E48 - Bioenergy and Industrial Chemicals from Biomass

SIGNIFICANCE AND USE
4.1 The procedure, in brief, consists of solubilization of biomass using cold caustic extraction, an enzymatic removal of starch and yeast glucan followed by precipitation of hemicellulosic material, and acid hydrolysis of the residual carbohydrate pellet before measurement of glucose and galactose derived from cellulose and hemicellulose.  
4.2 The starch removal procedure is identical to that employed in the NREL assay2 which was itself adapted from the Megazyme-published starch analysis procedure: RTS-NaOH, 2019.5,6  
4.3 The cellulosic pellet hydrolysis and monosaccharide measurements are identical to those described in the NREL laboratory analytical procedure “Determination of Structural Carbohydrates and Lignin in Biomass,” NREL/TP-5100-42618 and “Determination of Cellulosic Glucan Content in Starch Containing Feedstocks,” NREL/TP-2800-76724.7  
4.4 This test method references Practices E1757 and E3181.  
4.5 This test method is intended for use in the measurement of the fermentable portion of cellulosic content in pre- and post-fermentation corn biomass specifically for the apportionment of a percentage of the total ethanol produced in a fermentation as cellulosic.
SCOPE
1.1 This procedure can be used to quantify the cellulose/hemicellulose-derived glucan and galactan (CHDGG) content in corn biomass samples that also contain varying levels of starch-derived and yeast-derived glucan. The method has been shown to provide accurate values for samples with cellulose content up to 40 % w/w.3  
1.2 Units—The values stated in SI units are to be regarded as the standard. No other units of 34 measurement are included in this standard.  
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SCOPE
1.1 This document is composed of terms, definitions of terms, descriptions of terms, and acronyms used in ASTM documents related to the field of bioenergy and industrial chemicals from biomass. Terms that are adequately defined in a general dictionary are not defined in this terminology standard.  
1.2 This standard includes terminology used in areas related to bioenergy and industrial chemicals from biomass, such as, but not limited to: characterization and identification of biomass, aseptic sampling, preservation of biological samples, sustainability, denatured fuel ethanol, cooking fuels and biomass conversion.  
1.2.1 The bylaws for Committee E48 allow the definitions approved in current E48 standards to be added to this terminology standard editorially. The definitions will have an attribution to indicate the standard(s) containing the definition. Subcommittee E48.91 can also develop definitions for the terminology standard. Those definitions will be attributed to the subcommittee.  
1.3 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
5.1 Manufacturers of ethanol are responsible for identifying and controlling impurities according to regulatory standards. Impurities in ethanol that are non-volatile are critical quality attributes for applications in the food, feed and pharmaceutical, personal care applications. Non-volatile residue is an attribute important to users of ethanol for these applications.
SCOPE
1.1 This test method covers the determination of the non-volatile residue content of ethanol and ethanol solutions at the time of test.  
1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.2.1 The accepted SI unit of pressure is the Pascal (Pa); the accepted SI unit for temperature is degrees Celsius.  
1.3 Warning—Mercury has been designated by many regulatory agencies as a hazardous substance that can cause serious medical issues. Mercury, or its vapor, has been demonstrated to be hazardous to health and corrosive to materials. Use caution when handling mercury and mercury-containing products. See the applicable product Safety Data Sheet (SDS) for additional information. The potential exists that selling mercury or mercury-containing products, or both, is prohibited by local or national law. Users must determine legality of sales in their location.  
1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. For specific warning statements, see 6.4, 7.4, and 9.1.  
1.5 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

