as meat/muscle, egg or milk by LC-MS/MS. Because of the low material requirements for miniaturized processing and the few work steps, the process is particularly time and cost-saving with high reliability and effectiveness. The method has been collaboratively studied on a number of commodity/pesticide combinations. Precision data are summarized in Table B.1. Guidelines for calibration are outlined in CEN/TS 17061:2019.

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This document specifies a method for the analysis of pesticide residues in foods of animal origin with a low fat content, such as meat/muscle, egg or milk by LC-MS/MS. Because of the low material requirements for miniaturized processing and the few work steps, the process is particularly time and cost-saving with high reliability and effectiveness. The method has been collaboratively studied on a number of commodity/pesticide combinations. Precision data are summarized in Table B.1. Guidelines for calibration are outlined in CEN/TS 17061:2019.

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This document specifies a semi-micro nitrogen determination method for total volatile basic nitrogen in meat, including livestock and poultry, and fish products.

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This document specifies the determination of nitrite and nitrate content by continuous flow analysis (CFA) method in meat, poultry and their products.

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This document specifies a real-time PCR procedure for the quantitation of the amount of roe deer DNA relative to total mammalian DNA in meat and meat products.
Results of this roe deer assay are expressed in terms of roe deer (Capreolus capreolus) haploid genome copy numbers relative to total mammalian haploid genome copy numbers. The content of roe deer can also be expressed as mass fraction in % using gravimetrically prepared calibration material from meat mixtures or model samples.
The method has been previously validated in a collaborative study and applied to DNA extracted from samples that consist of raw roe deer meat in a raw pig meat background as well as raw and boiled sausages.
The limit of detection of the roe deer PCR has been determined experimentally to be at least 5 target gene copies or at least 0,1 % roe deer.
The compliance assessment process is not part of this document.

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This document specifies the production and hygiene requirements for dry-cured ham and establishes a system of analytical methods to ensure the quality of dry-cured ham. This document also specifies the requirements for transport, storage, packaging and labelling. This document is applicable to both dry-cured ham and non-ready-to-eat dry-cured ham. This document is also applicable to dry-cured ham production.

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This document specifies a real-time PCR procedure for the quantitation of the amount of roe deer DNA relative to total mammalian DNA in meat and meat products.
Results of this roe deer assay are expressed in terms of roe deer (Capreolus capreolus) haploid genome copy numbers relative to total mammalian haploid genome copy numbers. The content of roe deer can also be expressed as mass fraction in % using gravimetrically prepared calibration material from meat mixtures or model samples.
The method has been previously validated in a collaborative study and applied to DNA extracted from samples that consist of raw roe deer meat in a raw pig meat background as well as raw and boiled sausages.
The limit of detection of the roe deer PCR has been determined experimentally to be at least 5 target gene copies or at least 0,1 % roe deer.
The compliance assessment process is not part of this document.

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This document specifies requirements for calculating the carbon footprint specific to farmed macroalgae product category rules (CFP–PCR). This methodology builds on the requirements of International Standards for life cycle assessment (LCA) and products’ carbon footprints. This document is applicable to the calculation and communication of farmed macroalgae products’ carbon footprints from seedling production to the consumption of macroalgae products. It is applicable to the carbon footprints of products from aquaculture value chains. This document used alone does not apply to specifying a product’s overall environmental or sustainability characteristics.

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This document specifies requirements for rapid determination of moisture of fresh meat based on low-field nuclear magnetic resonance (LF-NMR) technology. This document is applicable to the rapid detection of moisture in fresh meat.

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This document specifies the requirements for quick-frozen coated aquatic products and test methods for quality control. It also specifies the requirements of packaging, labelling, storage and transportation. This document is applicable to raw or pre-cooked products made from a single species of finfish, crustaceans, cephalopods or other aquatic animals, mainly through pre-treatment, wet and/or dry coating with batter and/or breading and quick freezing.

