70/373/EC - Council Directive 70/373/EEC of 20 July 1970 on the introduction of Community methods of sampling and analysis for the official control of feeding-stuffs
Council Directive 70/373/EEC, adopted on 20 July 1970, mandates the introduction of uniform Community methods for sampling and analysis in official controls of feeding-stuffs. Recognizing the importance of feeding-stuffs in agricultural productivity and the need for consistent quality and composition standards, the directive requires Member States to apply harmonized procedures for sampling and analysis to ensure compliance with legal requirements. The directive establishes a procedure whereby these methods are developed, improved, and updated through cooperation between Member States and the European Commission within a Standing Committee for Feeding-stuffs. This collaborative process allows for scientific and technical considerations to guide the creation of standardized methods. Member States must adopt the necessary legal, regulatory, or administrative measures to implement these standards within one year of notification. The directive aims to facilitate the functioning of the common market by harmonizing testing standards, thereby ensuring fairness and uniformity across the Community in the official control of feeding-stuffs.
Purpose
The directive 70/373/EEC was adopted to introduce standardized Community methods for sampling and analysis to be used by official authorities in Member States for the control of feeding-stuffs. Given the critical importance of feeding-stuffs in animal production and agriculture, harmonizing these methods is essential to ensure compliance with quality and composition requirements across the European Economic Community. This uniform approach aims to facilitate the functioning of the common market by eliminating disparities in national methods that could affect feedstuff regulation and trade. It also calls for continuous improvement and adaptation of these methods based on scientific and technical advances.
Key Obligations
- Official Controls: Member States must ensure that official controls on feeding-stuffs are carried out using the Community-established sampling and analysis methods.
- Harmonization: The directive requires all Member States to adopt uniform analytical and sampling procedures for feeding-stuffs controls to ensure consistency in enforcement and compliance assessment.
- Development and Adoption of Methods: The Community methods must be established through directives based on scientific knowledge and proven techniques, with appropriate time limits for national implementation.
- Committee Involvement: A Standing Committee for Feeding-stuffs is tasked with close cooperation between Member States and the Commission, advising on drafting and adopting these methods through a defined procedure involving votes and qualified majorities.
- Implementation: Within one year of notification, Member States are obligated to adopt by law, regulation, or administrative action the provisions necessary to comply with the directive.
Affected Products and Actors
- Products: All feeding-stuffs used for animal agriculture within the Community are directly affected, covering their quality and composition.
- Actors:
- Member State Authorities responsible for official control and enforcement of feeding-stuffs regulations.
- European Commission, which drafts and proposes the technical measures for the methods of sampling and analysis.
- Standing Committee for Feeding-stuffs, which supports cooperation, review, and coordination between the Commission and Member States.
- Producers, marketers, and users of feeding-stuffs, who are indirectly impacted by the harmonized standards and controls ensuring product quality and safety.
Implementation Timeline
- The directive came into force on 20 July 1970.
- Member States were required to bring into force the necessary national provisions within one year following notification.
- The procedure for adopting Community methods involves a committee process that can last up to eighteen months, allowing time for drafting, review, and adoption of technical sampling and analysis methods.
- The directive establishes continuing obligations for updating and improving the harmonized methods via the committee to keep pace with scientific developments.
In summary, Directive 70/373/EEC mandates a harmonized framework for official sampling and analysis of feeding-stuffs across the European Economic Community to support agricultural productivity, market functioning, and consumer protection.
This directive applies to the official control of feeding-stuffs within the European Economic Community. It covers the production, marketing, and use of feeding-stuffs, which are essential for agricultural animal production. The directive mandates the use of uniform Community methods of sampling and analysis by Member States' authorities to verify compliance with legal, regulatory, or administrative requirements regarding the quality and composition of feeding-stuffs. It harmonizes official sampling and analysis methods across Member States to facilitate the common market and ensure consistent control standards for feeding-stuffs. The scope specifically targets activities related to official controls aimed at checking feeding-stuffs used in agriculture, emphasizing maintaining good quality feed for animal production.
Die Richtlinie 70/373/EWG des Rates vom 20. Juli 1970 legt einheitliche Gemeinschaftsverfahren für die Probenahme und Analyse von Futtermitteln zu amtlichen Untersuchungen fest. Ziel ist es, sicherzustellen, dass die Qualität und Zusammensetzung von Futtermitteln innerhalb der Europäischen Wirtschaftsgemeinschaft einheitlich überprüft wird. Dies soll die einheitliche Anwendung von Rechts- und Verwaltungsvorschriften in allen Mitgliedstaaten gewährleisten und so das Funktionieren des Gemeinsamen Marktes fördern. Dabei werden die Verfahren wissenschaftlich fundiert und technisch einheitlich entwickelt und in enger Zusammenarbeit zwischen den Mitgliedstaaten und der Kommission durch einen Ständigen Futtermittelausschuss festgelegt. Die Mitgliedstaaten sind verpflichtet, innerhalb eines Jahres nach Veröffentlichung der Richtlinie die erforderlichen Rechtsakte zu erlassen, um deren Vorgaben zu erfüllen, und die Kommission darüber zu informieren. Die Richtlinie schafft somit eine technische Grundlage für harmonisierte amtliche Kontrollen und trägt zur Steigerung der Qualitätssicherung im Futtermittelbereich innerhalb der Gemeinschaft bei.
Zweck
Die Richtlinie 70/373/EG hat zum Ziel, die Einführung gemeinschaftlicher Probenahmeverfahren und Analysemethoden für die amtliche Untersuchung von Futtermitteln in der Europäischen Wirtschaftsgemeinschaft (EWG) zu regeln. Da die tierische Erzeugung in der Landwirtschaft von der Verwendung geeigneter Futtermittel abhängt, ist die Festlegung einheitlicher und harmonisierter Prüfverfahren in der gesamten Gemeinschaft essenziell, um die Qualität und Zusammensetzung der Futtermittel sicherzustellen und somit die Produktivität der Landwirtschaft zu steigern.
