General Information

Abstract

This document specifies the evaluation procedure of fine bubble elimination for fine bubble dispersion in water. This document is applicable only to fine bubbles without shell.

Status
Not Published
Current Stage
5020 - FDIS ballot initiated: 2 months. Proof sent to secretariat
Start Date
13-Aug-2026
Completion Date
13-Aug-2026

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ISO/FDIS 24261-1 - Fine bubble technology — Elimination method for sample characterization — Part 1: Evaluation procedure

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Overview

ISO/FDIS 24261-1:2026 defines a standardized evaluation procedure for the elimination of fine bubbles from aqueous dispersions, focusing exclusively on fine bubbles without shells. Fine bubble technology is increasingly utilized in sectors such as water treatment, cleaning, agriculture, aquaculture, and biomedical fields. Accurate sample characterization is critical for assessing the quality and performance of fine bubble solutions. This international standard, developed by ISO Technical Committee 281 (Fine bubble technology), supports the comparison and optimization of elimination techniques for fine bubble dispersions in water.

Key Topics

  • Scope and Applicability:
    • Specifies evaluation methods for eliminating fine bubbles from water-based dispersions
    • Applicable solely to fine bubbles without shell
  • Sample Preparation:
    • Requires generation of fine bubble dispersion using purified water and gases (e.g., air, nitrogen, oxygen)
    • Advises against use of stabilizing agents or contaminants that interfere with measurement accuracy
  • Measurement Criteria:
    • Outlines confirmation of measurement instruments’ ability to detect total concentration and particle size ranges
    • Recommends suitable analytical techniques, such as particle tracking analysis and laser diffraction, to measure both the number and volume concentration indices
  • Environmental Conditions:
    • Emphasizes the importance of air cleanliness (e.g., ISO Class 7, ISO 14644-1) and controlled temperature/pressure to ensure measurement precision
  • Elimination Efficiency Evaluation:
    • Defines calculation of number reduction rate and volume reduction rate before and after the elimination process
    • Stresses analysis of both indices to distinguish genuine fine bubble elimination from particle aggregation
  • Reporting Requirements:
    • Outlines comprehensive reporting on elimination efficiency, measurement methods, sample details, and environmental conditions to ensure traceability and reproducibility

Applications

Standardized fine bubble elimination evaluation brings measurable benefits across various industries where water purity and precise bubble control are critical:

  • Water Treatment: Optimize the efficiency of fine bubble removal for processes such as dissolved air flotation or wastewater purification.
  • Industrial Cleaning: Assess and improve the performance of cleaning systems using fine bubble technology, ensuring consistency and reliability.
  • Agriculture and Aquaculture: Monitor water quality and aeration efficiency in irrigation and aquatic animal husbandry, where dissolved gases and microbubbles affect yields.
  • Biomedical Research: Enable accurate sample preparation and particle separation in laboratory settings, supporting repeatable experimental results.
  • Technology Development: Provide manufacturers of fine bubble generators and related equipment with clear benchmarks for product validation and quality control.

Related Standards

Several key international standards complement ISO/FDIS 24261-1 by providing frameworks for terminology, sampling, measurement, and environmental control:

  • ISO 20480-1 - Fine bubble technology - General principles for usage and measurement - Part 1: Terminology
  • ISO 3696 - Water for analytical laboratory use - Specification and test methods
  • ISO 14644-1 - Cleanrooms and associated controlled environments - Classification of air cleanliness by particle concentration
  • ISO 20298-1 - Fine bubble technology - Sampling and sample preparation for measurement - Part 1: Ultrafine bubble dispersion in water
  • ISO 20480-2 - Fine bubble technology - Categorization of the attributes of fine bubbles
  • ISO 21910-1 - Fine bubble technology - Characterization of microbubbles
  • ISO/TR 23015 - Fine bubble technology - Measurement technique matrix for the characterization of fine bubbles

By adhering to ISO/FDIS 24261-1 and these relevant standards, organizations can ensure accurate, repeatable, and internationally recognized sample characterization and fine bubble elimination processes.