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This document specifies a real-time PCR procedure for the quantitation of the amount of equine DNA relative to total mammalian DNA in a raw meat sample.
Results of this equine assay are expressed in terms of equine (Equus genus) haploid genome copy numbers relative to total mammalian haploid genome copy numbers. This assay is specific for representatives of the genus Equus and therefore detects horse, mule, donkey and zebra DNA.
The method has been previously validated in a collaborative study and applied to DNA extracted from samples that consist of raw horse meat in a raw beef (meat) background.
The limit of detection has been determined experimentally to be at least 17 horse haploid genome equivalents (HGE) for both the equine PCR and the mammalian PCR based on the lowest dilution on the respective calibration curves through single laboratory validation. The lowest relative horse content of the target sequence included in the collaborative study was a mass fraction of 0,1 % based on gravimetrically prepared raw horse muscle tissue in a raw beef muscle tissue background.
The compliance assessment process is not part of this document.

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This document specifies a real-time PCR procedure for the quantitation of the amount of equine DNA relative to total mammalian DNA in a raw meat sample.
Results of this equine assay are expressed in terms of equine (Equus genus) haploid genome copy numbers relative to total mammalian haploid genome copy numbers. This assay is specific for representatives of the genus Equus and therefore detects horse, mule, donkey and zebra DNA.
The method has been previously validated in a collaborative study and applied to DNA extracted from samples that consist of raw horse meat in a raw beef (meat) background.
The limit of detection has been determined experimentally to be at least 17 horse haploid genome equivalents (HGE) for both the equine PCR and the mammalian PCR based on the lowest dilution on the respective calibration curves through single laboratory validation. The lowest relative horse content of the target sequence included in the collaborative study was a mass fraction of 0,1 % based on gravimetrically prepared raw horse muscle tissue in a raw beef muscle tissue background.
The compliance assessment process is not part of this document.

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This document specifies a method for the identification of single fish and fish fillets to the level of genus or species. It allows the identification of a large number of commercially important fish species using DNA barcoding.
This method was validated on raw fish. Laboratory experience indicates additional applicability to processed fish products (e.g. cold smoked, hot smoked, salted, frozen, cooked, fried and deep-fried samples).
The described method is usually unsuitable for the analysis of highly processed foods (e.g. tins of fish with highly degraded DNA where the fragment lengths are not sufficient for amplification of the targets). Furthermore, it does not apply to complex fish products containing mixtures of two or more fish species.
The identification of fish species is carried out by PCR amplification of either a segment of the mitochondrial cytochrome b gene (cytb) or the cytochrome c oxidase I gene (cox1, syn COI), or both, followed by sequencing of the PCR products and subsequent sequence comparison with entries in databases.

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This document specifies a method for the identification of single fish and fish fillets to the level of genus or species. It allows the identification of a large number of commercially important fish species using DNA barcoding.
This method was validated on raw fish. Laboratory experience indicates additional applicability to processed fish products (e.g. cold smoked, hot smoked, salted, frozen, cooked, fried and deep-fried samples).
The described method is usually unsuitable for the analysis of highly processed foods (e.g. tins of fish with highly degraded DNA where the fragment lengths are not sufficient for amplification of the targets). Furthermore, it does not apply to complex fish products containing mixtures of two or more fish species.
The identification of fish species is carried out by PCR amplification of either a segment of the mitochondrial cytochrome b gene (cytb) or the cytochrome c oxidase I gene (cox1, syn COI), or both, followed by sequencing of the PCR products and subsequent sequence comparison with entries in databases.

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This document specifies a method for the identification of single fish and fish fillets to the level of genus or species. It allows the identification of a large number of commercially important fish species using DNA barcoding. This method was validated on raw fish. Laboratory experience indicates additional applicability to processed fish products (e.g. cold smoked, hot smoked, salted, frozen, cooked, fried and deep-fried samples). The described method is usually unsuitable for the analysis of highly processed foods (e.g. tins of fish with highly degraded DNA where the fragment lengths are not sufficient for amplification of the targets). Furthermore, it does not apply to complex fish products containing mixtures of two or more fish species. The identification of fish species is carried out by PCR amplification of either a segment of the mitochondrial cytochrome b gene (cytb) or the cytochrome c oxidase I gene (cox1, syn COI), or both, followed by sequencing of the PCR products and subsequent sequence comparison with entries in databases.