Die Richtlinie dient insbesondere der Harmonisierung der amtlichen Kontrollen von Futtermitteln bei den Mitgliedstaaten, um Abweichungen und Probleme bei der Umsetzung einzelstaatlicher Normen zu vermeiden und den einheitlichen Binnenmarkt zu stärken.
Wesentliche Verpflichtungen
Die Mitgliedstaaten müssen geeignete Maßnahmen treffen, damit amtliche Untersuchungen von Futtermitteln auf Einhaltung der Rechts- und Verwaltungsvorschriften hinsichtlich Beschaffenheit und Zusammensetzung der Futtermittel nach gemeinsamen, in der Gemeinschaft festgelegten Probenahmeverfahren und Analysemethoden erfolgen.
Die Verfahren und Methoden werden durch weitere Richtlinien unter Berücksichtigung des aktuellen Stands von Wissenschaft und Technik sowie bewährter Praktiken festgelegt.
Für die Festlegung und Weiterentwicklung dieser Verfahren wurde ein „Ständiger Ausschuss für Futtermittel“ eingesetzt, der in einem Schnellverfahren die Zusammenarbeit zwischen Kommission und Mitgliedstaaten organisiert.
Die Kommission nimmt Vorschläge für Maßnahmen auf Basis der Ausschuss-Empfehlungen an oder legt bei Abweichungen dem Rat Vorschläge vor, der mit qualifizierter Mehrheit entscheidet.
Die Mitgliedstaaten setzen erforderliche Rechts- und Verwaltungsvorschriften innerhalb eines Jahres nach Veröffentlichung der Richtlinie um und informieren die Kommission unverzüglich über die Umsetzung.
Betroffene Produkte und Akteure
Betroffen sind alle Futtermittel, die in den Mitgliedstaaten erzeugt, gehandelt oder verwendet werden.
Die Richtlinie richtet sich primär an die zuständigen Behörden der Mitgliedstaaten, die für die amtliche Untersuchung von Futtermitteln verantwortlich sind.
Beteiligte Akteure sind ferner die Hersteller und Händler von Futtermitteln, da deren Produkte den einheitlichen amtlichen Prüfungen unterliegen.
Umsetzungszeitplan
Die Mitgliedstaaten mussten die notwendigen Rechts- und Verwaltungsvorschriften binnen eines Jahres nach Bekanntmachung der Richtlinie in Kraft setzen.
Die Einführung und der Fortschritt der gemeinschaftlichen Probenahmeverfahren und Analysemethoden erfolgt schrittweise im Rahmen weiterer spezifischer Richtlinien, deren Verfahren innerhalb von 18 Monaten nach Einsetzung des Ausschusses anzuwenden sind.
Die Festlegung einzelner Probenahme- und Analysemethoden erfolgt fortlaufend nach dem beschriebenen Ausschussverfahren.
Diese Richtlinie bildet somit die Grundlage für die Harmonisierung der amtlichen Kontrollen von Futtermitteln in der EU, fördert einheitliche Qualitätsstandards und unterstützt den Binnenmarkt durch rechtliche und technische Angleichung.
Die Richtlinie 70/373/EG findet Anwendung auf amtliche Untersuchungen von Futtermitteln in der Europäischen Wirtschaftsgemeinschaft. Sie regelt die Einführung gemeinschaftlicher Probenahmeverfahren und Analysemethoden, die von den Mitgliedstaaten bei der Überprüfung der Beschaffenheit und Zusammensetzung von Futtermitteln anzuwenden sind. Ziel ist es, Unterschiede zwischen den bislang angewendeten nationalen Verfahren zu harmonisieren, um einen einheitlichen Binnenmarkt zu gewährleisten. Die Richtlinie gilt für alle Futtermittel, die innerhalb der Gemeinschaft erzeugt, gehandelt oder verwendet werden, und betrifft insbesondere amtliche Kontrollen, welche die Einhaltung rechtlicher Vorschriften sicherstellen sollen. Die Verfahren und Methoden werden unter Berücksichtigung wissenschaftlicher Entwicklungen und bewährter Praktiken in weiteren Richtlinien festgelegt.
La directive 70/373/CEE vise à harmoniser au sein de la Communauté économique européenne les modes de prélèvement d’échantillons et les méthodes d’analyse pour le contrôle officiel des aliments des animaux. Elle souligne l'importance de garantir la qualité et la composition des aliments animaux afin de sécuriser la production agricole et d'assurer un fonctionnement optimal du marché commun. Pour ce faire, elle impose aux États membres de réaliser les contrôles selon des méthodes communautaires uniformes, établies par des directives basées sur des connaissances scientifiques et techniques. Un Comité permanent des aliments des animaux est institué pour proposer et adopter rapidement ces mesures techniques. La Commission européenne prend les décisions en fonction des avis du Comité, avec possibilité d’intervention du Conseil en cas de désaccord. Les États membres doivent transposer ces mesures dans leur droit national dans un délai d’un an après la notification de la directive, afin d’harmoniser les pratiques de contrôle dans l’ensemble de la Communauté. Cette directive constitue une étape fondamentale dans la normalisation du contrôle des aliments pour animaux, contribuant à la qualité et à la sécurité dans la chaîne alimentaire.