ABSTRACT
This specification provides requirements and corresponding test methods for denatured ethanol intended to be used as a cooking or appliance fuel, or both. The addition of a denatonium benzoate and a colored dye is required. The minimum and maximum requirements for the amount in which denatonium benzoate must be added are specified. The hydrocarbons approved for use under this specification are gasoline, unleaded gasoline, natural gasoline, heptane, or rubber hydrocarbon solvent.
Also addressed in this specification are workmanship, sampling, sontainers, and sample handling. The test methods cover ethanol, water, and higher alcohols as well as acidity and solvent-washed gum content.
SCOPE
1.1 This specification covers denatured ethanol intended to be used as a cooking or appliance fuel, or both.  
1.1.1 This product is intentionally denatured as an additional deterrent to consumption and to avoid beverage alcohol use.  
1.1.2 This specification allows for various denaturants to be used for the intended purpose of cooking or appliance fuel. Jurisdictions may vary in their regulatory requirement for the allowable or prohibited types of denaturants, chemical composition of the denaturant or concentration of denaturant needed to denature the ethanol.  
1.2 Nothing in this specification shall preclude observance of federal, state, or local regulations.  
1.3 Denatured ethanol has many regulatory limitations that cover the production, trading, transporting, distributing, wholesale and retail sale, and use of denatured ethanol; this specification does not purport to address the regulatory compliance aspects of these activities.  
1.4 The application and intent of this product is for household cooking fuel and not for on-road motor fuel use (see D4806).  
1.5 Units—The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.7 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
5.1 This test method measures acidity quantitatively. Very dilute aqueous solutions of low molecular mass organic acids, such as acetic acid, are highly corrosive to many metals.  
5.2 Acceptable levels of acidity in ethanol can vary with different specifications, but in general, it is below 200 mg/kg (ppm). Knowledge of the acidity can be required to establish whether the product quality meets specification.
SCOPE
1.1 This test method covers the determination of acidity as acetic acid in ethanol and ethanol solutions. This test method may be applied to ethanol samples containing very little water (dehydrated alcohols) to solutions with significant water (low proof ethanol samples). The solutions include denatured alcohols such as the U.S. denatured ethanol formulas defined in 27.CFR.Part 21. This test method is used for determining low levels of acidity, below 200 m⁄kg (ppm mass), with the exclusion of carbon dioxide.  
1.1.1 Procedure A—Developed specifically for measurement of acidity by potentiometric titration. This is the referee method.  
1.1.2 Procedure B—Developed specifically for measurement of acidity by color end point titration.  
1.2 The ethanol may be analyzed directly by this test method with minimal sample preparation.  
1.3 Review the current and appropriate Safety Data Sheets (SDS) for detailed information concerning toxicity, first aid procedures, and safety precautions and proper personal protective equipment.  
1.4 Units—The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. Some specific hazards statements are given in Section 7 on Hazards.  
1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
4.1 Ethanol extractives are any materials found in biomass that are soluble in ethanol. They are not considered to be part of the structural components of biomass and should be removed prior to any chemical analysis of the sample. The prolonged extraction removes nonstructural materials that can include waxes, fats, resins, tannins, gums, sugars, starches, and pigments. Removing hydrophobic materials from the biomass makes it easier to wet the material for the analysis of structural components in the biomass.  
4.2 This test method yields results comparable to Test Method D1107.
SCOPE
1.1 This test method covers the determination of ethanol soluble extractives, expressed as a percentage of the oven-dried biomass, of hard and soft woods, herbaceous materials (such as switchgrass and sericea), agricultural residues (such as corn stover, wheat straw, and bagasse), and wastepaper (such as office waste, boxboard, and newsprint). All results are reported relative to the oven-dried weight of the sample.
Note 1: This test method may not be suitable for hard and soft wood samples that include bark or those with more than 10% extractable materials.  
1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
4.1 The structural carbohydrate content is used in conjunction with other assays to determine the total composition of biomass samples.
SCOPE
1.1 This test method describes the determination of structural carbohydrates present in a biomass sample, expressed as the percent mass of an oven-dried sample basis of each anhydrosugar.  
1.2 Sample materials suitable for this procedure include hard and softwoods, herbaceous materials, such as sericea and switchgrass, agricultural residues, such as corn stover, wheat straw, and bagasse, wastepaper, such as boxboard, office waste, and newsprint, acid or alkaline-pretreated biomass, washed free of any residual acid or alkali, and the solid fraction of fermentation residues.  
1.3 The options for the types of samples to be analyzed in this procedure are:  
1.3.1 Prepared Biomass Samples:  
1.3.1.1 Air Dried Material—Results are reported as the percent by mass, based on the oven-dried mass of the air-dried sample.
1.3.1.2 45 °C Dried Material—Results are reported as the percent by mass, based on the oven-dried mass of the 45 °C dried sample.
1.3.1.3 Freeze Dried Material—Results are reported as the percent by mass, based on the oven-dried mass of the freeze dried sample.  
1.3.2 Extractives-Free Sample—Results are reported as the percent by mass, based on the oven-dried mass of the extracted sample.  
1.4 This standard method is generally not suitable for samples that contain soluble, nonstructural carbohydrates unless they are removed prior to analysis.  
1.5 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  See Section 8 for specific hazards statements.  
1.7 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
4.1 The ash content is an approximate measure of the mineral content and other inorganic matter in biomass.  
4.2 The ash content is used in conjunction with other assays to determine the total composition of biomass samples.
SCOPE
1.1 This test method covers the determination of ash, expressed as the mass percent of residue remaining after dry oxidation (oxidation at 575 ± 25 °C), of hard and soft woods, herbaceous materials (such as switchgrass and sericea), agricultural residues (such as corn stover, wheat straw, and bagasse), wastepaper (such as office waste, boxboard, and newsprint), acid and alkaline pretreated biomass, and the solid fraction of fermentation residues. All results are reported relative to the 105 °C oven-dried mass of the sample. For particulate wood fuels, Test Method E1534 should be used.  
1.2 The values stated in SI units are to be regarded as the standard.  
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
4.1 The acid-insoluble residue content is used in conjunction with other assays to determine the total composition of biomass samples.
SCOPE
1.1 This test method covers determination of the acid-insoluble residue of hard and soft woods, herbaceous materials (such as switchgrass and sericea), agricultural residues (such as corn stover, wheat straw, and bagasse), wastepaper (such as office waste, boxboard, and newsprint), acid and alkaline pretreated biomass, and the solid fraction of fermentation residues. All results are reported relative to the 105 °C oven-dried weight of the sample.  
1.2 The residue collected contains the acid-insoluble lignin and any condensed proteins from the original sample. An independent nitrogen analysis would be required to determine the acid-insoluble lignin content separate from the condensed protein fraction and is outside the scope of this test method.  
1.3 A portion of the lignin in some biomass samples will remain soluble during this procedure. The total lignin in a biomass sample includes both acid-soluble lignin and lignin in the acid insoluble residue.  
1.4 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. Specific hazards statements are given in Section 8 and Note 2 and Note 4.  
1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
4.1 The percentage, by mass, of sugar content is used in conjunction with other assays to determine the total composition of biomass samples.
SCOPE
1.1 This test method covers the determination of carbohydrates present in a biomass sample, expressed as the percent, by mass, of each sugar on a 105 °C dried mass basis.
Note 1: The percent sugar must be corrected for the water of hydrolysis before calculating the actual mass percent of the polysaccharide in the original biomass sample.  
1.2 Sample materials suitable for this procedure include hard and soft woods, herbaceous materials (such as switchgrass and sericea), agricultural residues (such as corn stover, wheat straw, and bagasse), wastepaper (such as office waste, boxboard, and newsprint), acid or alkaline-pretreated biomass (washed free of any residual acid or alkali), and the solid fraction of fermentation residues. All results are reported relative to the 105 °C oven-dried mass of the sample.  
1.3 The options for the types of samples to be analyzed in this test method are as follows:  
1.3.1 Prepared Biomass Samples:  
1.3.1.1 Air Dried (%Tad)—The percent, by mass, of total solids of the air-dried sample.
1.3.1.2 45 °C Dried (%T45)—The percent, by mass, of total solids of the 45 °C dried sample.
1.3.1.3 Freeze Dried (%Tfd)—The percent, by mass, of total solids of the freeze dried sample.  
1.3.2 Extractives-Free Sample (%T ext)—The percent, by mass, of total solids of the extracted sample determined at 105°C.  
1.4 The values stated in SI units are to be regarded as the standard.  
1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. Specific precautionary statements are given in Note 3 and Note 4.  
1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
4.1 Moisture is a ubiquitous and variable component of any biomass sample. Moisture is not considered a structural component of biomass and can change with storage and handling of biomass samples. The determination of the total solids content allows for the correction of biomass samples to an oven-dried solids mass that is constant for a particular sample.  
4.2 This procedure is not suitable for biomass samples that visibly change on heating to 105 °C, for example, unwashed acid-pretreated biomass still containing free acid.  
4.3 Some materials that contain large amount of free sugars or proteins will caramelize or brown under direct infrared heating elements used in Test Method B. Total solids in these materials should be done by Test Method A.
SCOPE
1.1 This test method covers the determination of the amount of total solids remaining after drying a sample. Materials suitable for this procedure include samples prepared in accordance with Practice E1757 and extractive-free material prepared in accordance with Test Method E1690. For particulate wood fuels, Test Method E871 should be used.  
1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SCOPE
1.1 This practice provides guidelines and criteria to follow when selecting reference scenarios, utilizing science-based measurable indicators, to facilitate a transparent and replicable comparison.  
1.1.1 It is a common desire for decision makers, researchers, and others to assess the effects of bioproducts. Such assessments inherently require the comparison of conditions under a system with the bioproduct (test scenario) to a system without the bioproduct (the reference scenario).  
1.1.2 This practice is applicable, but not limited to, life-cycle assessments (LCA), sustainability analyses, and techno-economic assessments (TEA).  
1.2 This practice provides consistent terminology for use with the test and reference scenario. The terminology used in this practice may be used in other documents and by other practitioners with alternate definitions.  
1.3 This practice is applicable whenever the test or reference scenario involves biomass directly or energy or industrial chemicals from biomass.  
1.4 This practice provides guidelines for developing and documenting reference scenarios that represent the best available data and projections for what is expected to occur in the absence of the biomass-based test scenario to be evaluated.  
1.5 The practice is applicable to:  
1.5.1 Reviews and evaluations of the suitability of the reference scenario selected for an existing study or comparison.  
1.5.2 All biomass-based production systems and materials, including forestry, agriculture, algae, co-products, and wastes.  
1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.7 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
5.1 While pyrolysis bio-oils are comprised of a large variety of compounds and chemical functional groups, quantification of carbonyl groups is especially important. Carbonyls are known to be responsible for the instability of bio-oil during both storage and processing. This method can be used to quantify the total carbonyl content of bio-oils.
SCOPE
1.1 This test method covers the determination of the carbonyl content of bio-oils derived from thermochemical decomposition of lignocellulosic biomass and their deoxygenated products. This method is used for determination of carbonyls between 0.5 and 8 mol/kg.  
1.2 Review the current and appropriate Safety Data Sheets (SDS) for detailed information concerning toxicity, first aid procedures, and safety precautions and proper personal protective equipment.  
1.3 Units—The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.5 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SCOPE
1.1 This standard practice provides a science-based methodology for evaluating the relative sustainability of options involving energy or chemicals derived from biomass. Options may involve products, processes, or projects.  
1.2 The methodology includes setting goals and objectives, identifying stakeholders, selecting appropriate indicators, and evaluating the relative sustainability of options where at least one option is available from biomass.  
1.3 The objectives are to facilitate fair comparison of options, focus efforts on practical indicators reflecting stakeholder priorities, and support continual improvement for more sustainable outcomes.  
1.4 The purpose of this standard practice is not to declare something as sustainable or not sustainable but to help users assess, compare, and rank options based on specific goals and objectives.  
1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
4.1 In 2014, the U.S. EPA published the final rules adding renewable fuel pathways under the RFS Program. The rules qualified kernel fiber as a cellulosic feedstock meeting the 60 % greenhouse gas (GHG) reduction and qualifies for the generation of D3 RINs. These rules allow for two approaches for kernel fiber conversion (CFR 40, Part 80 and EPA-HQ-OAR-2012-0401; FRL-9910-40-OAR):  