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This document specifies a method for the taxonomic identification of a single bivalve or piece of bivalve to the genus or species level using DNA barcoding. It allows the identification of a large number of commercially important bivalve species.
This method was validated on raw mussels. Laboratory experience indicates additional applicability to processed bivalve products, e.g. cold smoked, hot smoked, salted, frozen, cooked, fried, and deep-fried samples.
The described method is usually unsuitable for the analysis of highly processed foods, e.g. tins of mussels, with highly degraded DNA where the fragment lengths are not sufficient for amplification of the target. Furthermore, it is not applicable for complex seafood products containing mixtures of two or more bivalve species.
The identification of bivalve species is carried out by PCR amplification of a segment of the mitochondrial 16S rRNA gene, followed by sequencing of the PCR products and subsequent sequence comparison with entries in databases.

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This document specifies a method for the identification of meat derived from mammals and birds to the level of genus or species and allows the identification of a large number of commercially important as well as exotic meat species using DNA barcoding.
This method was validated on DNA isolated from single pieces of raw meat. This method can also be used for the identification of single meat animal species in some processed products.
The described method is unsuitable for the analysis of highly processed foods with highly degraded DNA where the fragment lengths are not sufficient for amplification of the targets. Furthermore, it is not applicable for complex meat products containing mixtures of two or more meat species.
The identification of meat species is carried out by PCR amplification of either a segment of the mitochondrial cytochrome b gene (cytb) or the cytochrome c oxidase I gene (cox1, syn COI) or both, followed by sequencing of the PCR products and subsequent sequence comparison with entries in databases.

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This document specifies a method for the identification of meat derived from mammals and birds to the level of genus or species and allows the identification of a large number of commercially important as well as exotic meat species using DNA barcoding.
This method was validated on DNA isolated from single pieces of raw meat. This method can also be used for the identification of single meat animal species in some processed products.
The described method is unsuitable for the analysis of highly processed foods with highly degraded DNA where the fragment lengths are not sufficient for amplification of the targets. Furthermore, it is not applicable for complex meat products containing mixtures of two or more meat species.
The identification of meat species is carried out by PCR amplification of either a segment of the mitochondrial cytochrome b gene (cytb) or the cytochrome c oxidase I gene (cox1, syn COI) or both, followed by sequencing of the PCR products and subsequent sequence comparison with entries in databases.

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This document specifies a method for the taxonomic identification of a single bivalve or piece of bivalve to the genus or species level using DNA barcoding. It allows the identification of a large number of commercially important bivalve species.
This method was validated on raw mussels. Laboratory experience indicates additional applicability to processed bivalve products, e.g. cold smoked, hot smoked, salted, frozen, cooked, fried, and deep-fried samples.
The described method is usually unsuitable for the analysis of highly processed foods, e.g. tins of mussels, with highly degraded DNA where the fragment lengths are not sufficient for amplification of the target. Furthermore, it is not applicable for complex seafood products containing mixtures of two or more bivalve species.
The identification of bivalve species is carried out by PCR amplification of a segment of the mitochondrial 16S rRNA gene, followed by sequencing of the PCR products and subsequent sequence comparison with entries in databases.

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This document specifies two reference methods for the determination of the moisture content of meat and meat products: a direct drying method and a distillation method.
The direct drying method is applicable to meat and meat products with low volatile substances in addition to moisture.
The distillation method is applicable to meat and meat products with high volatile substances in addition to moisture.

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This document specifies a reference method for the determination of the nitrogen content of meat and meat products by the Kjeldahl principle.