Objet
La directive 70/373/CEE du Conseil du 20 juillet 1970 vise à harmoniser au niveau communautaire les modes de prélèvement d’échantillons et les méthodes d’analyse officiels dans le cadre du contrôle des aliments pour animaux. Elle répond à la nécessité d’assurer la qualité et la conformité des aliments pour animaux utilisés dans la Communauté économique européenne (CEE) afin d’améliorer la productivité agricole et de garantir le bon fonctionnement du marché commun. La directive propose ainsi une base pour des contrôles uniformes en matière de qualité et de composition des aliments pour animaux à travers tous les États membres.
Obligations principales
Mise en place de contrôles officiels : Les États membres doivent assurer que les contrôles officiels destinés à vérifier la conformité des aliments pour animaux avec les exigences législatives, réglementaires et administratives s’appuient sur des modes de prélèvement d’échantillons et des méthodes d’analyse unifiés à l’échelle communautaire.
Élaboration commune des modes et méthodes : Ces modes de prélèvement et méthodes d’analyse sont fixés par directives adoptées selon une procédure précise impliquant un comité permanent des aliments pour animaux, garantissant la prise en compte des connaissances scientifiques et techniques disponibles ainsi que des pratiques existantes.
Procédure d’adoption rapide et concertée : La directive établit une procédure de coopération étroite entre la Commission et les États membres via un comité permanent, permettant une adaptation rapide et technique des méthodes selon les besoins.
Respect des délais : Les États membres doivent intégrer les modes de prélèvement et méthodes d’analyse communautaires dans leur droit national dans des délais fixés dans les directives spécifiques.
Notification : Après adoption de la directive, chaque État membre doit notifier à la Commission les mesures législatives, réglementaires et administratives prises pour s’y conformer.
Produits et acteurs concernés
Produits concernés : Tous les aliments destinés à l’alimentation des animaux dans les États membres de la Communauté économique européenne.
Acteurs impactés :
- Les autorités nationales responsables du contrôle officiel des aliments pour animaux dans chaque État membre ;
- Les laboratoires et services chargés du prélèvement d’échantillons et des analyses ;
- Les producteurs, distributeurs et utilisateurs d’aliments pour animaux soucieux de conformité au cadre règlementaire communautaire.
Calendrier de mise en œuvre
Délai d’adoption des modes et méthodes : Les directives fixant les méthodes et modes de prélèvement doivent prévoir des délais raisonnables pour leur introduction dans les législations nationales.
Entrée en vigueur nationale : Les États membres disposent d’un délai d’un an à compter de la notification de la présente directive (c’est-à-dire à partir du 20 juillet 1970) pour adopter les mesures nécessaires en droit national.
Durée de la procédure de comitologie : La procédure avec le Comité permanent des aliments des animaux est applicable pendant une période de dix-huit mois à partir de sa première saisine.
Cette directive constitue un pilier technique pour assurer la fiabilité et l’harmonisation des contrôles officiels visant à garantir la qualité des aliments pour animaux sur tout le territoire de la Communauté économique européenne.
La directive 70/373/CEE s’applique au contrôle officiel des aliments des animaux au sein de la Communauté économique européenne. Elle concerne la production, la commercialisation et l’utilisation des aliments pour animaux, qui sont essentiels pour la productivité agricole. Son objectif est d’harmoniser les modes de prélèvement d’échantillons et les méthodes d’analyse utilisés dans les contrôles officiels afin de garantir le respect des normes de qualité et de composition des aliments pour animaux. Ces règles s’adressent à toutes les autorités chargées du contrôle officiel dans les États membres, afin d’assurer une application uniforme des normes législatives, réglementaires et administratives relatives à ces produits dans toute la Communauté.
Direktiva 70/373/EGS uvaja enotne metode vzorčenja in analize za uradni nadzor kakovosti in sestave krme v državah članicah Evropske gospodarske skupnosti. Cilj je zagotoviti skladnost z zakonodajnimi zahtevami ter povečati kmetijsko produktivnost z uporabo kakovostne krme. Države članice morajo sprejeti potrebne ukrepe, da uradni nadzor temelji na skupnih tehničnih in znanstvenih metodah, določenih z nadaljnjimi direktivami, pri čemer se upošteva najnovejša znanost in tehnika. Uveden je postopek sodelovanja med državami članicami in Komisijo prek Stalnega odbora za krmo za sprejemanje in izboljševanje teh metod. Direktiva določa primere za sprejem zakonodaje o uskladitvi v enem letu od notifikacije ter postopke odločanja pri nesoglasjih. S tem se zagotavlja enoten pristop k nadzoru krme, kar vpliva na ureditev in delovanje skupnega trga Skupnosti.
Namen
Direktiva 70/373/EGS z dne 20. julija 1970 določa uvedbo enotnih metod vzorčenja in analiz v okviru Skupnosti za uradni nadzor krme. Glavni cilj direktive je zagotoviti, da se dejavnosti proizvodnje, trženja in uporabe krme izvajajo po standardiziranih in znanstveno preverjenih metodah, ki omogočajo usklajeno preverjanje kakovosti in sestave krme v vseh državah članicah. S tem se krepi zanesljivost uradnega nadzora, izboljšuje kakovost živalske hrane in s tem povečuje kmetijska produktivnost ter omogoča učinkovito delovanje skupnega trga.
Ključne obveznosti
- Države članice morajo sprejeti ustrezne ukrepe, s katerimi zagotovijo, da se uradni nadzor krme izvaja po metodah vzorčenja in analiz, predpisanih s strani Skupnosti in določenih v nadaljnjih direktivah.
- Metode vzorčenja in analize morajo biti enotne, znanstveno preverjene ter v skladu s sodobno tehnologijo, da omogočajo transparentno in dosledno preverjanje skladnosti z zakonodajnimi določbami vsake države.
- Za sprejemanje in posodabljanje teh metod je vzpostavljen poseben postopek, ki vključuje sodelovanje Stalnega odbora za krmo.
