CEN/TC 454/WG 7 - Product test methods - saccharides, proteins, amino-acids

This document specifies a method for the quantitative determination of total uronic acids by High Performance Anion Exchange Chromatography coupled with Pulsed Amperometric Detection (HPAEC-PAD) after acid hydrolysis of the samples for algae and algae products. It specifies a method for the determination in one single analysis of mannuronic, glucuronic and guluronic acids in brown seaweed, and mannuronic and guluronic acids in alginate products. The sum of the individual uronic acid values is used for determining the total uronic acid content.

This document describes a method for determining the amino acid profile of algal biomass.
It specifies a method for the determination, in one single analysis, of the following amino acids: alanine, arginine, aspartic acid (combined with asparagine), cystine (dimer of cysteine, combined with cysteine), glutamic acid (combined with glutamine), glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tyrosine and valine.
This method does not apply to the determination of tryptophan. The existing draft standard ISO/DIS 4214 – Milk and milk products – Determination of amino acids in infant formula and other dairy products will be evaluated and adapted.

This document describes the application of Kjeldahl and Dumas methods for the determination of nitrogen content in algae and their relevant products.
The method was initially tested and evaluated on the algae species Nannochloropsis sp. and Palmaria palmata. The study validated this document for both algae species. This method can also be used for other algae species.