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ABSTRACT
This practice covers determination of the quantitative and qualitative species composition of fish in a specified area. The successful use of this technique is dependent on: (1) preventing fish from escaping the sample area and (2) retrieving all affected fish, which may take up to three days. This practice is useful in both short- and long-term studies for management and impact assessment purposes. The sample area is blocked off with a small mesh net(s) and the volume of water to be treated is calculated. The required quantity of rotenone is diluted and distributed throughout the water column in the sample area. All fish should be affected and they should be collected for processing.
SCOPE
1.1 This practice covers determination of the quantitative and qualitative species composition of fish in a specified area. The successful use of this technique is dependent on: (1) preventing fish from escaping the sample area and (2) retrieving all affected fish, which may take up to three days.  
1.2 Advantages:  
1.2.1 Easily detoxified.  
1.2.2 All native freshwater fish are susceptible, but it has low toxicity to mammals and birds.  
1.2.3 At low concentrations fish toxicity depends on species, age, and size.  
1.2.4 The suffocating action is reversible.  
1.3 Limitations:  
1.3.1 It is less effective in cold (below 20 °C) and highly alkaline water.  
1.3.2 Smaller fish and those without air bladders usually do not float.  
1.3.3 Completely random selection of sample areas is not possible.  
1.3.4 Overkill beyond sample area can sometimes occur.  
1.3.5 Food web organisms may be eliminated.  
1.4 Applications—This practice is useful in both short- and long-term studies for management and impact assessment purposes. It is adaptable to both lotic and lentic situations in littoral and limnetic areas.  
1.5 The values stated in inch-pound units are to be regarded as standard. The values given in parentheses are mathematical conversions to SI units that are provided for information only and are not considered standard.  
1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. For specific hazards, see Section 7.  
1.7 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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This document specifies a method for the detection of linear condensed phosphates in meat and meat products by thin layer chromatographic separation. This document only applies to the detection of added condensed phosphates that are still present in the sample at the time of investigation, because condensed phosphates are gradually hydrolyzed by enzymes present in meat or meat products and during heat treatment of meat or meat products.

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This document specifies a method for the determination of nitrite and nitrate in meat and meat products using ion chromatography.

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This document specifies two reference methods for the determination of the moisture content of meat and meat products: a direct drying method and a distillation method. The direct drying method is applicable to meat and meat products with low volatile substances in addition to moisture. The distillation method is applicable to meat and meat products with high volatile substances in addition to moisture.

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This document specifies a reference method for the determination of the nitrogen content of meat and meat products by the Kjeldahl principle.

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This document specifies a liquid chromatography tandem mass spectrometry method (LC-MS/MS) for the determination of fipronil and metabolites (including fipronil-desulfinyl, fipronil-sulfide and fipronil-sulfone) residues in eggs and egg products.

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This document specifies a procedure for the qualitative detection of species specific DNA from white mustard (Sinapis alba) and soya (Glycine max) in cooked sausages using singleplex real-time PCR based on the genes MADS-D (mustard) and lectin (soya).

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This document specifies a procedure for the qualitative detection of species specific DNA from white mustard (Sinapis alba) and soya (Glycine max) in cooked sausages using singleplex real-time PCR based on the genes MADS-D (mustard) and lectin (soya).