- Države članice morajo v enem letu po obvestilu Evropske komisije uskladiti svojo zakonodajo z direktivo.
- Sprejeti mora biti hiter in učinkovit postopek razvoja, izpopolnjevanja in utrjevanja metod vzorčenja in analiz, v sodelovanju med državami in Komisijo.
Vplivani izdelki in subjekti
- Direktivno področje zajema vse krmne izdelke, namenjene živalski proizvodnji v državah članicah.
- Na direktivo so zavezani nacionalni organi za uradni nadzor krme, ki morajo izvajati vzorčenje in analize skladno z določenimi metodami.
- Skladnost z metodami vpliva na proizvajalce, distributerje in uporabnike krme, saj zdravstvena in kakovostna skladnost neposredno vplivata na trg in varnost živalske proizvodnje.
Časovni okvir za izvedbo
- Direktiva je začela veljati z dnem njene objave 20. julija 1970.
- Države članice morajo v enem letu od njenega notifikacije sprejeti nacionalne predpise, ki uskladijo državno zakonodajo z določbami direktive.
- Postopek za sprejem in razvoj metod vzorčenja in analiz se izvaja preko Stalnega odbora za krmo, pri čemer so predvideni roki za posvetovanja in odločanje Komisije in Sveta.
- Člen 4 določa, da se določbe postopka za sprejem ukrepov uporabljajo osemnajst mesecev od prve predložitve zadeve odboru, kar omogoča stabilnost in čas za usklajevanje med državami.
Direktiva 70/373/EGS se nanaša na uradni nadzor kakovosti in sestave krme, ki se uporablja v živinoreji. Namenjena je zagotavljanju enotnih metod vzorčenja in analiz za preverjanje skladnosti krme z zakonskimi ali drugimi predpisi o njeni kakovosti in sestavi. Uveljavlja se v sektorju kmetijstva, kjer je kakovost krme ključna za živalsko proizvodnjo. Direktiva obsega proizvodnjo, trženje in uporabo krme ter določa, da morajo države članice uporabiti enotne, skupnostno določene metode za vzorčenje in analizo krme, da se omogoči učinkovit in usklajen nadzor po vsej Skupnosti in s tem vpliva na delovanje skupnega trga.
General Information
ISO 6498:2012 specifies guidelines for the preparation of test samples from laboratory samples of animal feeding stuffs, including pet foods.
The guidelines are overruled by special instructions and regulations for sample preparation demanded by specific analysis methods.
- Standard53 pagesEnglish languagee-Library read for1 day
ISO 6498:2012 specifies guidelines for the preparation of test samples from laboratory samples of animal feeding stuffs, including pet foods.
The guidelines are overruled by special instructions and regulations for sample preparation demanded by specific analysis methods.
- Standard53 pagesEnglish languagee-Library read for1 day
This European Standard specifies an Ion-Selective Electrode method (ISE) after hydrochloric acid treatment for the determination of fluoride from animal feeding stuffs. The content of fluoride (F-) corresponds to that of fluorine (F) specified in Commission Regulation (EU) 574/2011[3].
This European Standard is strictly based on several conventions such as those contained in the following example:
EXAMPLE 0,5 g test portion for extraction of fluoride from animal feeds by means of an acid treatment with 20 ml of 1 mol/l hydrochloric acid solution at ambient temperature (20 °C to 25 ºC) for 20 min. The pH is controlled and adjusted to 5,5 in the buffered test solution before determination of fluoride by ISE using standard addition technique.
The method was successfully tested in an interlaboratory study in concentrations between 100 mg/kg up to 500 mg/kg. If this method is followed strictly, then theoretically all concentrations from 40 mg/kg up to 4 000 mg/kg can be analysed within the linear calibration function.
Only for concentrations lower than 40 mg/kg is the use of an interpolation technique required instead of standard addition Annex C.
The quantification limit for fluoride using the conventions of the method including the standard addition technique is 40 mg/kg or lower than 2,5 mg/kg when using interpolation Annex C.
- Standard18 pagesEnglish languagee-Library read for1 day
This European Standard describes a procedure for the determination of inorganic arsenic in animal feeding stuffs of marine origin by Solid Phase Extraction (SPE) and Hydride Generation Atomic Absorption Spectrometry (HG-AAS). The method has been successfully tested in a collaborative trial with a working range from 0,19 mg/kg to 2,7 mg/kg (HORRATvalues < 2). The LOQ of the method is usually approximately 0,1 mg/kg or lower.
- Standard18 pagesEnglish languagee-Library read for1 day
This European Standard specifies a method for the determination of mercury in animal feeding stuffs by Cold-Vapour Atomic Absorption Spectrometry (CVAAS) after microwave pressure digestion. The limit of quantification in the test solution should be 0,25 µg/l or lower. Using a test portion of 0,5 g and a volume of the test solution of 25 ml a limit of quantification of 0,0125 mg/kg or lower should be obtained.
- Standard17 pagesEnglish languagee-Library read for1 day
This European Standard is applicable to the quantitative analysis of (bound and free) hydrocyanic acid (HCN) in feed materials of plant origin and compound feed by High Performance Liquid Chromatography (HPLC).
The method is validated from 10 mg HCN/kg to 350 mg HCN/kg. When the method is used outside this range it should be validated at least within the laboratory. A limit of quantification of 2 mg HCN/kg should normally be obtained.
- Standard16 pagesEnglish languagee-Library read for1 day
This European Standard specifies a method for the determination of decoquinate. This high-performance liquid chromatographic (HPLC) method with a fluorescence detection is applicable to the quantification of decoquinate content in complete and complementary compound feeds, medicated feeds, semi-liquid feeds, premixtures and feed additives.