4.1.1 Producers of cellulosic fuels derived from conversion of feedstocks that are predominantly cellulosic, where “predominantly cellulosic” is defined as feedstock that has an average adjusted cellulosic content of 75 %, measured on a dry mass basis; furthermore, this ‘‘adjusted cellulosic content’’ is the percent of organic (non-ash) material that is cellulose, hemicellulose, or lignin (CFR 40, Part 80 and EPA-HQ-OAR-2012-0401; FRL-9910-40-OAR).  
4.1.2 Producers of cellulosic fuels derived from the simultaneous conversion of feedstocks that are predominantly cellulosic and feedstocks that are not predominantly cellulosic (CFR 40, Part 80 and EPA-HQ-OAR-2012-0401; FRL-9910-40-OAR).  
4.2 Producers that wish to gain approval to the pathway that claims simultaneous conversion of feedstocks that are predominantly cellulosic and feedstocks that are not predominantly cellulosic are required to quantify the amount of renewable fuel that is derived specifically from cellulosic content and from starch. To accomplish this, the producer needs to quantify the amount of cellulosic content and starch present before the conversion process begins and after the conversion process is complete. These measurements of cellulosic content and starch content before and after conversion are used to calculate a converted fraction of each, which is then used to ratio the renewable fuel produced accordingly and assign those respective gallons the D6 or D3 RIN code (CFR 40, Part 80 and EPA-HQ-OAR-2012-0401; FRL-9910-40-OAR).
SCOPE
1.1 This practice provides criteria for the sampling, testing, and calculation methodologies used for the quantification of the converted fraction of starch and cellulosic content. Furthermore, this practice covers procedures for the management of the standard error associated with the sampling and testing of before conversion and after conversion samples from a fuel ethanol production facility.  
1.1.1 This practice can be used to determine the volume of renewable fuel produced from the simultaneous conversion of starch and cellulosic material eligible for generating D3 RINs under the United States (U.S.) Renewable Fuel Standard (RFS).  
1.2 This practice covers the collection and testing of heterogeneous material, including, but not limited to: corn, sorghum, wheat, mash, beer, whole stillage, dried distillers grains with solubles (DDGS), and dried distillers grains.  
1.3 This practice is intended to be used in renewable fuel production facilities designed to produce renewable alcohols. Use of this practice in any other type of process has not been reviewed.  
1.4 This practice can be utilized using either manual or automatic sampling techniques, so long as the criteria of this practice are followed.  
1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SCOPE
1.1 This specification covers biomass derived, undenatured ethanol intended for use in industrial applications such as adhesives, detergents, inks, chemicals, plastics, paints, thinners, etc.  
1.2 Nothing in this specification shall preclude observance of federal, state, or local regulations.  
1.3 Undenatured ethanol has many regulatory limitations that cover the production, trading, transporting, distributing, wholesale and retail sale, and use of undenatured ethanol; this specification does not purport to address the regulatory compliance aspects of these activities.  
1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.5 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
4.1 These test methods of analysis described herein can be used for the proximate analysis, ultimate analysis, and the determination of the gross caloric value of wood fuels.
SCOPE
1.1 These test methods cover the proximate and ultimate analysis of wood fuels and the determination of the gross caloric value of wood fuels sampled and prepared by prescribed test methods and analyzed according to ASTM established procedures. Test methods as herein described may be used to establish the rank of fuels, to show the ratio of combustible to incombustible constituents, to provide the basis for buying and selling, and to evaluate for beneficiation or for other purposes.  
1.2 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.3 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
4.1 This test method provides a rapid determination for moisture in particulate wood fuels in several minutes. The standard method, Test Method E871, requires a minimum of 18 h. This method is applicable to situations such as the spot-check of the moisture delivered by truck where a quick indication of the moisture of wood delivered is desirable.
SCOPE
1.1 This test method provides an alternative method to Test Method E871, for the determination of the moisture of particulate wood fuels. Particulate wood fuels are defined in Terminology E1126.  
1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
4.1 The test procedures described in this test method can be used to determine the percentage of gaseous products, exclusive of moisture vapor, of any particulate wood fuel meeting the requirements specified in this test method.
SCOPE
1.1 This test method determines the percentage of gaseous products, exclusive of moisture vapor, in the analysis sample of particulate wood fuel that is released under the specific conditions of the test. The particulate wood fuel may be sanderdust, sawdust, pellets, green tree chips, hogged fuel, or other type particulate wood fuel having a maximum particle volume of 16.39 cm3  (1 in.3). Volatile matter, when determined as herein described, may be used to indicate yields on processes to provide the basis for purchasing and selling or to establish burning characteristics.  
1.2 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.  
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
4.1 Preparation Method A—Method suitable for the preparation of large quantities (>20 g) of field collected samples into a form appropriate for compositional analysis. Woody samples must first be available as chips of a nominal 5 by 5 by 0.6 cm (2 by 2 by 1/4 in.) or less and twigs not exceeding 0.6 cm (1/4 in.) diameter. Herbaceous materials may be processed as whole straw. It is recommended that wastepaper should be shredded into pieces less then 1 cm (1/2 in.) wide. Furthermore, it is recommended that twigs, straw, and wastepaper should not exceed 61 cm (24 in.) in length to facilitate handling.  
4.2 Preparation Methods B and C—Methods are suitable for very moist feedstocks, samples that would not be stable during prolonged exposure to ambient conditions, or for drying materials when room conditions deviate from the ambient conditions described in 3.1.1. These test methods are also suitable for handling small samples of biomass (  
4.3 Preparation Methods A, B, and C are not intended for materials that will already pass through a 20 mesh sieve or that cannot be dried by the described methods to a total solids content of greater than 85 %, based on an oven dried weight.  
4.4 Preparation Method A will separate the milled material into two fractions, a −20/+80 mesh fraction and a −80 mesh fraction.  
4.4.1 Extraneous inorganic materials will accumulate in the −80 mesh fraction and it should be analyzed independently from the −20/+80 mesh fraction. Weighted results from the two fractions can then be combined to obtain results for materials on an "as received" basis.
Note 1: During analysis, the very fine consistency of the −80 mesh fraction may cause problems in filtering operations and should be handled appropriately.  
4.5 Preparation Method D—Method suitable for cereal grains, cereal grain fermentation mash, cereal grain fermentation beer, and cereal grain fermentation residues that are generally stable.  
4.6 Preparation Method E—Method suitable for ce...
SCOPE
1.1 This practice covers a reproducible way to convert hardwoods, softwoods, herbaceous materials (such as switchgrass and sericea), agricultural residues (such as corn stover, wheat straw, and bagasse), wastepaper (such as office waste, boxboard, and newsprint), cellulosic feedstocks pretreated to improve suitability for fermentation, cereal grains, cereal grain fermentation mash, cereal grain fermentation beer, and fermentation residues into a uniform material suitable for compositional analysis. This practice is intended for samples that need to be dried prior to analysis.  
1.2 Milling and sieving actions both produce large amounts of dust. This dust can be a nuisance hazard and irritant. Use appropriate respiratory protection as needed. If excessive amounts of dust are allowed to become airborne a potential explosion hazard is possible. Provide appropriate dust control measures as needed.  
1.3 The values stated in SI units are to be regarded as standard. The values given in parentheses after SI units are provided for information only and are not considered standard.  
1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.5 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
2.1 The test procedures described in this test method can be used to determine the bulk density (or bulk specific weight) of any densified particulate biomass fuel meeting the requirements specified in this test method.
SCOPE
1.1 This test method covers the procedure for the determination of bulk density (or bulk specific weight) of densified particulate biomass fuels with a maximum particle volume of 16.39 cm3  (1 in. 3).  
1.2 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.  
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
5.1 The ash content determines the weight of the noncombustible part of a particulate wood fuel that oxidizes during a combustion process but releases no energy.
SCOPE
1.1 This test method covers the determination of ash expressed as the percent of residue remaining after dry oxidation of particulate wood fuels. Particulate wood fuels are defined in Terminology E1126.  
1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
4.1 The test procedures described in this test method can be used to determine the total weight basis moisture of any particulate wood fuel meeting the requirements specified in this test method.
SCOPE
1.1 This test method covers the determination of total weight basis moisture in the analysis sample of particulate wood fuel. The particulate wood fuel may be sanderdust, sawdust, pellets, green tree chips, hogged fuel, or other type particulate wood fuel having a maximum particle volume of 16.39 cm3  (1 in.3). It is used for calculating other analytical results to a dry basis. Moisture, when determined as herein described, may be used to indicate yields on processes, to provide the basis for purchasing and selling, or to establish burning characteristics.  
1.2 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.  
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SCOPE
1.1 This document is composed of terms, definitions of terms, descriptions of terms, and acronyms used in ASTM documents related to the field of biotechnology. Terms that are adequately defined in a general dictionary are not defined in this terminology standard.  
1.2 This standard includes terminology used in biotechnology areas, such as, but not limited to: biological drug products, materials for biotechnology, characterization and identification of biological systems, aseptic sampling, preservation of biological samples, membrane filters, molecular biology, biomass conversion, fuel manufacturing facilities, and fuel analysis.  
1.3 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SCOPE
1.1 This specification covers denatured ethanol intended to be used as a cooking or appliance fuel, or both.  
1.1.1 This product is intentionally denatured as an additional deterrent to consumption and to avoid beverage alcohol use.  
1.1.2 This specification allows for various denaturants to be used for the intended purpose of cooking or appliance fuel. Jurisdictions may vary in their regulatory requirement for the allowable or prohibited types of denaturants, chemical composition of the denaturant or concentration of denaturant needed to denature the ethanol.  
1.2 Nothing in this specification shall preclude observance of federal, state, or local regulations.  
1.3 Denatured ethanol has many regulatory limitations that cover the production, trading, transporting, distributing, wholesale and retail sale, and use of denatured ethanol; this specification does not purport to address the regulatory compliance aspects of these activities.  
1.4 The application and intent of this product is for household cooking fuel and not for on-road motor fuel use (see D4806).  
1.5 Units—The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.7 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
5.1 While pyrolysis bio-oils are comprised of a large variety of compounds and chemical functional groups, quantification of carbonyl groups is especially important. Carbonyls are known to be responsible for the instability of bio-oil during both storage and processing. This method can be used to quantify the total carbonyl content of bio-oils.
SCOPE
1.1 This test method covers the determination of the carbonyl content of bio-oils derived from thermochemical decomposition of lignocellulosic biomass and their deoxygenated products. This method is used for determination of carbonyls between 0.5 and 8 mol/kg.  
1.2 Review the current and appropriate Safety Data Sheets (SDS) for detailed information concerning toxicity, first aid procedures, and safety precautions and proper personal protective equipment.  
1.3 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.5 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
5.1 While pyrolysis bio-oils are comprised of a large variety of compounds and chemical functional groups, quantification of carbonyl groups is especially important. Carbonyls are known to be responsible for the instability of bio-oil during both storage and processing. This method can be used to quantify the total carbonyl content of bio-oils.
SCOPE
1.1 This test method covers the determination of the carbonyl content of bio-oils derived from thermochemical decomposition of lignocellulosic biomass and their deoxygenated products. This method is used for determination of carbonyls between 0.5 and 8 mol/kg.  
1.2 Review the current and appropriate Safety Data Sheets (SDS) for detailed information concerning toxicity, first aid procedures, and safety precautions and proper personal protective equipment.  
1.3 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.5 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SCOPE
1.1 This standard practice provides a science-based methodology for evaluating the relative sustainability of options involving energy or chemicals derived from biomass. Options may involve products, processes, or projects.  
1.2 The methodology includes setting goals and objectives, identifying stakeholders, selecting appropriate indicators, and evaluating the relative sustainability of options where at least one option is available from biomass.  
1.3 The objectives are to facilitate fair comparison of options, focus efforts on practical indicators reflecting stakeholder priorities, and support continual improvement for more sustainable outcomes.  
1.4 The purpose of this standard practice is not to declare something as sustainable or not sustainable but to help users assess, compare, and rank options based on specific goals and objectives.  
1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