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SIGNIFICANCE AND USE
5.1 Protection of a species requires prevention of unacceptable effects on the number, weight, health, and uses of the individuals of that species. Toxicity tests can be used provide information about the toxicity of a test material to a specific life stage of a particular species of mussel. The primary adverse effects studied are reduced survival or growth.  
5.2 Results of toxicity tests might be used to predict effects likely to occur on mussels in field situations as a result of an exposure under comparable conditions.  
5.3 Results of toxicity tests might be used to compare the sensitivities of different mussel species and the toxicity of different test materials, and to study the effects of various environmental factors on results of such tests.  
5.4 Results of toxicity tests conducted with mussels might be an important consideration when assessing the risks of test materials to aquatic organisms or when deriving environmental guideline values for toxicants.  
5.5 An acute toxicity test is conducted to obtain information concerning the immediate effects on mussels of a short exposure to a test material under specific experimental conditions. An acute toxicity test does not provide information about whether delayed effects will occur, although a post-exposure observation period, with appropriate feeding, if necessary, might provide such information (Guide E729).  
5.6 Results of chronic (at least 28 d) toxicity tests with mussels might be used to predict chronic or partial chronic effects on species in field situations as a result of exposure under comparable conditions.  
5.7 Short-term chronic toxicity tests are conducted for 7 d, a complementary test duration in the USEPA shot-term methods for estimating the chronic toxicity of effluents and receiving waters to fathead minnow (Pimephales promelas; USEPA 2002)  (31) and provides a more direct estimate of the safe concentrations of effluents and receiving waters than acute toxicity tests, at a slightly ...
SCOPE
1.1 This standard guide describes methods for conducting laboratory toxicity tests with early life stages of freshwater mussels including glochidia and juvenile mussels in water-only and effluent exposures (Annex A1). Future revisions to this standard may describe methods for conducting toxicity tests with endpoints of reproduction, behaviors, and biomarkers.  
1.2 Freshwater mussels (order Unionida) are one of the most imperiled groups of animals in the world, and environmental contamination has been linked as a contributing factor to the decline of mussel populations (Lydeard et al. 2004  (1); Strayer et al. 2004  (2); Haag 2012  (3); Lopes-Lima et al. 2017  (4)).2 Three critical life stages (glochidia, juvenile mussels, and adults) have been used in toxicity assessments and the toxicity studies are separated according to the medium of exposure (water, sediment, and host fish (Ingersoll et al. 2007  (5)). Recent studies on early life stages of mussels have demonstrated that the mussels are among the most sensitive freshwater species to a variety of contaminants, including ammonia, some metals (for example, aluminum, copper, nickel, and zinc), and major ions (for example, chloride, nitrate, potassium, and sulfate) (Bringolf et al. 2007  (6); Newton et al. 2007  (7); Wang et al. 2007ab, 2010, 2011ab, 2016, 2017ab, 2018abc, 2020ab (8-20); Cope et al. 2008  (21); Gillis et al. 2008, 2010, 2011, 2021 (22-25); Miao et al. 2010  (26); Salerno et al. 2020  (27)). These studies indicate that environmental guideline values for individual chemicals established for the protection of aquatic organisms may not be adequately protective of sensitive stages of freshwater mussels. For example, when freshwater mussel toxicity data were included in an update to the United States Environmental Protection Agency (USEPA) ambient water quality criteria (WQC) for ammonia, the acute criterion decreased by about a 1.4 fold and the chronic crite...

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SIGNIFICANCE AND USE
4.1 The principal purpose of irradiation is to help ensure the safety of these foods for human consumption. Irradiation significantly reduces the numbers of pathogenic bacteria such as Campylobacter, Shiga toxin-Producing E coli, Listeria monocytogenes, Salmonella, Staphylococcus aureus, and Yersinia enterocolitica.
Note 3: Ionizing radiation doses below 10 kGy will reduce but may not eliminate spores of pathogenic bacteria including those of Clostridium botulinum, Clostridium perfringens, and Bacillus cereus.  
4.2 The process also inactivates parasites such as Trichinella spiralis and Toxoplasma gondii.  
4.3 The process may extend the shelf life of fresh meat and poultry by reducing the numbers of viable, spoilage bacteria, such as Pseudomonas species and lactic acid bacilli.  
4.4 Radiation processing of fresh, frozen, or processed meat and poultry is a critical control point (CCP) of a Hazard Analysis of Critical Control Points (HACCP) program. It serves as an important measure to control any residual risk from pathogenic microorganisms before the product reaches the consumer (4).  
4.5 The “Recommended International Code of Practice for Radiation Processing of Food” (CAC/RCP 19-1979) of the Codex Alimentarius identifies the essential practices to be implemented to achieve effective radiation processing of food, in general, in a manner that maintains quality and yields food products that are safe and suitable for consumption.
SCOPE
1.1 This guide outlines procedures for the irradiation of fresh, frozen, or processed meat and poultry.
Note 1: The Codex Alimentarius Commission defines meat as “the edible part of any mammal” and poultry as “any domesticated bird, including chicken, turkeys, ducks, geese, guinea-fowls, or pigeons” (CAC/MISC 5).
Note 2: Current U.S. regulations limit the definition of meat and poultry as listed in 9 CFR Section 301.2 and 381.1, respectively. (2, 3).  
1.2 This guide covers the use of ionizing radiation to eliminate or reduce the numbers of vegetative, pathogenic microorganisms and parasites, and to extend the refrigerated shelf-life of those products by reducing the numbers of spoilage microorganisms in fresh, frozen, or processed meat and poultry. The absorbed dose for this application is typically less than 10 kGy.  
1.2.1 This guide covers gamma, electron beam, and X-radiation treatment.  
1.3 This guide addresses irradiation of pre-packaged product for retail sale or for use as an ingredient in other products. It also addresses the in-line irradiation of unpackaged product. Other specific ISO and ASTM standards exist for the irradiation of food. In those areas covered by ISO 14470, that standard takes precedence.  
1.4 This document is one of a set of standards that provides recommendations for properly implementing and utilizing radiation processing. It is intended to be read in conjunction with ISO/ASTM 52628.  
1.5 The values stated in inch-pound units are to be regarded as standard. The values given in parentheses are mathematical conversions to SI units that are provided for information only and are not considered standard.  
1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.7 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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This document defines terms for meat and meat products. It is applicable to the processing, trade and storage of meat and meat products.