The method was fully validated from LOQ to 60 000 mg/kg on different matrices during an international collaborative study [11], especially on complete compound feeds for poultry, at trace contamination level of 3 mg/kg and at European authorized level of 20 mg/kg to 40 mg/kg [12].
The limit of detection is between 0,1 mg/kg and 0,3 mg/kg and the limit of quantification is around 0,5 mg/kg. These limits were validated during the collaborative study [11], from results on the blank feed. Lower limits of detection or quantification could be reached but a single laboratory validation is then requested.
- Standard24 pagesEnglish languagee-Library read for1 day
This European Standard specifies a method for the determination of mercury in animal feeding stuffs by Cold-Vapour Atomic Absorption Spectrometry (CVAAS) after microwave pressure digestion. The limit of quantification in the test solution should be 0,25 µg/l or lower. Using a test portion of 0,5 g and a volume of the test solution of 25 ml a limit of quantification of 0,0125 mg/kg or lower should be obtained.
- Standard17 pagesEnglish languagee-Library read for1 day
This European Standard describes a procedure for the determination of inorganic arsenic in animal feeding stuffs of marine origin by Solid Phase Extraction (SPE) and Hydride Generation Atomic Absorption Spectrometry (HG-AAS). The method has been successfully tested in a collaborative trial with a working range from 0,19 mg/kg to 2,7 mg/kg (HORRATvalues < 2). The LOQ of the method is usually approximately 0,1 mg/kg or lower.
- Standard18 pagesEnglish languagee-Library read for1 day
This European Standard specifies an Ion-Selective Electrode method (ISE) after hydrochloric acid treatment for the determination of fluoride from animal feeding stuffs. The content of fluoride (F-) corresponds to that of fluorine (F) specified in Commission Regulation (EU) 574/2011[3].
This European Standard is strictly based on several conventions such as those contained in the following example:
EXAMPLE 0,5 g test portion for extraction of fluoride from animal feeds by means of an acid treatment with 20 ml of 1 mol/l hydrochloric acid solution at ambient temperature (20 °C to 25 ºC) for 20 min. The pH is controlled and adjusted to 5,5 in the buffered test solution before determination of fluoride by ISE using standard addition technique.
The method was successfully tested in an interlaboratory study in concentrations between 100 mg/kg up to 500 mg/kg. If this method is followed strictly, then theoretically all concentrations from 40 mg/kg up to 4 000 mg/kg can be analysed within the linear calibration function.
Only for concentrations lower than 40 mg/kg is the use of an interpolation technique required instead of standard addition Annex C.
The quantification limit for fluoride using the conventions of the method including the standard addition technique is 40 mg/kg or lower than 2,5 mg/kg when using interpolation Annex C.
- Standard18 pagesEnglish languagee-Library read for1 day
This European Standard specifies a high-performance liquid chromatographic (HPLC) method for the determination of the monensin, narasin and salinomycin contents of animal feeding stuffs, supplements (dry and liquid) and mineral premixtures. The method is not applicable to drug premixes (pharmaceutical products). Lasalocid and semduramicin cannot be determined by this method. The limit of quantitation is approximately 1 mg/kg, 2 mg/kg and 2 mg/kg for monensin, salinomycin and narasin, respectively. A lower limit of quantitation can be achievable but this is to be validated by the user.
- Corrigendum4 pagesFrench languagee-Library read for1 day
This European Standard specifies a method for the determination of decoquinate. This high-performance liquid chromatographic (HPLC) method with a fluorescence detection is applicable to the quantification of decoquinate content in complete and complementary compound feeds, medicated feeds, semi-liquid feeds, premixtures and feed additives.
The method was fully validated from LOQ to 60 000 mg/kg on different matrices during an international collaborative study [11], especially on complete compound feeds for poultry, at trace contamination level of 3 mg/kg and at European authorized level of 20 mg/kg to 40 mg/kg [12].
The limit of detection is between 0,1 mg/kg and 0,3 mg/kg and the limit of quantification is around 0,5 mg/kg. These limits were validated during the collaborative study [11], from results on the blank feed. Lower limits of detection or quantification could be reached but a single laboratory validation is then requested.
- Standard24 pagesEnglish languagee-Library read for1 day
This European Standard is applicable to the quantitative analysis of (bound and free) hydrocyanic acid (HCN) in feed materials of plant origin and compound feed by High Performance Liquid Chromatography (HPLC).
The method is validated from 10 mg HCN/kg to 350 mg HCN/kg. When the method is used outside this range it should be validated at least within the laboratory. A limit of quantification of 2 mg HCN/kg should normally be obtained.
- Standard16 pagesEnglish languagee-Library read for1 day
This European Standard specifies a method for the determination of total arsenic in animal feeding stuffs by hydride generation atomic absorption spectrometry (HGAAS) after microwave pressure digestion. The limit of quantification is 0,5 µg/l of the test solution. Using a test portion of 0,5 g, a volume of the test solution of 25 ml and an aliquot of 5 ml for pre-reduction the limit of quantification is 0,125 mg/kg in the feed material.
NOTE For feed materials containing organic arsenic species from compounds of marine origin (i.e. arsenobetaine and tetramethylarsine oxide) a higher digestion temperature of the microwave system up to 300 °C may be necessary in order to enable the hydridisation of these arsenic compounds and in order to determine all different kinds of arsenic species in the corresponding feeding stuffs. Alternatively, the digestion procedure of Annex D can be used if the microwave system does not reach higher temperatures up to 300 °C to ensure complete mineralization for HGAAS determination.
- Standard16 pagesEnglish languagee-Library read for1 day
This European Standard specifies a method for the determination of selenium in animal feeding stuffs by hydride generation atomic absorption spectrometry (HGAAS) after microwave pressure digestion.
The method was successfully tested by an inter-laboratory study of CEN/TC 327/WG 4 in the range of 0,25 mg/kg to 74 mg/kg.
