EN ISO 18744:2016/prA1:2025

SLOVENSKI STANDARD
01-maj-2025
Mikrobiologija v prehranski verigi - Ugotavljanje prisotnosti in števila
"Cryptosporidium" in "Giardia" v sveži zeleni listni zelenjavi in jagodičevju -
Dopolnilo A1: Študija validacije metode in značilnosti delovanja (ISO
18744:2016/DAmd 1:2025)
Microbiology of the food chain - Detection and enumeration of Cryptosporidium and
Giardia in fresh leafy green vegetables and berry fruits - Amendment 1: Method
validation studies and performance characteristics (ISO 18744:2016/DAmd 1:2025)
Mikrobiologie der Lebensmittelkette - Nachweis und Zählung von Cryptosporidium und
Giardia in frischem grünem Blattgemüse und Beeren -Änderung 1 (ISO
18744:2016/DAmd 1:2025)
Microbiologie de la chaîne alimentaire - Recherche et dénombrement de
Cryptosporidium et Giardia dans les légumes verts frais à feuilles et les fruits à baies -
Amendement 1: Études de validation des méthodes et caractéristiques de performance
(ISO 18744:2016/DAmd1:2025)
Ta slovenski standard je istoveten z: EN ISO 18744:2016/prA1:2025
ICS:
07.100.30 Mikrobiologija živil Food microbiology
67.080.01 Sadje, zelenjava in njuni Fruits, vegetables and
proizvodi na splošno derived products in general
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

DRAFT
Amendment
ISO 18744:2016/
DAM 1
ISO/TC 34/SC 9
Microbiology of the food chain —
Secretariat: AFNOR
Detection and enumeration of
Cryptosporidium and Giardia in
Voting begins on:
2025-03-25
fresh leafy green vegetables and
Voting terminates on:
berry fruits
2025-06-17
AMENDMENT 1: Method validation
studies and performance
characteristics
Microbiologie de la chaîne alimentaire — Recherche et
dénombrement de Cryptosporidium et Giardia dans les légumes
verts frais à feuilles et les fruits à baies
AMENDEMENT 1: Études de validation des méthodes et
caractéristiques de performance
ICS: 07.100.30
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Reference number
ISO 18744:2016/DAM 1:2025(en)
ISO 18744:2016/DAM 1:2025(en)
© ISO 2025
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ii
ISO 18744:2016/DAM 1:2025(en)
Foreword
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This document was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC 9,
Microbiology.
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Title
Delete the text:
and enumeration
Explanation note: WG6 agreed to remove the term enumeration in the title and text since, due to the nature of
the validation, the method cannot be considered quantitative but qualitative and the results should be reported
only as presence/absence of the parasite in the matrix. Any enumeration in the method refers to the number of
pre-labelled and enumerated non-viable Cryptosporidium oocysts and Giardia cysts that may be used as internal
controls. The use of internal controls is recommended not mandatory.
Replace the text with the following:
Microbiology of the food chain — Detection of Cryptosporidium and Giardia on fresh leafy green vegetables
and berry fruits
Contents
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iii
ISO 18744:2016/DAM 1:2025(en)
Annex D (informative) Method validation studies and performance characteristics

Introduction
Line 13
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and for their enumeration
1  Scope
Line 1
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and enumeration
8.1
e)
NOTE 1
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Centrifugation at 2 500 x g can result in a very compact pellet. A lower speed of 1 100 x g for 10 min with no
braking has been reported to give at least equivalent recoveries of Cryptosporidium oocysts and Giardia cysts.
i)
Replace the text with the following:
Centrifuge the eluate at 2 500 x g for 10 min with no braking.
NOTE 2
Replace the text with the following:
Centrifugation at 2 500 x g can result in a very compact pellet. A lower speed of 1 100 x g for 10 min with no
braking has been reported to give at least equivalent recoveries of Cryptosporidium oocysts and Giardia cysts.

8.2
g)
Replace the text with the following:
Centrifuge the eluate at 2 500 x g for 10 min with no braking.

NOTE 2
Replace the text with the following:
Centrifugation at 2 500 x g can result in a very compact pellet. A lower speed of 1 100 x g for 10 min with no
braking has been reported to give at least equivalent recoveries of Cryptosporidium oocysts and Giardia cysts.

iv
ISO 18744:2016/DAM 1:2025(en)
k)
Replace the text with the following:
Centrifuge the eluate at 2 500 x g for 10 min with no braking.

NOTE 3
Replace the text with the following:
Centrifugation at 2 500 x g can result in a very compact pellet. A lower speed of 1 100 x g for 10 min with no
braking has been reported to give at least equivalent recoveries of Cryptosporidium oocysts and Giardia cysts.

8.5.2.2
NOTE
Third sentence
Replace the text with the following:
Some Cryptosporidium species have diameters up to 8 μm (e.g., C. andersoni and C. muris, which could be
associated with infections in immunocompromised persons in rare cases).

10.1
First line
Replace the text with the following: The result should be given as the presence or absence of Cryptosporidium
oocysts and/or Giardia cysts detected per weight of fresh produce sample examined.

10.2
i)
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The presence or absence of Cryptosporidium oocysts and Giardia cysts, and any relevant details regarding
their identification
...