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This document specifies the requirements for frozen surimi and the test methods for its quality control. It also specifies the requirements of packaging, marking, storage and transportation. This document is applicable to tropical and cold-water surimi.

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Le présent document spécifie les exigences de production et sanitaires applicables aux produits fermentés à base de viande et établit une série de méthodes d’essai destinées à maîtriser la qualité des produits fermentés à base de viande. Il spécifie également les exigences de transport, de stockage, d’emballage et d’étiquetage. Le présent document est applicable aux produits fermentés à base de viande (du type prêt à consommer), notamment la saucisse fermentée, le jambon sec fermenté et d’autres produits fermentés à base de viande. Il est également applicable à la production de viande fermentée et aux relations commerciales.

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This document specifies the liquid chromatographic (LC) method for the determination of chloramphenicol content of muscle tissue of meat, including livestock and poultry. This document specifies the liquid chromatography tandem mass spectrometry method (LC-MS/MS) for the determination of chloramphenicol content of muscle tissue, casing, liver of meat and meat products, including livestock and poultry. This document specifies LC-MS/MS as the reference method. The LC method is suitable for the determination of chloramphenicol content greater than 6,5 mg/kg. LC-MS/MS is suitable for the determination of chloramphenicol content greater than 0,1 μg/kg. Test samples which have deteriorated cannot be analysed with this method.

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This document specifies a detection method using thin-layer chromatography and a determination method using high performance liquid chromatography (HPLC) for synthetic colouring agents in meat and meat products. This document specifies the HPLC method as the reference method. This document is applicable to meat and meat products, including livestock and poultry products. The method using thin-layer chromatography can detect the following colouring agents: Tartrazine, Patent Blue V, Quinoline Yellow, Indigotine, Sunset Yellow FCF, Brilliant Black PN, Amaranth, Black 7984, Ponceau 4R, Fast Green FCF, Erythrosine, Blue VRS. Synonyms and identity numbers of these colouring agents are listed in Annex A. The plant colours and plant extracts which have been observed not to interfere with this method are listed in B.1. Natural colours which in some cases have been shown to interfere with this method are listed in B.2. The method using HPLC can detect the following colouring agents: Tartrazine, Allura Red AC, Amaranth, Brilliant Blue FCF, Ponceau 4R, New Red, Sunset Yellow FCF, Carmoisine, Erythrosine, Indigotine. Chromatograms of these standard reference colours are shown in Annex D.

