EN 15688:2008

SLOVENSKI STANDARD
01-oktober-2008
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Fertilizers - Determination of urease inhibitor N-(n-butyl)thiophosphoric triamide (NBPT)
using high-performance liquid chromatography (HPLC)
Düngemittel - Bestimmung von Urease-Hemmstoff N-(n-Butyl)-thiophosphortriamid
(NBPT) mit Hochleistungs-Flüssigchromatographie (HPLC)
Engrais - Détermination de l'inhibiteur d'uréase N-(n-butyl) thiophosphorique triamide
(NBPT) par chromatographie liquide haute performance (CLHP)
Ta slovenski standard je istoveten z: EN 15688:2008
ICS:
65.080 Gnojila Fertilizers
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.

EUROPEAN STANDARD
EN 15688
NORME EUROPÉENNE
EUROPÄISCHE NORM
August 2008
ICS 65.080
English Version
Fertilizers - Determination of urease inhibitor N-(n-
butyl)thiophosphoric triamide (NBPT) using high-performance
liquid chromatography (HPLC)
Engrais - Détermination de l'inhibiteur d'uréase N-(n-butyl) Düngemittel - Bestimmung von Urease-Hemmstoff N-(n-
triamine thiophosphorique (NBPT) par chromatographie Butyl)-thiophosphortriamid (NBPT) mit Hochleistungs-
liquide haute performance (HPLC) Flüssigchromatographie (HPLC)
This European Standard was approved by CEN on 4 July 2008.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European
Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national
standards may be obtained on application to the CEN Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation
under the responsibility of a CEN member into its own language and notified to the CEN Management Centre has the same status as the
official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland,
France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,
Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION
EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre: rue de Stassart, 36  B-1050 Brussels
© 2008 CEN All rights of exploitation in any form and by any means reserved Ref. No. EN 15688:2008: E
worldwide for CEN national Members.

Contents Page
Foreword.3
1 Scope .4
2 Normative references .4
3 Terms and definitions .4
4 Principle.4
5 Reagents.4
6 Apparatus .5
7 Sampling and sample preparation.6
8 Procedure .6
9 Calculation and expression of the result .7
10 Precision.7
11 Test report .8
Annex A (informative) Results of the inter-laboratory test .9
Bibliography .10

Foreword
This document (EN 15688:2008) has been prepared by Technical Committee CEN/TC 260 “Fertilizers and
liming materials”, the secretariat of which is held by DIN.
This European Standard shall be given the status of a national standard, either by publication of an identical
text or by endorsement, at the latest by February 2009, and conflicting national standards shall be withdrawn
at the latest by February 2009.
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. CEN [and/or CENELEC] shall not be held responsible for identifying any or all such patent rights.
This document has been prepared under a mandate given to CEN by the European Commission and the
European Free Trade Association.
According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following
countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Cyprus, Czech
Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia,
Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain,
Sweden, Switzerland and the United Kingdom.
1 Scope
This document specifies a method for the quantitative determination of the urease inhibitor N-(n-
butyl)thiophosphoric triamide (NBPT) content in water-soluble matrices, i.e. urea based fertilizers using high
performance liquid chromatography (HPLC).
NOTE Pure NBPT reference material is required as a standard for the quantitative calibration.
2 Normative references
The following referenced documents are indispensable for the application of this document. For dated
references, only the edition cited applies. For undated references, the latest edition of the referenced
document (including any amendments) applies.
EN 1482-2, Fertilizers and liming materials — Sampling and sample preparation — Part 2: Sample
preparation
EN 12944-1:1999, Fertilizers and liming materials and soil improvers — Vocabulary — Part 1: General terms
EN 12944-2:1999, Fertilizers and liming materials and soil improvers — Vocabulary — Part 2: Terms relating
to fertilizers
EN ISO 3696, Water for analytical laboratory use — Specification and test methods (ISO 3696:1987)
3 Terms and definitions
For the purposes of this document, the terms and definitions given in EN 12944-1:1999 and EN 12944-2:1999
apply.
4 Principle
This analytical method is based on the principles of liquid chromatography, with absorption in the ultraviolet
region for detection of the separated compounds.
5 Reagents
5.1 General
Use only reagents of recognized analytical grade and distilled or demineralized water (grade 3 according to
EN ISO 3696).
5.2 Reagents for liquid chromatography
5.2.1 Acetonitrile, HPLC-grade or spectroscopic grade.
5.2.2 Water, Milli-Q purified or equiva
...