EN 16024:2011
(Main)Fertilizers - Determination of 1H-1,2,4-triazole in urea and in fertilizers containing urea - Method using high-performance liquid chromatography (HPLC)
Fertilizers - Determination of 1H-1,2,4-triazole in urea and in fertilizers containing urea - Method using high-performance liquid chromatography (HPLC)
This European Standard specifies a method for the determination of triazole (TZ) in urea or in fertilizers containing urea in the presence of dicyandiamide or methylpyrazole respectively using high-performance liquid chromatography (HPLC).
Düngemittel - Bestimmung von 1H-1,2,4-Triazol in Harnstoff und harnstoffhaltigen Düngemitteln - Verfahren mit Hochleistungs-Flüssigchromatographie (HPLC)
Diese Europäische Norm legt ein Verfahren zur Bestimmung von Triazol (TZ) in Harnstoff oder in harnstoffhaltigen Düngemitteln jeweils in Anwesenheit von Dicyandiamid oder Methylpyrazol mittels Hochleistungs-Flüssigchromatographie (HPLC) fest.
Engrais - Dosage du 1H-1,2,4-triazole dans l'urée et les engrais contenant de l'urée - Méthode par chromatographie liquide à haute performance (HPLC)
La présente norme décrit une méthode de détermination de la teneur en triazole (TZ) dans l’urée et dans les
engrais contenant de l’urée en présence de dicyandiamide ou de méthylpyrazole par chromatographie liquide
à haute performance (HPLC).
Gnojila - Določevanje 1H,2,4-triazola v sečnini in gnojilih, ki vsebujejo sečnino - Metoda s tekočinsko kromatografijo visoke ločljivosti (HPLC)
Ta dokument določa metodo za določevanje triazola (TZ) v sečnini ali gnojilih, ki vsebujejo sečnino, v prisotnosti diciandiamida oz. metilpirazola, z uporabo plinske kromatografije (GC). Triazol se določa s plinsko kromatografijo. Zaznava poteka prek kvadrupola. Pri analizi se uporablja zunanja kalibracijska točka. Pri kalibraciji je treba ustrezno upoštevati sečnino ali raztopino amonijevega nitrata in sečnine ter druge zaviralce nitrifikacije, npr. diciandiamid oz. metilpirazol, ki sta prisotna v njih (matrični interferenčni učinek).
General Information
- Status
- Published
- Publication Date
- 12-Apr-2011
- Withdrawal Date
- 30-Oct-2011
- Technical Committee
- CEN/TC 260 - Fertilizers and liming materials
- Drafting Committee
- CEN/TC 260/WG 7 - Chemical analysis
- Current Stage
- 9093 - Decision to confirm - Review Enquiry
- Start Date
- 08-Jul-2022
- Completion Date
- 21-Jan-2026
Relations
- Effective Date
- 28-Jan-2026
- Effective Date
- 28-Jan-2026
Overview
EN 16024:2011 (CEN) defines a validated laboratory method for the determination of 1H-1,2,4-triazole (TZ) in urea and in fertilizers containing urea using high-performance liquid chromatography (HPLC). The method addresses measurement in the presence of common conditioning agents such as dicyandiamide and 3-methylpyrazole by using reversed-phase HPLC with an electrochemical pulsed detector. It supports accurate monitoring, product quality control and regulatory compliance for fertilizer producers, testing laboratories and regulators.
Key Topics
- Scope: Determination of 1H-1,2,4-triazole in urea and urea-containing fertilizers (EN 16024:2011).
- Principle: Extraction or dissolution in water followed by reversed-phase HPLC separation and electrochemical detection.
- Calibration range: Calibration solutions covering the typical range (0.1–10 mg/l) are prepared from a 1 g/l triazole standard; calibration requires a correlation coefficient > 0.99.
- Detector & column: Uses an electrochemical pulsed detector (gold working electrode; Ag/AgCl reference) and a reversed-phase column (example: C8, 250 × 4 mm).
- Equipment verification: System suitability check using a 1 mg/l calibration solution; tolerance ±0.05 mg/l (5 % rel.).
- Sampling & sample prep: Sampling follows EN 1482-series; typical test solution prepared by dissolving ~0.2 g sample in 100 ml water and filtering through a 0.25 µm syringe filter.
Applications
The standard is practical for laboratories and manufacturers who need reliable determination of triazole residues in fertilizer matrices for purposes such as:
- Quality assurance and batch release testing of urea-based fertilizers.
- Verification of manufacturing controls where dicyandiamide or methylpyrazole are present.