SCOPE
1.1 This practice provides a methodology for assessing the relative sustainability of products, processes or projects involving energy and chemicals derived from biomass.  
1.2 These assessments will facilitate informed decisions, leading to more sustainable outcomes.  
1.3 This practice provides a methodology that includes setting goals and objectives, identifying the appropriate context and selecting appropriate indicators that ensure comparable, equivalent and replicable assessments of relative sustainability where at least one option is available from biomass.  
1.4 The objectives of this practice are to reduce bias and distortion in the marketplace, facilitate fair comparison of options, focus on assessments reflecting stakeholder priorities, and support continual improvement for more sustainable outcomes.  
1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

SIGNIFICANCE AND USE
4.1 Ethanol extractives are any materials found in biomass that are soluble in ethanol. They are not considered to be part of the structural components of biomass and should be removed prior to any chemical analysis of the sample. The prolonged extraction removes nonstructural materials that can include waxes, fats, resins, tannins, gums, sugars, starches, and pigments. Removing hydrophobic materials from the biomass makes it easier to wet the material for the analysis of structural components in the biomass.  
4.2 This test method yields results comparable to Test Method D1107.
SCOPE
1.1 This test method covers the determination of ethanol soluble extractives, expressed as a percentage of the oven-dried biomass, of hard and soft woods, herbaceous materials (such as switchgrass and sericea), agricultural residues (such as corn stover, wheat straw, and bagasse), and wastepaper (such as office waste, boxboard, and newsprint). All results are reported relative to the oven-dried weight of the sample.
Note 1: This test method may not be suitable for hard and soft wood samples that include bark or those with more than 10% extractable materials.  
1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

