EN 14166:2009

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Lebensmittel - Mikrobiologische Bestimmung von Vitamin B6Produits alimentaires - Détermination de la vitamine B6 par essai microbiologiqueFoodstuffs - Determination of vitamin B6 by microbiological assay07.100.30Mikrobiologija živilFood microbiologyICS:Ta slovenski standard je istoveten z:EN 14166:2009SIST EN 14166:2009en,fr,de01-december-2009SIST EN 14166:2009SLOVENSKI
STANDARDSIST ENV 14166:20021DGRPHãþD

EUROPEAN STANDARDNORME EUROPÉENNEEUROPÄISCHE NORMEN 14166May 2009ICS 07.100.30Supersedes ENV 14166:2001
English VersionFoodstuffs - Determination of vitamin B6 by microbiologicalassayProduits alimentaires - Détermination de la vitamine B6 paressai microbiologiqueLebensmittel - Mikrobiologische Bestimmung von VitaminB6This European Standard was approved by CEN on 23 April 2009.CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this EuropeanStandard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such nationalstandards may be obtained on application to the CEN Management Centre or to any CEN member.This European Standard exists in three official versions (English, French, German). A version in any other language made by translationunder the responsibility of a CEN member into its own language and notified to the CEN Management Centre has the same status as theofficial versions.CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland,France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.EUROPEAN COMMITTEE FOR STANDARDIZATIONCOMITÉ EUROPÉEN DE NORMALISATIONEUROPÄISCHES KOMITEE FÜR NORMUNGManagement Centre:
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B-1000 Brussels© 2009 CENAll rights of exploitation in any form and by any means reservedworldwide for CEN national Members.Ref. No. EN 14166:2009: ESIST EN 14166:2009

Vitamin B6 calibration lines obtained by MBA using the test organism Saccharomyces uvarum . 12Annex B (informative)
Precision data . 13Bibliography . 14 SIST EN 14166:2009

or suitable alternative) Dissolve the agar in glass distilled water according to the manufacturer’s instructions. Heat to boil. Dispense 5 ml aliquots into glass bottles, cap and autoclave at 121 °C for 15 min. Cool at an angle for slopes to form. Store in a refrigerator for up to three months.
1) This information is given for the convenience of users of this European Standard and does not constitute an endorsement by CEN of the product named. Equivalent products may be used if they can be shown to lead to the same results. SIST EN 14166:2009

Dilute 2 ml of the stock solution (4.5.1) to 20 ml with 0,1 mol/l HCl. Measure the absorbance value at 290 nm against 0,1 mol/l HCl solution (pH ≈ 1). Calculate the mass concentration, , in µg/ml of the stock solution according to equation (1): ερVMA××=w (1) where:
A is the absorbance value of the solution at 290 nm; 0 is the molar extinction coefficient in a hydrochloric acid solution of c(HCl) = 0,1 mol/l at max = 290 nm (here: 8 400 mmol-1cm-1, see [2]); Mw is the molar mass of the standard substance, in gram per mol; V is the dilution factor, i.e. 10. 4.7 Intermediate pyridoxine calibration solution, (C8H11NO3) ≈ 400 ng/ml Dilute 2 ml of pyridoxine stock solution (4.5.1) to 1000 ml with glass distilled water. Prepare on day of use. 4.8 Calibration solutions 4.8.1 Pyridoxine calibration solution 20, (C8H11NO3) = 20 ng/ml Dilute 5 ml intermediate calibration solution (4.7) to 100 ml with glass distilled water. Prepare on day of use. 4.8.2 Pyridoxine calibration solution 10, (C8H11NO3) = 10 ng/ml Dilute 25 ml of pyridoxine calibration solution 20 (4.8.1) to 50 ml with glass distilled water. Prepare on day of use. 4.8.3 Pyridoxine calibration solution 5, (C8H11NO3)
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