SIST EN 12780:2003

2003-01.Slovenski inštitut za standardizacijo. Razmnoževanje celote ali delov tega standarda ni dovoljeno.Kakovost vode - Ugotavljanje prisotnosti in števila Pseudomonas aeruginosa z membransko filtracijoWasserbeschaffenheit - Nachweis und Zählung von Pseudomonas aeruginosa durch MembranfiltrationQualité de l'eau - Détection et dénombrement de Pseudomonas aeruginosa par filtration sur membraneWater quality - Detection and enumeration of Pseudomonas aeruginosa by membrane filtration13.060.70Preiskava bioloških lastnosti vodeExamination of biological properties of waterICS:Ta slovenski standard je istoveten z:EN 12780:2002SIST EN 12780:2003en01-januar-2003SIST EN 12780:2003SLOVENSKI
STANDARD
EUROPEAN STANDARDNORME EUROPÉENNEEUROPÄISCHE NORMEN 12780May 2002ICS 07.100.20English versionWater quality - Detection and enumeration of Pseudomonasaeruginosa by membrane filtrationQualité de l'eau - Détection et dénombrement dePseudomonas aeruginosa par filtration sur membraneWasserbeschaffenheit - Nachweis und Zählung vonPseudomonas aeruginosa durch MembranfiltrationThis European Standard was approved by CEN on 17 February 2002.CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this EuropeanStandard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such nationalstandards may be obtained on application to the Management Centre or to any CEN member.This European Standard exists in three official versions (English, French, German). A version in any other language made by translationunder the responsibility of a CEN member into its own language and notified to the Management Centre has the same status as the officialversions.CEN members are the national standards bodies of Austria, Belgium, Czech Republic, Denmark, Finland, France, Germany, Greece,Iceland, Ireland, Italy, Luxembourg, Malta, Netherlands, Norway, Portugal, Spain, Sweden, Switzerland and United Kingdom.EUROPEAN COMMITTEE FOR STANDARDIZATIONCOMITÉ EUROPÉEN DE NORMALISATIONEUROPÄISCHES KOMITEE FÜR NORMUNGManagement Centre: rue de Stassart, 36
B-1050 Brussels© 2002 CENAll rights of exploitation in any form and by any means reservedworldwide for CEN national Members.Ref. No. EN 12780:2002 ESIST EN 12780:2003

Further information about Pseudomonas aeruginosa.13Annex B (informative)
Alternative media.14Bibliography.15SIST EN 12780:2003

examination — Part 1: General rules for the
preparation of the initial suspensionand decimal dilutions.ISO 8199, Water quality — General guide to the enumeration of micro-organisms by culture.SIST EN 12780:2003

HPO4)1,5 gMagnesium sulfate heptahydrate (MgSO4
7H2O)1,5 gAgar15,0 gWater (distilled or equivalent)1 000 ml5.3.1.2 PreparationDissolve the ingredients in the water by heating. Cool down to (45 to 50) °C and adjust the pH corresponding to7,2 ± 0,2 at 25 °C, using either hydrochloric acid or sodium hydroxide. Dispense the medium in 5 ml aliquots intoculture tubes which are capped and autoclaved at (121 ± 3) °C for 15 min. Allow the tubes to cool and solidify inslants.Store in the dark at (5 ± 3) °C and use within 3 months.5.3.2 Acetamide broth5.3.2.1 CompositionSolution APotassium di-hydrogenphosphate (KH2PO4)1,0 gMagnesium sulfate anhydrous (MgSO4)0,2 gAcetamide2,0 gSodium Chloride (NaCl)0,2 gWater (distilled or equivalent, ammonia free)900 mlDissolve the ingredients in water and then adjust the pH to correspond to 7,0 ± 0,5 at 25 °C with either hydrochloricacid or sodium hydroxide.CAUTION — Acetamide is carcinogenic and irritant - appropriate precautions shall be taken when weighingout, preparing and discarding the medium.SIST EN 12780:2003
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