Relations

Effective Date
30-May-2026

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ISO/FDIS 24261-1 - Fine bubble technology — Elimination method for sample characterization — Part 1: Evaluation procedure

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Frequently Asked Questions

ISO/FDIS 24261-1 is a draft published by the International Organization for Standardization (ISO). Its full title is "Fine bubble technology — Elimination method for sample characterization — Part 1: Evaluation procedure". This standard covers: This document specifies the evaluation procedure of fine bubble elimination for fine bubble dispersion in water. This document is applicable only to fine bubbles without shell.

This document specifies the evaluation procedure of fine bubble elimination for fine bubble dispersion in water. This document is applicable only to fine bubbles without shell.

ISO/FDIS 24261-1 is classified under the following ICS (International Classification for Standards) categories: 13.060.60 - Examination of physical properties of water. The ICS classification helps identify the subject area and facilitates finding related standards.

ISO/FDIS 24261-1 has the following relationships with other standards: It is inter standard links to ISO 24261-1:2020. Understanding these relationships helps ensure you are using the most current and applicable version of the standard.

ISO/FDIS 24261-1 is available in PDF format for immediate download after purchase. The document can be added to your cart and obtained through the secure checkout process. Digital delivery ensures instant access to the complete standard document.

Standards Content (Sample)


FINAL DRAFT
International
Standard
ISO/TC 281
Fine bubble technology —
Secretariat: JISC
Elimination method for sample
Voting begins on:
characterization —
2026-08-13
Part 1:
Voting terminates on:
2026-10-08
Evaluation procedure
Technologie des fines bulles — Méthode d'élimination pour la
caractérisation de l'échantillon —
Partie 1: Mode opératoire d'évaluation
RECIPIENTS OF THIS DRAFT ARE INVITED TO SUBMIT,
WITH THEIR COMMENTS, NOTIFICATION OF ANY
RELEVANT PATENT RIGHTS OF WHICH THEY ARE AWARE
AND TO PROVIDE SUPPOR TING DOCUMENTATION.
IN ADDITION TO THEIR EVALUATION AS
BEING ACCEPTABLE FOR INDUSTRIAL, TECHNO-
LOGICAL, COMMERCIAL AND USER PURPOSES, DRAFT
INTERNATIONAL STANDARDS MAY ON OCCASION HAVE
TO BE CONSIDERED IN THE LIGHT OF THEIR POTENTIAL
TO BECOME STAN DARDS TO WHICH REFERENCE MAY BE
MADE IN NATIONAL REGULATIONS.
Reference number
FINAL DRAFT
International
Standard
ISO/TC 281
Fine bubble technology —
Secretariat: JISC
Elimination method for sample
Voting begins on:
characterization —
Part 1:
Voting terminates on:
Evaluation procedure
Technologie des fines bulles — Méthode d'élimination pour la
caractérisation de l'échantillon —
Partie 1: Mode opératoire d'évaluation
RECIPIENTS OF THIS DRAFT ARE INVITED TO SUBMIT,
WITH THEIR COMMENTS, NOTIFICATION OF ANY
RELEVANT PATENT RIGHTS OF WHICH THEY ARE AWARE
AND TO PROVIDE SUPPOR TING DOCUMENTATION.
© ISO 2026
IN ADDITION TO THEIR EVALUATION AS
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may
BEING ACCEPTABLE FOR INDUSTRIAL, TECHNO-
LOGICAL, COMMERCIAL AND USER PURPOSES, DRAFT
be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on
INTERNATIONAL STANDARDS MAY ON OCCASION HAVE
the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below
TO BE CONSIDERED IN THE LIGHT OF THEIR POTENTIAL
or ISO’s member body in the country of the requester.
TO BECOME STAN DARDS TO WHICH REFERENCE MAY BE
MADE IN NATIONAL REGULATIONS.
ISO copyright office
CP 401 • Ch. de Blandonnet 8
CH-1214 Vernier, Geneva
Phone: +41 22 749 01 11
Email: copyright@iso.org
Website: www.iso.org
Published in Switzerland Reference number
ii
Contents Page
Foreword .iv
Introduction .v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Requirements . 1
4.1 Sample .1
4.2 Measuring instruments .2
5 Environment . 2
6 Evaluation of fine bubble elimination efficiency . 2
6.1 General .2
6.2 Reduction rate .2
6.3 Confirmation of contaminants aggregation .3
6.4 Evaluation procedure of fine bubble elimination .3
7 Test report . 4
7.1 Report of the testing results.4
7.2 Report of the testing conditions .4
Annex A (informative) Simulations of fine bubble elimination process . 5
Annex B (informative) Decrease of concentration caused by fine bubble elimination and
aggregation . 7
Bibliography . 9