- Regulatory compliance and reporting in jurisdictions that reference CEN methods.
Practical benefits include: reproducible quantification using HPLC with electrochemical detection, a clear calibration and verification procedure, and established precision data from inter-laboratory testing (round robin performed in 2009). Following EN 16024:2011 helps laboratories demonstrate method traceability and consistency.
Related Standards
- EN 1482-1 / EN 1482-2 - Sampling and sample preparation for fertilizers.
- EN 15360 - Determination of dicyandiamide by HPLC.
- EN 15905 - Determination of 3-methylpyrazole by HPLC.
- ISO 5725 series - Procedures for determining repeatability and reproducibility.
For implementation, laboratories should use reagents and apparatus specified in EN 16024:2011 (grade 3 water, acetonitrile, phosphate buffer, ultrasonic bath, electrochemical detector) and include the required test report elements such as sample identification, method reference and measurement results.
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Frequently Asked Questions
EN 16024:2011 is a standard published by the European Committee for Standardization (CEN). Its full title is "Fertilizers - Determination of 1H-1,2,4-triazole in urea and in fertilizers containing urea - Method using high-performance liquid chromatography (HPLC)". This standard covers: This European Standard specifies a method for the determination of triazole (TZ) in urea or in fertilizers containing urea in the presence of dicyandiamide or methylpyrazole respectively using high-performance liquid chromatography (HPLC).
This European Standard specifies a method for the determination of triazole (TZ) in urea or in fertilizers containing urea in the presence of dicyandiamide or methylpyrazole respectively using high-performance liquid chromatography (HPLC).
EN 16024:2011 is classified under the following ICS (International Classification for Standards) categories: 65.080 - Fertilizers. The ICS classification helps identify the subject area and facilitates finding related standards.
EN 16024:2011 has the following relationships with other standards: It is inter standard links to EN ISO 3696:1995, EN 1482-2:2024. Understanding these relationships helps ensure you are using the most current and applicable version of the standard.
EN 16024:2011 is associated with the following European legislation: EU Directives/Regulations: 2003/2003, 2019/1009; Standardization Mandates: M/335. When a standard is cited in the Official Journal of the European Union, products manufactured in conformity with it benefit from a presumption of conformity with the essential requirements of the corresponding EU directive or regulation.
EN 16024:2011 is available in PDF format for immediate download after purchase. The document can be added to your cart and obtained through the secure checkout process. Digital delivery ensures instant access to the complete standard document.
Standards Content (Sample)
2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Düngemittel - Bestimmung von 1H,2,4-Triazol in Harnstoff und in harnstoffhaltigen Düngemitteln - Verfahren mit Hochleistungs-Flüssigchromatographie (HPLC)Engrais - Dosage du 1H,2,4-triazole dans l'urée et les engrais contenant de l'urée - Méthode par chromatographie liquide à haute performance (HPLC)Fertilizers - Determination of 1H,2,4-triazole in urea and in fertilizers containing urea - Method using high-performance liquid chromatography (HPLC)65.080GnojilaFertilizersICS:Ta slovenski standard je istoveten z:EN 16024:2011SIST EN 16024:2011en,fr,de01-junij-2011SIST EN 16024:2011SLOVENSKI
STANDARD
EUROPEAN STANDARD NORME EUROPÉENNE EUROPÄISCHE NORM
EN 16024
April 2011 ICS 65.080 English Version
Fertilizers - Determination of 1H-1,2,4-triazole in urea and in fertilizers containing urea - Method using high-performance liquid chromatography (HPLC)
Engrais - Dosage du 1H-1,2,4-triazole dans l'urée et les engrais contenant de l'urée - Méthode par chromatographie liquide à haute performance (HPLC)
Düngemittel - Bestimmung von 1H-1,2,4-Triazol in Harnstoff und harnstoffhaltigen Düngemitteln - Verfahren mit Hochleistungs-Flüssigchromatographie (HPLC) This European Standard was approved by CEN on 26 February 2011.
CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.
This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions.
CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.
EUROPEAN COMMITTEE FOR STANDARDIZATION
COMITÉ EUROPÉEN DE NORMALISATION EUROPÄISCHES KOMITEE FÜR NORMUNG
Management Centre:
Avenue Marnix 17,
B-1000 Brussels © 2011 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN 16024:2011: ESIST EN 16024:2011
Statistical results of the inter-laboratory test . 10Bibliography . 11 SIST EN 16024:2011
EN ISO 3696:1995, Water for analytical laboratory use — Specification and test methods (ISO 3696:198
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