ABSTRACT
This specification provides requirements and corresponding test methods for denatured ethanol intended to be used as a cooking or appliance fuel, or both. The addition of a denatonium benzoate and a colored dye is required. The minimum and maximum requirements for the amount in which denatonium benzoate must be added are specified. The hydrocarbons approved for use under this specification are gasoline, unleaded gasoline, natural gasoline, heptane, or rubber hydrocarbon solvent.
Also addressed in this specification are workmanship, sampling, sontainers, and sample handling. The test methods cover ethanol, water, and higher alcohols as well as acidity and solvent-washed gum content.
SCOPE
1.1 This specification covers denatured ethanol intended to be used as a cooking or appliance fuel, or both.  
1.2 Nothing in this specification shall preclude observance of federal, state, or local regulations.  
1.2.1 Denatured ethanol has many regulatory limitations that cover the production, trading, transporting, distributing, wholesale and retail sale, and use of denatured ethanol; this specification does not purport to address the regulatory compliance aspects of these activities.  
1.3 The addition of a denatonium benzoate is required in addition to any other denaturants that are added.  
1.3.1 The denatonium benzoate is added as an additional deterrent to consumption. The denatonium benzoate must be added in an amount that meets the minimum and maximum requirements listed in Table 1.  
1.4 The addition of a colored dye is also required. The colored dye must be added in a concentration sufficient to enable the visual detection of the color in the fuel but in an amount less than the maximum listed in Table 1.  
1.4.1 The colored dye is added to visually indicate that the product is not potable.  
1.5 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