iii
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out through
ISO technical committees. Each member body interested in a subject for which a technical committee
has been established has the right to be represented on that committee. International organizations,
governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely
with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are described
in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the different types
of ISO documents should be noted. This document was drafted in accordance with the editorial rules of the
ISO/IEC Directives, Part 2 (see www.iso.org/directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of any patent
rights identified during the development of the document will be in the Introduction and/or on the ISO list of
patent declarations received (see www.iso.org/patents).
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation of the voluntary nature of standards, the meaning of ISO specific terms and expressions
related to conformity assessment, as well as information about ISO's adherence to the World Trade
Organization (WTO) principles in the Technical Barriers to Trade (TBT), see www.iso.org/iso/foreword.html.
This document was prepared by Technical Committee ISO/TC 281 Fine bubble technology.
This second edition cancels and replaces the first edition (ISO 24261-1:2020), of which it constitutes a minor
revision.
The changes are as follows:
— in 7.1, the following list item was added "f)  Number of this document";
— in Figure A.1 and Figure A.2, the unit "million/ml" was revised to "×10⁶ particles/ml".
A list of all parts in the ISO 24261 series can be found on the ISO website.
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www.iso.org/members.html.

iv
Introduction
Fine bubble technology has recently seen growth in its application to markets in cleaning, water treatment,
agriculture, and aquaculture as well as biomedical fields. Now, methods are required to evaluate the
generation systems. Especially characteristics like the number concentration index and the size index of fine
bubbles are indispensable for those evaluations.
Furthermore, fine bubble dispersion water may contain other solid and liquid particles. Clearly due to this
is a concern, as it may be impossible to evaluate the characteristics of fine bubbles. Therefore, it is an urgent
task to address this concern.
There are several measurement methods widely used to evaluate the number concentration index and the
size index of particles. However, there are few methods to distinguish bubbles in fine bubble dispersions
from other particles.
This issue can be resolved, using the phenomenon by which the bubbles can be eliminated without any
residues after dissolution and flotation. If a method that eliminates fine bubbles in specific size range is
known, it is possible to distinguish fine bubbles from other solid and liquid particles. The eliminated
particles can be fine bubbles. If most of fine bubbles decreased, a solution that doesn’t have them can be used
as a blank solution for measurements as background. Because it is expected that fine bubbles elimination
techniques will develop further, standardizing elimination techniques and evaluation method is required.
This document is intended to specify the evaluation method for elimination efficiency of fine bubbles from
fine bubble dispersions in water.
Standardization for evaluating elimination efficiency of fine bubbles enables easy and clear comparison
among the several elimination techniques and realizes the optimization of conditions for respective
elimination techniques.
v
FINAL DRAFT International Standard ISO/FDIS 24261-1:2026(en)
Fine bubble technology — Elimination method for sample
characterization —
Part 1:
Evaluation procedure
1 Scope
This document specifies the evaluation procedure of fine bubble elimination for fine bubble dispersion in
water. This document is applicable only to fine bubbles without shell.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content constitutes
requirements of this document. For dated references, only the edition cited applies. For undated references,
the latest edition of the referenced document (including any amendments) applies.
ISO 20480-1, Fine bubble technology — General principles for usage and measurement of fine bubbles — Part 1:
Terminology
3 Terms and definitions
For the purposes of this document, the terms and definitions given in ISO 20480-1 and the following apply.
ISO and IEC maintain terminology databases for use in standardization at the following addresses:
— ISO Online browsing platform: available at https:// www .iso .org/ obp
— IEC Electropedia: available at https:// www .electropedia .org/
3.1
elimination of fine bubbles
process for the decrease of concentration index of fine bubbles
3.2
fine bubble dispersion
FBD
liquid which contains fine bubbles
[SOURCE: ISO 20298-1:2018, 3.1]
4 Requirements
4.1 Sample
The fine bubble dispersion to be evaluated shall be generated by cleaned fine bubble generating systems
using pure water and pure gas such as air, nitrogen and oxygen.
The purity level of water and gas depends on the sample whose size and concentration indices of fine
bubbles should be evaluated because fine bubble elimination is one of the evaluating process for specified