The limit of quantification is 0,5 µg/l of the test solution which corresponds to the calibration standard 2. Using a test portion of 0,5 g and a volume of the test solution of 25 ml after pressure digestion the limit of quantification is calculated as 0,125 mg/kg in the feed material.
NOTE A lower limit of quantification could be achieved – each laboratory has to prove it.
- Standard16 pagesEnglish languagee-Library read for1 day
This European standard specifies a high-performance liquid chromatographic (HPLC) method for the determination of the semduramicin content at authorized level in animal feeding stuffs [2], using mass spectrometry detection or post-column derivatization and (UV)-VIS detection (hereinafter UV detection). This method is applicable to poultry feed. The limit of quantitation is 1,0 mg/kg when mass spectrometry is used for detection and 3,0 mg/kg when the detection is performed by UV with post-column derivatization. Lower limits of quantitation are achievable but this is to be validated by the user.
The method allows the discrimination of semduramicin from monensin, salinomycin, narasin, maduramicin and lasalocid.
- Standard25 pagesEnglish languagee-Library read for1 day
2010-03-19 EMA: Corrigendum on the French version only.
- Corrigendum4 pagesFrench languagee-Library read for1 day
This European standard specifies a high-performance liquid chromatographic (HPLC) method for the determination of the semduramicin content at authorized level in animal feeding stuffs [2], using mass spectrometry detection or post-column derivatization and (UV)-VIS detection (hereinafter UV detection). This method is applicable to poultry feed. The limit of quantitation is 1,0 mg/kg when mass spectrometry is used for detection and 3,0 mg/kg when the detection is performed by UV with post-column derivatization. Lower limits of quantitation are achievable but this is to be validated by the user.
The method allows the discrimination of semduramicin from monensin, salinomycin, narasin, maduramicin and lasalocid.
- Standard25 pagesEnglish languagee-Library read for1 day
This European Standard specifies a method for the determination of total arsenic in animal feeding stuffs by hydride generation atomic absorption spectrometry (HGAAS) after microwave pressure digestion. The limit of quantification is 0,5 µg/l of the test solution. Using a test portion of 0,5 g, a volume of the test solution of 25 ml and an aliquot of 5 ml for pre-reduction the limit of quantification is 0,125 mg/kg in the feed material.
NOTE For feed materials containing organic arsenic species from compounds of marine origin (i.e. arsenobetaine and tetramethylarsine oxide) a higher digestion temperature of the microwave system up to 300 °C may be necessary in order to enable the hydridisation of these arsenic compounds and in order to determine all different kinds of arsenic species in the corresponding feeding stuffs. Alternatively, the digestion procedure of Annex D can be used if the microwave system does not reach higher temperatures up to 300 °C to ensure complete mineralization for HGAAS determination.
- Standard16 pagesEnglish languagee-Library read for1 day
This European Standard specifies a method for the determination of selenium in animal feeding stuffs by hydride generation atomic absorption spectrometry (HGAAS) after microwave pressure digestion.
The method was successfully tested by an inter-laboratory study of CEN/TC 327/WG 4 in the range of 0,25 mg/kg to 74 mg/kg.
The limit of quantification is 0,5 µg/l of the test solution which corresponds to the calibration standard 2. Using a test portion of 0,5 g and a volume of the test solution of 25 ml after pressure digestion the limit of quantification is calculated as 0,125 mg/kg in the feed material.
NOTE A lower limit of quantification could be achieved – each laboratory has to prove it.
- Standard16 pagesEnglish languagee-Library read for1 day
This European Standard is applicable to the determination of Fumonisin B1 & B2 (FB1 & FB2) in compound animal feed at levels starting from 3 mg/kg up to 16 mg/kg.
- Standard23 pagesEnglish languagee-Library read for1 day
This European Standard specifies a method for the determination of Ochratoxin A (OTA) in cereal based animal feed using immunoaffinity for clean-up followed by liquid-chromatography with fluorescence detection.
NOTE The validated mass fraction range was 39 µg/kg to 338 µg/kg OTA.
- Standard18 pagesEnglish languagee-Library read for1 day
This European Standard is applicable to the determination of Fumonisin B1 & B2 (FB1 & FB2) in compound animal feed at levels starting from 3 mg/kg up to 16 mg/kg.
- Standard23 pagesEnglish languagee-Library read for1 day
This European Standard specifies a method for the determination of Ochratoxin A (OTA) in cereal based animal feed using immunoaffinity for clean-up followed by liquid-chromatography with fluorescence detection.
NOTE The validated mass fraction range was 39 µg/kg to 338 µg/kg OTA.
- Standard18 pagesEnglish languagee-Library read for1 day
This European Standard defines general rules for the enumeration of probiotic bifidobacteria in feed samples (additives, premixtures and feeding stuffs) that contain bifidobacteria as a single bacterial component or in a mixture with other microorganisms. This standard is not applicable for mineral feeds which are defined as complementary feeding stuffs composed mainly of minerals and containing at least 40% crude ash (Council Directive 79/373/EEC) [3].
There are different categories of feed samples:
a) Additives containing about 1010 colony forming units (CFU)/g
b) Premixtures containing about 108 CFU/g
c) Feeds, meal or pellets, which contain about 106 CFU/g and include complete feeding stuffs, and milk replacers.
The detection limit is as defined in ISO 7218.
- Standard16 pagesEnglish languagee-Library read for1 day
This European Standard specifies a method for the determination of additive use of nicarbazin in animal feeding stuffs and premixtures (maximum concentration 2,5% nicarbazin) using high performance liquid chromatography. Nicarbazin is a 1:1 equimolar mixture of 4,4’-dinitrocarbanilide (DNC) and 4,6-dimethyl-2-pyriminol (HDP). Nicarbazin is generally determined by using DNC as the target compound. In this method the DNC moiety of nicarbazin is detected.
