E04.03 - Light Microscopy

SIGNIFICANCE AND USE
4.1 This guide is useful for the photomicrography and photomacrography of metals and other materials.  
4.2 The subsequent processing of the photographic materials is also treated.
SCOPE
1.1 This guide outlines various methods which may be followed in the photography of metals and materials with the reflected-light microscope. Methods are included for preparation of prints and transparencies in black-and-white and in color, using both direct rapid and wet processes.  
1.2 Guidelines are suggested to yield photomicrographs of typical subjects and, to the extent possible, of atypical subjects as well. Information is included concerning techniques for the enhanced display of specific material features. Descriptive material is provided where necessary to clarify procedures. References are cited where detailed descriptions may be helpful.  
1.3 The sections appear in the following order:    
Referenced documents  
2  
Terminology  
3  
Significance and use  
4  
Magnification  
5  
Reproduction of photomicrographs  
6  
Optical systems  
7  
Illumination sources  
8  
Illumination of specimens  
9  
Focusing  
10  
Filters for photomicrography  
11  
Illumination techniques  
12  
Instant-processing films  
13  
Photographic materials  
14  
Photographic exposure  
15  
Photographic processing  
16  
Keywords  
17  
Suggestions for visual use of metallographic microscopes  
Appendix X1  
Guide for metallographic photomacrography  
Appendix X2  
Electronic photography  
Appendix X3  
1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. Specific precautionary statements are given in X1.7.  
1.5 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
4.1 These methods can be used to determine magnifications as viewed through the eyepieces of light microscopes.  
4.2 These methods can be used to calibrate microscope magnifications for photography, video systems, and projection stations.  
4.3 Reticles may be calibrated as independent articles and as components of a microscope system.
SCOPE
1.1 This guide covers methods for calculating and calibrating microscope magnifications, photographic magnifications, video monitor magnifications, grain size comparison reticles, and other measuring reticles. Reflected light microscopes are used to characterize material microstructures. Many materials engineering decisions may be based on qualitative and quantitative analyses of a microstructure. It is essential that microscope magnifications and reticle dimensions be accurate.  
1.2 The calibration using these methods is only as precise as the measuring devices used. It is recommended that the stage micrometer or scale used in the calibration should be traceable to the National Institute of Standards and Technology (NIST) or a similar organization.  
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.  
1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
4.1 This guide is useful for the photomicrography and photomacrography of metals and other materials.  
4.2 The subsequent processing of the photographic materials is also treated.
SCOPE
1.1 This guide outlines various methods which may be followed in the photography of metals and materials with the reflected-light microscope. Methods are included for preparation of prints and transparencies in black-and-white and in color, using both direct rapid and wet processes.  
1.2 Guidelines are suggested to yield photomicrographs of typical subjects and, to the extent possible, of atypical subjects as well. Information is included concerning techniques for the enhanced display of specific material features. Descriptive material is provided where necessary to clarify procedures. References are cited where detailed descriptions may be helpful.  
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory requirements prior to use. Specific precautionary statements are given in X1.7.  
1.4 The sections appear in the following order:    
Referenced documents  
2  
Terminology  
3  
Significance and use  
4  
Magnification  
5  
Reproduction of photomicrographs  
6  
Optical systems  
7  
Illumination sources  
8  
Illumination of specimens  
9  
Focusing  
10  
Filters for photomicrography  
11  
Illumination techniques  
12  
Instant-processing films  
13  
Photographic materials  
14  
Photographic exposure  
15  
Photographic processing  
16  
Keywords  
17  
Suggestions for visual use of metallographic microscopes  
Appendix X1  
Guide for metallographic photomacrography  
Appendix X2  
Electronic photography  
Appendix X3  
1.5 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

SIGNIFICANCE AND USE
4.1 These methods can be used to determine magnifications as viewed through the eyepieces of light microscopes.  
4.2 These methods can be used to calibrate microscope magnifications for photography, video systems, and projection stations.  
4.3 Reticles may be calibrated as independent articles and as components of a microscope system.
SCOPE
1.1 This guide covers methods for calculating and calibrating microscope magnifications, photographic magnifications, video monitor magnifications, grain size comparison reticles, and other measuring reticles. Reflected light microscopes are used to characterize material microstructures. Many materials engineering decisions may be based on qualitative and quantitative analyses of a microstructure. It is essential that microscope magnifications and reticle dimensions be accurate.  
1.2 The calibration using these methods is only as precise as the measuring devices used. It is recommended that the stage micrometer or scale used in the calibration should be traceable to the National Institute of Standards and Technology (NIST) or a similar organization.  
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

SIGNIFICANCE AND USE
This guide is useful for the photomicrography and photomacrography of metals and other materials.
The subsequent processing of the photographic materials is also treated.
SCOPE
1.1 This guide outlines various methods which may be followed in the photography of metals and materials with the reflected-light microscope. Methods are included for preparation of prints and transparencies in black-and-white and in color, using both direct rapid and wet processes.
1.2 Guidelines are suggested to yield photomicrographs of typical subjects and, to the extent possible, of atypical subjects as well. Information is included concerning techniques for the enhanced display of specific material features. Descriptive material is provided where necessary to clarify procedures. References are cited where detailed descriptions may be helpful.
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory requirements prior to use. Specific precautionary statements are given in X1.7.
1.4 The sections appear in the following order:

SIGNIFICANCE AND USE
These methods can be used to determine magnifications as viewed through the eyepieces of light microscopes.
These methods can be used to calibrate microscope magnifications for photography, video systems, and projection stations.
Reticles may be calibrated as independent articles and as components of a microscope system.
SCOPE
1.1 This guide covers methods for calculating and calibrating microscope magnifications, photographic magnifications, video monitor magnifications, grain size comparison reticles, and other measuring reticles. Reflected light microscopes are used to characterize material microstructures. Many materials engineering decisions may be based on qualitative and quantitative analyses of a microstructure. It is essential that microscope magnifications and reticle dimensions be accurate.
1.2 The calibration using these methods is only as precise as the measuring devices used. It is recommended that the stage micrometer or scale used in the calibration should be traceable to the National Institute of Standards and Technology (NIST) or a similar organization.
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

SIGNIFICANCE AND USE
This guide is useful for the photomicrography and photomacrography of metals and other materials.
The subsequent processing of the photographic materials is also treated.
SCOPE
1.1 This guide outlines various methods which may be followed in the photography of metals and materials with the reflected-light microscope. Methods are included for preparation of prints and transparencies in black-and-white and in color, using both direct rapid and wet processes.
1.2 Guidelines are suggested to yield photomicrographs of typical subjects and, to the extent possible, of atypical subjects as well. Information is included concerning techniques for the enhanced display of specific material features. Descriptive material is provided where necessary to clarify procedures. References are cited where detailed descriptions may be helpful.
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory requirements prior to use. Specific precautionary statements are given in X1.7.
1.4 The sections appear in the following order:Referenced documents2Terminology3Significance and use4Magnification5Reproduction of photomicrographs6Optical systems7Illumination sources8Illumination of specimens9Focusing10Filters for photomicrography11Illumination techniques12Instant-processing films13Photographic materials14Photographic exposure15Photographic processing16Keywords17Suggestions for visual use of metallographic microscopesAppendix X1Guide for metallographic phhotmacrographyAppendix X2Electronic photographyAppendix X3

SCOPE
1.1 This guide outlines various methods which may be followed in the photography of metals and materials with the reflected-light microscope. Methods are included for preparation of prints and transparencies in black-and-white and in color, using both direct rapid and wet processes.
1.2 Guidelines are suggested to yield photomicrographs of typical subjects and, to the extent possible, of atypical subjects as well. Information is included concerning techniques for the enhanced display of specific material features. Descriptive material is provided where necessary to clarify procedures. References are cited where detailed descriptions may be helpful.
1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory requirements prior to use. Specific precautionary statements are given in X1.7.Referenced documents2Terminology3Significance and use4Magnification5Reproduction of photomicrographs6Optical systems7Illumination sources8Illumination of specimens9Focusing10Filters for photomicrography11Illumination techniques12Instant-processing films13Photographic materials14Photographic exposure15Photographic processing16Keyword17Suggestions for visual use of metallographic microscopesX1Guide for metallographic phhotmacrographyX2Electronic photographyX3

SCOPE
1.1 This guide covers methods for calculating and calibrating microscope magnifications, photographic magnifications, video monitor magnifications, grain size comparison reticles, and other measuring reticles. Reflected light microscopes are used to characterize material microstructures. Many materials engineering decisions may be based on qualitative and quantitative analyses of a microstructure. It is essential that microscope magnifications and reticle dimensions be accurate.  
1.2 The calibration using these methods is only as precise as the measuring devices used. It is recommended that the stage micrometer or scale used in the calibration should be traceable to the National Institute of Standards and Technology (NIST) or a similar organization.  
1.3  This standard does not purport to address all of the safety problems, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

SCOPE
1.1 This guide covers methods for calculating and calibrating microscope magnifications, photographic magnifications, video monitor magnifications, grain size comparison reticles, and other measuring reticles. Reflected light microscopes are used to characterize material microstructures. Many materials engineering decisions may be based on qualitative and quantitative analyses of a microstructure. It is essential that microscope magnifications and reticle dimensions be accurate.  
1.2 The calibration using these methods is only as precise as the measuring devices used. It is recommended that the stage micrometer or scale used in the calibration should be traceable to the National Institute of Standards and Technology (NIST) or a similar organization.  
1.3  This standard does not purport to address all of the safety problems, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

SCOPE
1.1 This guide covers methods for calculating and calibrating microscope magnifications, photographic magnifications, video monitor magnifications, grain size comparison reticles, and other measuring reticles. Reflected light microscopes are used to characterize material microstructures. Many materials engineering decisions may be based on qualitative and quantitative analyses of a microstructure. It is essential that microscope magnifications and reticle dimensions be accurate.  
1.2 The calibration using these methods is only as precise as the measuring devices used. It is recommended that the stage micrometer or scale used in the calibration should be traceable to the National Institute of Standards and Technology (NIST) or a similar organization.  
1.3  This standard does not purport to address all of the safety problems, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

SCOPE
1.1 This guide outlines various suggested methods which may be followed in the photography of metals and materials with the reflected-light microscope. Methods are included for preparation of prints and transparencies in black-and-white and in color, using both direct rapid and wet processes.  
1.2 Descriptive material is provided where necessary to clarify procedures. References are cited where detailed descriptions may be helpful. Guidelines are suggested to yield photomicrographs of typical subjects and, to the extent possible, of atypical subjects as well. Information is included concerning techniques for the enhanced display of specific material features.
1.3 This standard does not purport to address all of the safety problems, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. Specific precautionary statements are given in Appendix X1.7.
1.4 The sections appear in the following order:  Contents Section Magnification 4 Reproduction of photomicrographs 5 Optical systems 6 Illumination sources 7 Illumination of specimens 8 Focusing 9 Filters for photomicrography 10 Illumination techniques 11 Photographic materials 12 Photographic exposure 13 Photographic processing 14 Suggestions for visual use of metallographic microscopes X1 Guide for metallographic photomacrography X2 Video image printing X3