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This document specifies three methods for the determination of the total phosphorous content of all kinds of meat and meat products, including poultry and livestock: the inductively coupled plasma optical emission spectrometry (ICP-OES) method; the spectrometric method; the gravimetric method. For the ICP-OES method, the limit of detection (LOD) is 1,0 mg/kg and the limit of quantification (LOQ) is 3,0 mg/kg if the mass of the test portion is 0,5 g and the constant volume is 50 ml.

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This document specifies the spectrophotometer method and the light absorption microplate reader method for the determination of the free L-(+)-glutamic acid content of meat and meat products. This document is applicable to meat and meat products, including livestock and poultry products.

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SIGNIFICANCE AND USE
4.1 Absorbed doses of or below 1 kGy can inactivate some parasites, such as the broad fish tapeworm (Dibothrocephalus latus) (2).  
4.2 Absorbed doses below 10 kGy can reduce or eliminate vegetative cells of pathogenic sporeforming and non-sporeforming microorganisms, such as Clostridium spp., Vibrio spp., Salmonellae, Listeria monocytogenes, or Staphylococcus aureus, that may be present in fresh or frozen product.  
4.2.1 Absorbed doses below 10 kGy can reduce the numbers of some spores, but are not adequate to reduce the potential health risk from microbial spores or toxins (3).  
4.3 Absorbed doses below 10 kGy can reduce or eliminate the vegetative cells of sporeforming and non-sporeforming microorganisms, such as Bacillus or Pseudomonas species, that cause spoilage of fresh product, thus extending refrigerated shelf life in many cases (4).
SCOPE
1.1 This guide outlines procedures and operations for the irradiation of raw, untreated, fresh (chilled), or frozen finfish and aquatic invertebrates, while ensuring that the irradiated product is safe and wholesome.  
1.1.1 Aquatic invertebrates include mollusks, crustacea, echinoderms, etc.
1.1.1.1 Mollusks include bivalve shellfish, such as clams, mussels, and oysters; snails; and cephalopods, such as squid and octopus.
1.1.1.2 Crustacea include shellfish such as shrimp, lobster, crabs, prawns and crayfish.
1.1.1.3 Echinoderms include sea urchins and sea cucumbers.  
1.2 This guide covers absorbed doses used to reduce the microbial and parasite populations in aquatic invertebrates and finfish. Such doses typically are below 10 kGy (1).2  
1.2.1 This guide covers gamma, electron beam, and X-radiation treatment.  
1.3 The use of reduced-oxygen packaging (vacuum or modified atmosphere, and including products packed in oil) with irradiated, raw product is not covered by this guide. The anaerobic environment created by reduced-oxygen packaging provides the potential for outgrowth of, and toxin production from, Clostridium botulinum spores.  
1.4 This guide does not cover the irradiation of smoked or dried fish to reduce microbial load or to control insect infestation.  
1.5 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.6 This document is one of a set of standards that provides recommendations for properly implementing and utilizing radiation processing. It is intended to be read in conjunction with ISO/ASTM Practice 52628.  
1.7 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.8 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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This document specifies a method for the detection of Anisakidae L3 larvae commonly found in marine and anadromous fishes. The method is applicable to fresh fish and/or frozen fish, as well as lightly processed fish products, such as marinated, salted or smoked. It is also suitable for visceral organs as a confirmatory method for a visual inspection scheme.
The artificial digestion method[4][5][6] is applicable to quantifying parasitic infections by estimating the number of parasites in the fish musculature and, when applied to fresh fish or lightly processed fish products (never frozen before processing), determining the viability of Anisakidae L3, which can be present.
This method does not apply to determining the species or genotype of detected parasites. Final identification is made by morphological and/or molecular methods.

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  • Standard
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This document specifies a method for the detection of Anisakidae L3 larvae commonly found in marine and anadromous fishes. The method is applicable to fresh fish and/or frozen fish, as well as lightly processed fish products, such as marinated, salted or cold smoked.
This method is applicable to quantifying parasitic infections by estimating the number of parasites in the fish musculature.
This method does not apply to determining the species or genotype of detected parasites. Final identification is made by morphological and/or molecular methods.