SIGNIFICANCE AND USE
4.1 Preparation Method A—Method suitable for the preparation of large quantities (>20 g) of field collected samples into a form appropriate for compositional analysis. Woody samples must first be available as chips of a nominal 5 by 5 by 0.6 cm (2 by 2 by 1/4 in.) or less and twigs not exceeding 0.6 cm (1/4 in.) diameter. Herbaceous materials may be processed as whole straw. It is recommended that wastepaper should be shredded into pieces less then 1 cm (1/2 in.) wide. Furthermore, it is recommended that twigs, straw and wastepaper should not exceed 61 cm (24 in.) in length to facilitate handling.  
4.2 Preparation Methods B and C—Test methods are suitable for very moist feedstocks, samples that would not be stable during prolonged exposure to ambient conditions, or for drying materials when room conditions deviate from the ambient conditions described in 3.1.1. These test methods are also suitable for handling small samples of biomass (  
4.3 This practice is not intended for materials that will already pass through a 20 mesh sieve or cannot be dried by the described methods to a total solids content of greater then 85 %, based on an oven dried weight.  
4.4 This practice will separate the milled material into two fractions, a −20/+80 mesh fraction and a −80 mesh fraction.  
4.4.1 Extraneous inorganic materials will accumulate in the -80 mesh fraction and it should be analyzed independently from the -20/+80 mesh fraction. Weighted results from the two fractions can then be combined to obtain results for materials on an "as received" basis.
Note 1: During analysis, the very fine consistency of the -80 mesh fraction may cause problems in filtering operations and should be handled appropriately.
SCOPE
1.1 This practice covers a reproducible way to convert hardwoods, softwoods, herbaceous materials (such as switchgrass and sericea), agricultural residues (such as corn stover, wheat straw, and bagasse), wastepaper (such as office waste, boxboard, and newsprint), feedstocks pretreated to improve suitability for fermentation and fermentation residues into a uniform material suitable for compositional analysis.  
1.2 Milling and sieving actions both produce large amounts of dust. This dust can be a nuisance hazard and irritant. Use appropriate respiratory protection as needed. If excessive amounts of dust are allowed to become airborne a potential explosion hazard is possible. Provide appropriate dust control measures as needed.  
1.3 The values stated in SI units are to be regarded as the standard. The inch-pound units given in parentheses are for information only.  
1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

SIGNIFICANCE AND USE
4.1 The ash content is an approximate measure of the mineral content and other inorganic matter in biomass.  
4.2 The ash content is used in conjunction with other assays to determine the total composition of biomass samples.
SCOPE
1.1 This test method covers the determination of ash, expressed as the mass percent of residue remaining after dry oxidation (oxidation at 575 ± 25°C), of hard and soft woods, herbaceous materials (such as switchgrass and sericea), agricultural residues (such as corn stover, wheat straw, and bagasse), wastepaper (such as office waste, boxboard, and newsprint), acid and alkaline pretreated biomass, and the solid fraction of fermentation residues. All results are reported relative to the 105°C oven-dried mass of the sample. For particulate wood fuels, Test Method E1534 should be used.  
1.2 The values stated in SI units are to be regarded as the standard.  
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

SCOPE
1.1 This document is composed of terms, definitions of terms, descriptions of terms, and acronyms used in ASTM documents related to the field of biotechnology. Terms that are adequately defined in a general dictionary are not defined in this terminology standard.  
1.2 This standard includes terminology used in biotechnology areas, such as, but not limited to: biological drug products, materials for biotechnology, characterization and identification of biological systems, aseptic sampling, preservation of biological samples, membrane filters, molecular biology, biomass conversion, fuel manufacturing facilities, and fuel analysis.

SIGNIFICANCE AND USE
4.1 Moisture is a ubiquitous and variable component of any biomass sample. Moisture is not considered a structural component of biomass and can change with storage and handling of biomass samples. The determination of the total solids content allows for the correction of biomass samples to an oven-dried solids mass that is constant for a particular sample.  
4.2 This procedure is not suitable for biomass samples that visibly change on heating to 105°C, for example, unwashed acid-pretreated biomass still containing free acid.  
4.3 Some materials that contain large amount of free sugars or proteins will caramelize or brown under direct infrared heating elements used in Test Method B. Total solids in these materials should be done by Test Method A.
SCOPE
1.1 This test method covers the determination of the amount of total solids remaining after drying a sample. Materials suitable for this procedure include samples prepared in accordance with Practice E1757 and extractive-free material prepared in accordance with Test Method E1690. For particulate wood fuels, Test Method E871 should be used.  
1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

SIGNIFICANCE AND USE
4.1 The structural carbohydrate content is used in conjunction with other assays to determine the total composition of biomass samples.
SCOPE
1.1 This test method describes the determination of structural carbohydrates present in a biomass sample, expressed as the percent mass of an oven-dried sample basis of each anhydrosugar.  
1.2 Sample materials suitable for this procedure include hard and softwoods, herbaceous materials, such as sericea and switchgrass, agricultural residues, such as corn stover, wheat straw, and bagasse, wastepaper, such as boxboard, office waste, and newsprint, acid or alkaline-pretreated biomass, washed free of any residual acid or alkali, and the solid fraction of fermentation residues.  
1.3 The options for the types of samples to be analyzed in this procedure are:  
1.3.1 Prepared Biomass Samples:  
1.3.1.1 Air Dried Material—Results are reported as the percent by mass, based on the oven-dried mass of the air-dried sample.
1.3.1.2 45°C Dried Material—Results are reported as the percent by mass, based on the oven-dried mass of the 45°C dried sample.
1.3.1.3 Freeze Dried Material—Results are reported as the percent by mass, based on the oven-dried mass of the freeze dried sample.  
1.3.2 Extractives-Free Sample—Results are reported as the percent by mass, based on the oven-dried mass of the extracted sample.  
1.4 This standard method is generally not suitable for samples that contain soluble, nonstructural carbohydrates unless they are removed prior to analysis.  
1.5 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.  See Section 8 for specific hazards statements.