samples. So, the purity level cannot be generally determined as measurement condition for evaluations of
the elimination efficiency.
The fine bubble dispersion shall not contain stabilizing agents.
If the measurement accuracy of size and concentration indices is very important (e.g. for accreditation
purposes), a water purity level of Grade 1 according to ISO 3696 is recommended for the water used fo
...


Date: 2026-06-05
ISO /TC 281
Secretariat: JISC
Date: 2026-xx
Fine bubble technology — Elimination method for sample
characterization —
—
Part 1:
Evaluation procedure
Technologie des fines bulles — Méthode d'élimination pour la caractérisation de l'échantillon — —
Partie 1: Mode opératoire d'évaluation
FDIS stage
All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication
may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying,
or posting on the internet or an intranet, without prior written permission. Permission can be requested from either ISO
at the address below or ISO’s member body in the country of the requester.
ISO copyright office
CP 401 • Ch. de Blandonnet 8 • CP 401
CH-1214 Vernier, Geneva, Switzerland
Tel.Phone: + 41 22 749 01 11
Fax + 41 22 749 09 47
E-mail: copyright@iso.org
Website: www.iso.org
Published in Switzerland
ii
Contents
Foreword . iv
Introduction . v
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Requirements . 1
4.1 Sample . 1
4.2 Measuring instruments . 2
5 Environment . 2
6 Evaluation of fine bubble elimination efficiency . 2
6.1 General . 2
6.2 Reduction rate . 2
6.3 Confirmation of contaminants aggregation . 3
6.4 Evaluation procedure of fine bubble elimination . 3
7 Test report . 4
7.1 Report of the testing results. 4
7.2 Report of the testing conditions . 4
Annex A (informative) Simulations of fine bubble elimination process . 6
Annex B (informative) Decrease of concentration caused by fine bubble elimination and
aggregation . 8
Bibliography . 10