The limit of quantitation is 20 mg/kg. The limit of detection is 0,5 mg/kg
NOTE A lower limit of quantitation may be achievable but should be validated by the user.
- Standard10 pagesEnglish languagee-Library read for1 day
This European Standard specifies a high performance liquid chromatography (HPLC) method for the
determination of the content of maduramicin in feeding stuffs and premixtures.
The usual concentration of maduramicin in feedstuffs is 5 mg/kg, in premixtures 500 mg/kg. The limit of
quantification is 2 mg/kg. The limit of detection is 0,5 mg/kg.
NOTE A lower limit of quantification may be achievable but shall be validated by the user.
- Standard13 pagesEnglish languagee-Library read for1 day
This European Standard defines general rules for the enumeration of probiotic bifidobacteria in feed samples (additives, premixtures and feeding stuffs) that contain bifidobacteria as a single bacterial component or in a mixture with other microorganisms. This standard is not applicable for mineral feeds which are defined as complementary feeding stuffs composed mainly of minerals and containing at least 40% crude ash (Council Directive 79/373/EEC) [3].
There are different categories of feed samples:
a) Additives containing about 1010 colony forming units (CFU)/g
b) Premixtures containing about 108 CFU/g
c) Feeds, meal or pellets, which contain about 106 CFU/g and include complete feeding stuffs, and milk replacers.
The detection limit is as defined in ISO 7218.
- Standard16 pagesEnglish languagee-Library read for1 day
This Standard is applicable to the determination of deoxynivalenol (DON) in animal compound feed at concentrations of 150 μg/kg up to at least 4 000 μg/kg.
- Standard15 pagesEnglish languagee-Library read for1 day
This International Standard is applicable to the determination of zearalenone in animal feed at concentrations from 30 µg/kg to 3 000 µg/kg.
- Standard15 pagesEnglish languagee-Library read for1 day
This International Standard is applicable to the determination of zearalenone in animal feed at concentrations from 30 µg/kg to 3 000 µg/kg.
- Standard15 pagesEnglish languagee-Library read for1 day
This Standard is applicable to the determination of deoxynivalenol (DON) in animal compound feed at concentrations of 150 μg/kg up to at least 4 000 μg/kg.
- Standard15 pagesEnglish languagee-Library read for1 day
This European Standard specifies a method for the determination of additive use of nicarbazin in animal feeding stuffs and premixtures (maximum concentration 2,5% nicarbazin) using high performance liquid chromatography. Nicarbazin is a 1:1 equimolar mixture of 4,4’-dinitrocarbanilide (DNC) and 4,6-dimethyl-2-pyriminol (HDP). Nicarbazin is generally determined by using DNC as the target compound. In this method the DNC moiety of nicarbazin is detected.
The limit of quantitation is 20 mg/kg. The limit of detection is 0,5 mg/kg
NOTE A lower limit of quantitation may be achievable but should be validated by the user.
- Standard10 pagesEnglish languagee-Library read for1 day
This European Standard specifies a high performance liquid chromatography (HPLC) method for the
determination of the content of maduramicin in feeding stuffs and premixtures.
The usual concentration of maduramicin in feedstuffs is 5 mg/kg, in premixtures 500 mg/kg. The limit of
quantification is 2 mg/kg. The limit of detection is 0,5 mg/kg.
NOTE A lower limit of quantification may be achievable but shall be validated by the user.
- Standard13 pagesEnglish languagee-Library read for1 day
ISO 14183:2005 specifies a high-performance liquid chromatographic (HPLC) method for the determination of the monensin, narasin and salinomycin contents of animal feeding stuffs, supplements (dry and liquid) and mineral premixtures. The method is not applicable to drug premixes (pharmaceutical products). Lasalocid and semduramicin cannot be determined by this method.
The limit of quantitation is approximately 1 mg/kg, 2 mg/kg and 2 mg/kg for monensin, salinomycin and narasin, respectively. A lower limit of quantitation can be achievable but this is to be validated by the user.
- Standard28 pagesEnglish languagee-Library read for1 day
ISO 16634-1:2008 specifies a method for the determination of the total nitrogen content and the calculation of crude protein content of oilseeds and animal feeding stuffs.
This method, like the Kjeldahl method, does not distinguish between protein nitrogen and non-protein nitrogen. For the calculation of protein content, various conversion factors are used.
This method is not applicable to milk and milk products.
- Standard38 pagesEnglish languagee-Library read for1 day
This Technical Specification defines a polymerase chain reaction (PCR) methodology for the identification of S. cerevisiae probiotic yeast strains. Additionally, a method for the extraction of high quality DNA from yeast is suggested.
- Technical specification12 pagesEnglish languagee-Library read for1 day
ISO 14183:2005 specifies a high-performance liquid chromatographic (HPLC) method for the determination of the monensin, narasin and salinomycin contents of animal feeding stuffs, supplements (dry and liquid) and mineral premixtures. The method is not applicable to drug premixes (pharmaceutical products). Lasalocid and semduramicin cannot be determined by this method.
The limit of quantitation is approximately 1 mg/kg, 2 mg/kg and 2 mg/kg for monensin, salinomycin and narasin, respectively. A lower limit of quantitation can be achievable but this is to be validated by the user.
- Standard28 pagesEnglish languagee-Library read for1 day
This Technical Specification defines a polymerase chain reaction (PCR) methodology for the identification of S. cerevisiae probiotic yeast strains. Additionally, a method for the extraction of high quality DNA from yeast is suggested.
- Technical specification12 pagesEnglish languagee-Library read for1 day
ISO 16634-1:2008 specifies a method for the determination of the total nitrogen content and the calculation of crude protein content of oilseeds and animal feeding stuffs.