SIGNIFICANCE AND USE
4.1 The percentage, by mass, of sugar content is used in conjunction with other assays to determine the total composition of biomass samples.
SCOPE
1.1 This test method covers the determination of carbohydrates present in a biomass sample, expressed as the percent, by mass, of each sugar on a 105°C dried mass basis.
Note 1: The percent sugar must be corrected for the water of hydrolysis before calculating the actual mass percent of the polysaccharide in the original biomass sample.  
1.2 Sample materials suitable for this procedure include hard and soft woods, herbaceous materials (such as switchgrass and sericea), agricultural residues (such as corn stover, wheat straw, and bagasse), wastepaper (such as office waste, boxboard, and newsprint), acid or alkaline-pretreated biomass (washed free of any residual acid or alkali), and the solid fraction of fermentation residues. All results are reported relative to the 105°C oven-dried mass of the sample.  
1.3 The options for the types of samples to be analyzed in this test method are as follows:  
1.3.1 Prepared Biomass Samples:  
1.3.1.1 Air Dried (%Tad)—The percent, by mass, of total solids of the air-dried sample.
1.3.1.2 45°C Dried (%T45)—The percent, by mass, of total solids of the 45°C dried sample.
1.3.1.3 Freeze Dried (%Tfd)—The percent, by mass, of total solids of the freeze dried sample.  
1.3.2 Extractives-Free Sample (%T ext)—The percent, by mass, of total solids of the extracted sample determined at 105°C.  
1.4 The values stated in SI units are to be regarded as the standard.  
1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. Specific precautionary statements are given in Note 3 and Note 4.

SIGNIFICANCE AND USE
4.1 The acid-insoluble residue content is used in conjunction with other assays to determine the total composition of biomass samples.
SCOPE
1.1 This test method covers determination of the acid-insoluble residue of hard and soft woods, herbaceous materials (such as switchgrass and sericea), agricultural residues (such as corn stover, wheat straw, and bagasse), wastepaper (such as office waste, boxboard, and newsprint), acid and alkaline pretreated biomass, and the solid fraction of fermentation residues. All results are reported relative to the 105°C oven-dried weight of the sample.  
1.2 The residue collected contains the acid-insoluble lignin and any condensed proteins from the original sample. An independent nitrogen analysis would be required to determine the acid-insoluble lignin content separate from the condensed protein fraction and is outside the scope of this test method.  
1.3 A portion of the lignin in some biomass samples will remain soluble during this procedure. The total lignin in a biomass sample includes both acid-soluble lignin and lignin in the acid insoluble residue.  
1.4 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. Specific hazards statements are given in Section 8 and Note 2 and Note 4.

SCOPE
1.1 This document is composed of terms, definitions of terms, descriptions of terms, and acronyms used in ASTM documents related to the field of biotechnology. Terms that are adequately defined in a general dictionary are not defined in this terminology standard.  
1.2 This standard includes terminology used in biotechnology areas, such as, but not limited to: biological drug products, materials for biotechnology, characterization and identification of biological systems, aseptic sampling, preservation of biological samples, membrane filters, molecular biology, biomass conversion, fuel manufacturing facilities, and fuel analysis.

SIGNIFICANCE AND USE
4.1 The test procedures described in this test method can be used to determine the total weight basis moisture of any particulate wood fuel meeting the requirements specified in this test method.
SCOPE
1.1 This test method covers the determination of total weight basis moisture in the analysis sample of particulate wood fuel. The particulate wood fuel may be sanderdust, sawdust, pellets, green tree chips, hogged fuel, or other type particulate wood fuel having a maximum particle volume of 16.39 cm3  (1 in.3). It is used for calculating other analytical results to a dry basis. Moisture, when determined as herein described, may be used to indicate yields on processes, to provide the basis for purchasing and selling, or to establish burning characteristics.  
1.2 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.  
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

SIGNIFICANCE AND USE
4.1 The test procedures described in this test method can be used to determine the percentage of gaseous products, exclusive of moisture vapor, of any particulate wood fuel meeting the requirements specified in this test method.
SCOPE
1.1 This test method determines the percentage of gaseous products, exclusive of moisture vapor, in the analysis sample of particulate wood fuel that is released under the specific conditions of the test. The particulate wood fuel may be sanderdust, sawdust, pellets, green tree chips, hogged fuel, or other type particulate wood fuel having a maximum particle volume of 16.39 cm3  (1 in.3). Volatile matter, when determined as herein described, may be used to indicate yields on processes to provide the basis for purchasing and selling or to establish burning characteristics.  
1.2 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.  
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

SIGNIFICANCE AND USE
5.1 The ash content determines the weight of the noncombustible part of a particulate wood fuel that oxidizes during a combustion process but releases no energy.
SCOPE
1.1 This test method covers the determination of ash expressed as the percent of residue remaining after dry oxidation of particulate wood fuels. Particulate wood fuels are defined in Terminology E1126.  
1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

SIGNIFICANCE AND USE
2.1 The test procedures described in this test method can be used to determine the bulk density (or bulk specific weight) of any densified particulate biomass fuel meeting the requirements specified in this test method.
SCOPE
1.1 This test method covers the procedure for the determination of bulk density (or bulk specific weight) of densified particulate biomass fuels with a maximum particle volume of 16.39 cm3  (1 in. 3).  
1.2 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.  
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