iii
Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
bodies (ISO member bodies). The work of preparing International Standards is normally carried out through
ISO technical committees. Each member body interested in a subject for which a technical committee has been
established has the right to be represented on that committee. International organizations, governmental and
non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the
International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.
The procedures used to develop this document and those intended for its further maintenance are described
in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the different types of
ISO documents should be noted. This document was drafted in accordance with the editorial rules of the
ISO/IEC Directives, Part 2 (see www.iso.org/directives).
Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of any patent rights
identified during the development of the document will be in the Introduction and/or on the ISO list of patent
declarations received (see www.iso.org/patents).
Any trade name used in this document is information given for the convenience of users and does not
constitute an endorsement.
For an explanation of the voluntary nature of standards, the meaning of ISO specific terms and expressions
related to conformity assessment, as well as information about ISO's adherence to the World Trade
Organization (WTO) principles in the Technical Barriers to Trade (TBT), see www.iso.org/iso/foreword.html.
This document was prepared by Technical Committee ISO/TC 281 Fine bubble technology.
This second edition cancels and replaces the first edition (ISO 24261-1:2020), of which it constitutes a minor
revision.
The changes are as follows:
— in 7.1, the following list item was added "f)  Number of this document";
— in Figure A.1 and Figure A.2, the unit "million/ml" was revised to "×10⁶ particles/ml".
A list of all parts in the ISO 24261 series can be found on the ISO website.
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www.iso.org/members.html.
iv
Introduction
Fine bubble technology has recently seen growth in its application to markets in cleaning, water treatment,
agriculture, and aquaculture as well as biomedical fields. Now, methods are required to evaluate the
generation systems. Especially characteristics like the number concentration index and the size index of fine
bubbles are indispensable for those evaluations.
Furthermore, fine bubble dispersion water may contain other solid and liquid particles. Clearly due to this is
a concern, as it may be impossible to evaluate the characteristics of fine bubbles. Therefore, it is an urgent task
to address this concern.
There are several measurement methods widely used to evaluate the number concentration index and the
size index of particles. However, there are few methods to distinguish bubbles in fine bubble dispersions from
other particles.
This issue can be resolved, using the phenomenon by which the bubbles can be eliminated without any
residues after dissolution and flotation. If a method that eliminates fine bubbles in specific size range is known,
it is possible to distinguish fine bubbles from other solid and liquid particles. The eliminated particles can be
fine bubbles. If most of fine bubbles decreased, a solution that doesn’t have them can be used as a blank
solution for measurements as background. Because it is expected that fine bubbles elimination techniques will
develop further, standardizing elimination techniques and evaluation method is required.
This document is intended to specify the evaluation method for elimination efficiency of fine bubbles from fine
bubble dispersions in water.
Standardization for evaluating elimination efficiency of fine bubbles enables easy and clear comparison among
the several elimination techniques and realizes the optimization of conditions for respective elimination
techniques.
v
Fine bubble technology — Elimination method for sample
characterization — —
Part 1:
Evaluation procedure
1 Scope
This document specifies the evaluation procedure of fine bubble elimination for fine bubble dispersion in
water. This document is applicable only to fine bubbles without shell.
2 Normative references
The following documents are referred to in the text in such a way that some or all of their content constitutes
requirements of this document. For dated references, only the edition cited applies. For undated references,
the latest edition of the referenced document (including any amendments) applies.
ISO 20480--1, Fine bubble technology — General principles for usage and measurement of fine bubbles — Part
1: Terminology
3 Terms and definitions
For the purposes of this document, the terms and definitions given in ISO 20480-1 and the following apply.
ISO and IEC maintain terminologicalterminology databases for use in standardization at the following
addresses:
— — ISO Online browsing platform: available at https://www.iso.org/obp
— — IEC Electropedia: available at https://www.electropedia.org/
3.1 3.1
elimination of fine bubbles
process for the decrease of the concentration index of fine bubbles
3.2 3.2
fine bubble dispersion
FBD
liquid which contains fine bubbles
[SOURCE: ISO 20298-1:2018, 3.1]
4 Requirements
4.1 Sample
The fine bubble dispersion to be evaluated shall be generated by cleaned fine bubble generating systems using
pure water and pure gas such as air, nitrogen and oxygen.
The purity level of water and gas depends on the sample whose size and concentration indices of fine bubbles
should be evaluated because fine bubble elimination is one of the evaluating process for specified samples. So,
the purity level cannot be generally determined as measurement condition for evaluations of the elimination
efficiency.
The fine bubble dispersion shall not contain stabilizing agents.
If the measurement accuracy of size and concentration indices is very important (e.g. for accreditation
purposes), a water purity level of Grade 1 according to ISO 3696 is recommended for the water used for
generation of FBD.
4.2 Measuring instruments
When the measuring instruments are selected for evaluating fine bubble elimination for FBD in water the
following parameters about concentration and size range shall be confirmed, which depends on the
characteristics of the sample to be evaluated.:
a) a) Total number concentration and/or total volume concentration including fine bubbles and
contaminants such as solid particles and liquid particles can be measured. Water diluent can be used for
diluting the FBD in water when the concentration is over the measuring range.
b) b) Total size range including fine bubbles, contaminants and aggregates of contaminants can be
measured. Another measuring instrument can be used to confirm the larger aggregates.
EXAMPLE Particle tracking analysis method can be used for evaluation of number concentration, and laser
diffraction method can be used for evaluation of volume concentration. ISO/TR 23015 can be referred to for details of
measurement techniques which can be used for FBD in water.
5 Environment
The classification of air cleanliness should be applied for the measurements to prevent any contamination by
impurities. Ambient temperature and atmospheric pressure should be stable to keep the stability of ultrafine
bubbles.
Air cleanliness, ambient temperature and atmospheric pressure depend on the environment to evaluating size
and concentration indices of specified samples because fine bubble elimination is one of the evaluating process
for specified samples. Therefore, the environment can
...