ISO 13110:2025

International
Standard
ISO 13110
Second edition
Cigarettes — Determination of
2025-08
menthol in total particulate matter
from mainstream cigarette smoke
with a smoking regime according to
ISO 3308 (standard smoking regime)
— Gas chromatographic method
Cigarettes — Dosage du menthol dans la matière particulaire
totale du courant principal de la fumée de cigarette avec un
régime de fumage selon l'ISO 3308 (régime de fumage normalisé)
— Méthode par chromatographie en phase gazeuse
Reference number
© ISO 2025
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Published in Switzerland
ii
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 1
5 Reagents . 1
6 Apparatus . 2
7 Procedure . 3
7.1 Sampling .3
7.2 Test portion smoking and TPM extraction .3
7.3 Setting up the apparatus .3
7.4 Calibration of the gas chromatograph .4
7.5 Determination .4
8 Expression of results . 4
9 Repeatability and reproducibility . 4
9.1 Results of the 2010 ISO/TC 126 collaborative study .4
9.2 Results from the 2019 CORESTA collaborative study .5
10 Test report . 5
Annex A (normative) Smoking procedure for the determination of menthol in total particulate
matter from cigarette mainstream smoke . 6
Annex B (normative) Precautions to be taken for the sampling . 7
Annex C (informative) Example chromatogram . 8
Bibliography . 9

iii
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out through
ISO technical committees. Each member body interested in a subject for which a technical committee
has been established has the right to be represented on that committee. International organizations,
governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely
with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are described
in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the different types
of ISO documents should be noted. This document was drafted in accordance with the editorial rules of the
ISO/IEC Directives, Part 2 (see www.iso.org/directives).
ISO draws attention to the possibility that the implementation of this document may involve the use of (a)
patent(s). ISO takes no position concerning the evidence, validity or applicability of any claimed patent
rights in respect thereof. As of the date of publication of this document, ISO had not received notice of (a)
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related to conformity assessment, as well as information about ISO's adherence to the World Trade
Organization (WTO) principles in the Technical Barriers to Trade (TBT), see www.iso.org/iso/foreword.html.
This document was prepared by Technical Committee ISO/TC 126, Tobacco and tobacco products.
This second edition cancels and replaces the first edition (ISO 13110:2012), which has been technically
revised.
The main changes are as follows:
— references have been included in the bibliography section for the studies in 9.1 and 9.2;
— the term “condensate” has been replaced with “total particulate matter of mainstream cigarette smoke”;
— Clause 8 has been revised to be subclause 9.1 and to include the statistical analysis methods used;
— subclause 9.2 has been added to include the r and R estimates from the CORESTA 2020 collaborative study.
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www.iso.org/members.html.

iv
Introduction
Technical Committee ISO/TC 126 coordinated a collaborative study in 2010 for the determination of menthol
in mainstream cigarette smoke under ISO 4387.
The Cooperation Centre for Scientific Research Relative to Tobacco (CORESTA) Routine Analytical
Chemistry (RAC) Sub-Group conducted a collaborative study in 2019 that used the gas chromatographic
method identified in this document for the determination of menthol in mainstream cigarette smoke under
[1]
ISO 3308 and ISO 4387. A CORESTA technical report was published in 2020 for this collaborative study
[2]
involving 18 laboratories and four samples.
No machine smoking regimen can represent all human smoking behaviour:
— it is recommended that cigarettes also be tested under conditions of a different intensity of machine
smoking than those specified in this document;
— machine smoking testing is useful to characterize cigarette emissions for design and regulatory
purposes, but communication of machine measurements to smokers can result in misunderstandings
about differences in exposure and risk across brands;
— smoke emission data from machine measurements may be used as inputs for product hazard assessment,
but they are not intended to be nor are they valid as measures of human exposure or risks. Communicating
differences between products in machine measurements as differences in exposure or risk is a misuse of
testing using ISO standards.
v
International Standard ISO 13110:2025(en)
Cigarettes — Determination of menthol in total particulate
matter from mainstream cigarette smoke with a smoking
regime according to ISO 3308 (standard smoking regime) —
Gas chromatographic method
WARNING — The use of this document can involve hazardous materials, operations, and equipment.
This document does not purport to address all the safety problems associated with its use. It is the
responsibility of the user of this document to establish appropriate safety and health practices and
determine the applicability of any other restrictions prior to use.
1 Scope
This document specifies a method for the gas chromatographic determination of menthol in the total
particulate matter (TPM) of mentholated cigarette mainstream smoke with a smoking regime according
to ISO 3308 (standard smoking regime). The smoking of cigarettes and the collection of TPM from the
mainstream smoke are carried out in accordance with ISO 4387 with some exceptions (see Annex A).
Encapsulated menthol in specific products can lead to specific handling not described in this document.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content constitutes
requirements of this document. For dated references, only the edition cited applies. For undated references,
the latest edition of the referenced document (including any amendments) applies.
ISO 4387, Cigarettes — Determination of total and nicotine-free dry particulate matter using a routine
analytical smoking machine
ISO 8243, Cigarettes — Sampling
3 Terms and definitions
No terms and definitions are listed in this document.
ISO and IEC maintain terminology databases for use in standardization at the following addresses:
— ISO Online browsing platform: available at https:// www .iso .org/ obp
— IEC Electropedia: available at https:// www .electropedia .org/
4 Principle
The TPM from the mainstream smoke is dissolved in a solvent containing an internal standard. The menthol
content of an aliquot of the solution is determined by gas chromatography, and the menthol content of the
TPM of the mainstream smoke is calculated and expressed as milligram per cigarette.
5 Reagents
Use only reagents of recognized analytical grade.

5.1 Carrier gas, helium (
...