SIGNIFICANCE AND USE
4.1 These test methods of analysis described herein can be used for the proximate analysis, ultimate analysis, and the determination of the gross caloric value of wood fuels.
SCOPE
1.1 These test methods cover the proximate and ultimate analysis of wood fuels and the determination of the gross caloric value of wood fuels sampled and prepared by prescribed test methods and analyzed according to ASTM established procedures. Test methods as herein described may be used to establish the rank of fuels, to show the ratio of combustible to incombustible constituents, to provide the basis for buying and selling, and to evaluate for beneficiation or for other purposes.  
1.2 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

SIGNIFICANCE AND USE
4.1 This test method provides a rapid determination for moisture in particulate wood fuels in several minutes. The standard method, E871, requires a minimum of 18 h. This method is applicable to situations such as the spot-check of the moisture delivered by truck where a quick indication of the moisture of wood delivered is desirable.
SCOPE
1.1 This test method provides an alternative method to Method E871, for the determination of the moisture of particulate wood fuels. Particulate wood fuels are defined in E1126.  
1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.  
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

SCOPE
1.1 This document is composed of terms, definitions of terms, descriptions of terms, and acronyms used in ASTM documents related to the field of biotechnology. Terms that are adequately defined in a general dictionary are not defined in this terminology standard.
1.2 This standard includes terminology used in biotechnology areas, such as, but not limited to: biological drug products, materials for biotechnology, characterization and identification of biological systems, aseptic sampling, preservation of biological samples, membrane filters, molecular biology, biomass conversion, fuel manufacturing facilities, and fuel analysis.

SCOPE
1.1 This document is composed of terms, definitions of terms, descriptions of terms, and acronyms used in ASTM documents related to the field of biotechnology. Terms that are adequately defined in a general dictionary are not defined in this terminology standard.  
1.2 This standard includes terminology used in biotechnology areas, such as, but not limited to: biological drug products, materials for biotechnology, characterization and identification of biological systems, aseptic sampling, preservation of biological samples, membrane filters, molecular biology, biomass conversion, fuel manufacturing facilities, and fuel analysis.
WITHDRAWN RATIONALE
This document is composed of terms, definitions of terms, descriptions of terms, and acronyms used in ASTM documents related to the field of biotechnology. Terms that are adequately defined in a general dictionary are not defined in this terminology standard.
Formerly under the jurisdiction of Committee E48 on Boitechnology, this terminology was withdrawn in January 2011 in accordance with section 10.5.3.1 of the Regulations Governing ASTM Technical Committees, which requires that standards shall be updated by the end of the eighth year since the last approval date.

SIGNIFICANCE AND USE
The acid-insoluble residue content is used in conjunction with other assays to determine the total composition of biomass samples.
SCOPE
1.1 This test method covers determination of the acid-insoluble residue of hard and soft woods, herbaceous materials (such as switchgrass and sericea), agricultural residues (such as corn stover, wheat straw, and bagasse), wastepaper (such as office waste, boxboard, and newsprint), acid and alkaline pretreated biomass, and the solid fraction of fermentation residues. All results are reported relative to the 105°C oven-dried weight of the sample.
1.2 The residue collected contains the acid-insoluble lignin and any condensed proteins from the original sample. An independent nitrogen analysis would be required to determine the acid-insoluble lignin content separate from the condensed protein fraction and is outside the scope of this test method.
1.3 A portion of the lignin in some biomass samples will remain soluble during this procedure. The total lignin in a biomass sample includes both acid-soluble lignin and lignin in the acid insoluble residue.
1.4 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. Specific hazards statements are given in Section 8 and Note 2 and Note 4.

SIGNIFICANCE AND USE
The structural carbohydrate content is used in conjunction with other assays to determine the total composition of biomass samples.
SCOPE
1.1 This test method describes the determination of structural carbohydrates present in a biomass sample, expressed as the percent mass of an oven-dried sample basis of each anhydrosugar.  
1.2 Sample materials suitable for this procedure include hard and softwoods, herbaceous materials, such as sericea and switchgrass, agricultural residues, such as corn stover, wheat straw, and bagasse, wastepaper, such as boxboard, office waste, and newsprint, acid or alkaline-pretreated biomass, washed free of any residual acid or alkali, and the solid fraction of fermentation residues.
1.3 The options for the types of samples to be analyzed in this procedure are:
1.3.1 Prepared Biomass Samples:  
1.3.1.1 Air Dried Material—Results are reported as the percent by mass, based on the oven-dried mass of the air-dried sample.
1.3.1.2 45°C Dried Material—Results are reported as the percent by mass, based on the oven-dried mass of the 45°C dried sample.
1.3.1.3 Freeze Dried Material—Results are reported as the percent by mass, based on the oven-dried mass of the freeze dried sample.
1.3.2 Extractives-Free Sample—Results are reported as the percent by mass, based on the oven-dried mass of the extracted sample.
1.4 This standard method is generally not suitable for samples that contain soluble, nonstructural carbohydrates unless they are removed prior to analysis.
1.5 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.  See Section 8 for specific hazards statements.

SIGNIFICANCE AND USE
Ethanol extractives are any materials found in biomass that are soluble in ethanol. They are not considered to be part of the structural components of biomass and should be removed prior to any chemical analysis of the sample. The prolonged extraction removes nonstructural materials that can include waxes, fats, resins, tannins, gums, sugars, starches, and pigments. Removing hydrophobic materials from the biomass makes it easier to wet the material for the analysis of structural components in the biomass.
This test method yields results comparable to Test Method D 1107.
SCOPE
1.1 This test method covers the determination of ethanol soluble extractives, expressed as a percentage of the oven-dried biomass, of hard and soft woods, herbaceous materials (such as switchgrass and sericea), agricultural residues (such as corn stover, wheat straw, and bagasse), and wastepaper (such as office waste, boxboard, and newsprint). All results are reported relative to the oven-dried weight of the sample.
Note 1—This test method may not be suitable for hard and soft wood samples that include bark or those with more than 10% extractable materials.
1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.