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SCOPE
1.1 This specification covers the requirements for design and construction of evaluation devices or systems for measuring composition or quality constituents of live animals, livestock and poultry carcasses, and individual cuts of meat, or a combination thereof. Examples include, but are not limited, to half and whole carcasses, primals, subprimals, and boxed meat.  
1.2 The values stated in either SI units or inch-pound units are to be regarded separately as standard. The values stated in each system may not be exact equivalents; therefore, each system shall be used independently of the other. Combining values from the two systems may result in non-conformance with the standard.  
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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SIGNIFICANCE AND USE
4.1 Characteristics of livestock, meat, and poultry can be used to determine value. Devices have been and are currently under development to evaluate these characteristics. The use of this test method will assist manufacturers, users, and regulating authorities to refer to uniform test methods to determine if the devices are accurate.
SCOPE
1.1 This test method covers test methods used to determine the accuracy of electronic devices that evaluate composition or quality constituents of livestock, meat, and poultry.  
1.2 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.  
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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SCOPE
1.1 This terminology contains related definitions and descriptions of terms used or likely to be used in livestock, meat, and poultry evaluation standards. The purpose of terminology is to promote clear understanding and interpretation of the standards in which they are used.  
1.2 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.3 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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SIGNIFICANCE AND USE
4.1 There are several live animal or carcass evaluation devices or systems used or under development for use in determination of economic value of live animals, carcasses, or individual cuts of meat. The correct use of these devices or systems may affect the reliability of the data.  
4.2 This practice provides user requirements for installation, operator training, operation, verification, inspection, maintenance, and retention of original data.
SCOPE
1.1 This practice covers the requirements for users of livestock, meat, and poultry evaluation devices or systems used to measure and record composition or quality constituents of live animals, carcasses, and individual cuts of meat, when those devices or systems provide data that is used to determine economic value. Areas covered include: installation, operator training, operation, verification, inspection and maintenance of these evaluation devices or systems, and documentation of procedures for retention of original data.  
1.2 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.3 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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This document specifies requirements for calculating the carbon footprint specific to finfish product category rules (CFP?PCR). This methodology builds on the requirements of International Standards for life cycle assessment (LCA) and products' carbon footprints. This document is applicable to the calculation and communication of finfish products' carbon footprints from fishing and/or cultivation of feed ingredients to the consumption of finfish products. It is applicable to the carbon footprints of products from both fisheries and aquaculture value chains. This document used alone does not apply to specifying a product's overall environmental or sustainability characteristics.

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  • Standard
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SIGNIFICANCE AND USE
5.1 This procedure is used to determine the effects of water-related contaminants on the odor and taste of exposed fish. This procedure may be used as evidence in showing compliance with regulatory procedures.  
5.2 This guide is designed for use by fish processors or research laboratories for evaluations by a trained and monitored sensory panel under the supervision of a sensory professional.
SCOPE
1.1 This guide covers procedures for determination of the effects of water-related contaminants on the odor and taste of live fish or fishery products after alleged exposure where flavor impairment is a suspected issue.  
1.2 This guide addresses safety, harvested quality, sample preparation, assessor selection and training, testing procedures with assessor instructions, as well as test environment and parameters.  
1.3 This guide is applicable to product categories from aquaculture and wild-caught sectors. The range of contaminated products could be from a small-scale water source, such as an estuary, or a limited river system, to a large-scale source, such as a bay, gulf or portion of an ocean. For details on how these methods compare to field- or laboratory-exposed fishery samples, see Ref (1).2  
1.4 Also covered in this guide are fish species, harvest method (wild-caught versus aquaculture/farmed fish), post-harvest handling, processing methods, and storage.  
1.5 This guide provides suggested procedures and is not meant to exclude alternate procedures that may be effective. It also does not address all of the nuances of testing throughout the world. It is the responsibility of the user to be aware of their local guidelines and apply them as needed. Some useful resources are also cited in this guide.  
1.6 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.7 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. Specific hazards statements are given in Section 7.  
1.8 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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