This method, like the Kjeldahl method, does not distinguish between protein nitrogen and non-protein nitrogen. For the calculation of protein content, various conversion factors are used.
This method is not applicable to milk and milk products.
- Standard38 pagesEnglish languagee-Library read for1 day
This Technical Specification describes the quantitative determination of specific sugars (glucose, fructose, galactose, sucrose, maltose, and lactose) in dry animal feeding stuffs at the g/kg level by a sophisticated high performance anion exchange chromatography in combination with pulsed amperometric detection (HPAEC-PAD).
- Technical specification20 pagesEnglish languagee-Library read for1 day
This Technical Specification describes the quantitative determination of specific sugars (glucose, fructose, galactose, sucrose, maltose, and lactose) in dry animal feeding stuffs at the g/kg level by a sophisticated high performance anion exchange chromatography in combination with pulsed amperometric detection (HPAEC-PAD).
- Technical specification20 pagesEnglish languagee-Library read for1 day
ISO 17375:2006 specifies a method for the determination of aflatoxin B1 in animal feeding stuffs using high-performance liquid chromatography with post-column derivatization.
It is applicable to animal feeding stuffs with a fat content of up to 50 %.
The limit of quantification of this method has been demonstrated to be better than 0,5 g/kg for aflatoxin B1 for a signal-to-noise ratio of 6.
- Standard17 pagesEnglish languagee-Library read for1 day
ISO 17375:2006 specifies a method for the determination of aflatoxin B1 in animal feeding stuffs using high-performance liquid chromatography with post-column derivatization.
It is applicable to animal feeding stuffs with a fat content of up to 50 %.
The limit of quantification of this method has been demonstrated to be better than 0,5 g/kg for aflatoxin B1 for a signal-to-noise ratio of 6.
- Standard17 pagesEnglish languagee-Library read for1 day
ISO 15914:2004 specifies a method for the enzymatic determination of the total starch content of animal feeding stuffs and raw materials for animal feeding stuffs.
The method is also applicable to the determination of starch in starch.
It is important that in the sample matrix no components are present which contribute to the measured extinction at 340 nm.
The analytical range of the method is 40 g/kg to 1 000 g/kg starch. The standard procedure is applicable to the range 200 g/kg to 1000 g/kg. For the lower range, 40 g/kg to 200 g/kg, another dilution procedure for the standard glucose solution and samples can be used.
- Standard14 pagesEnglish languagee-Library read for1 day
ISO 6497:2002 specifies methods of sampling animal feeding stuffs, including fish feed, for quality control for commercial, technical and legal purposes.
It is not applicable to pet foods. Nor are the methods intended for sampling for the purpose of microbiological examination. Conditions of, and requirements for, sampling are specified separately for feeding stuffs of different physical natures.
For certain categories of animal feeding stuff, specific methods of sampling are specified in other International Standards. A list of these can be found in the bibliography. When sampling the products specified, it is these methods which shall be used.
Methods of sampling for the determination of substances likely to be non-uniformly distributed are described in an annex.
- Standard21 pagesEnglish languagee-Library read for1 day
ISO 15914:2004 specifies a method for the enzymatic determination of the total starch content of animal feeding stuffs and raw materials for animal feeding stuffs.
The method is also applicable to the determination of starch in starch.
It is important that in the sample matrix no components are present which contribute to the measured extinction at 340 nm.
The analytical range of the method is 40 g/kg to 1 000 g/kg starch. The standard procedure is applicable to the range 200 g/kg to 1000 g/kg. For the lower range, 40 g/kg to 200 g/kg, another dilution procedure for the standard glucose solution and samples can be used.
- Standard14 pagesEnglish languagee-Library read for1 day
ISO 6497:2002 specifies methods of sampling animal feeding stuffs, including fish feed, for quality control for commercial, technical and legal purposes.
It is not applicable to pet foods. Nor are the methods intended for sampling for the purpose of microbiological examination. Conditions of, and requirements for, sampling are specified separately for feeding stuffs of different physical natures.
For certain categories of animal feeding stuff, specific methods of sampling are specified in other International Standards. A list of these can be found in the bibliography. When sampling the products specified, it is these methods which shall be used.
Methods of sampling for the determination of substances likely to be non-uniformly distributed are described in an annex.
- Standard21 pagesEnglish languagee-Library read for1 day
This International Standard specifies a method for the determination of the trypsin inhibitor activity (TIA) of soya
products.
This trypsin inhibitor activity is indicative of the degree of toasting of these products.
The detection limit of the method is 0,5 mg/g.
- Standard13 pagesEnglish languagee-Library read for1 day
This International Standard specifies a method for the determination of the trypsin inhibitor activity (TIA) of soya
products.
This trypsin inhibitor activity is indicative of the degree of toasting of these products.
The detection limit of the method is 0,5 mg/g.
- Standard13 pagesEnglish languagee-Library read for1 day
Frequently Asked Questions
An EU Directive is a legislative act of the European Union that sets out goals that all EU member states must achieve. However, it is up to each member state to devise their own laws on how to reach these goals through national transposition. Directives are used to harmonize laws across the EU, particularly for the functioning of the single market.
Directive 70/373/EC covers "Council Directive 70/373/EEC of 20 July 1970 on the introduction of Community methods of sampling and analysis for the official control of feeding-stuffs". There are 70 standards associated with this directive.
Harmonized standards under 70/373/EC are European standards (ENs) developed by CEN, CENELEC, or ETSI in response to a mandate from the European Commission. When these standards are cited in the Official Journal of the European Union, products manufactured in conformity with them benefit from a presumption of conformity with the essential requirements of 70/373/EC, facilitating CE marking and free movement within the European Economic Area.