IWA 32:2019

INTERNATIONAL IWA
WORKSHOP 32
AGREEMENT
First edition
2019-04
Screening of genetically modified
organisms (GMOs) in cotton and
textiles
Criblage pour la détection des organismes génétiquement modifiés
(OGM) dans le coton et les textiles
Reference number
©
ISO 2019
© ISO 2019
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ii © ISO 2019 – All rights reserved

Contents Page
Foreword .v
Introduction .vi
1 Scope . 1
2 Normative references . 1
3 Terms and definitions . 1
4 Principle . 2
5 Sample preparation . 2
6 DNA isolation . 3
6.1 General . 3
6.2 Principle . 3
6.3 Chemicals, reagents and equipment. 4
6.3.1 Reagents . 4
6.3.2 Apparatus and equipment . 4
6.4 Procedure . 5
6.4.1 General. 5
6.4.2 Protocol . 5
6.5 Results . 6
6.5.1 Analysis . 6
7 DNA quality control . 6
7.1 General . 6
7.2 Principle . 6
7.3 Chemicals, reagents and equipment, including reference materials. 6
7.3.1 Reagents . 6
7.3.2 Apparatus and equipment . 7
7.4 Procedure . 8
7.4.1 General. 8
7.4.2 Safety precautions . 8
7.4.3 Pre-treatment . 8
7.4.4 Amount of sample . 8
7.4.5 Procedure . 8
7.5 Results . 8
7.5.1 Calculations . 8
7.5.2 Interpretation and expression of results . 8
7.5.3 Results . 8
8 GM element screening. 8
8.1 Principle . 8
8.2 Chemicals, reagents and equipment, including reference materials. 9
8.2.1 Reagents and materials . 9
8.2.2 Apparatus and equipment .10
8.3 Procedure .10
8.3.1 General.10
8.3.2 Safety precautions .10
8.3.3 Pre-treatment .10
8.3.4 Amount of sample .10
8.3.5 Procedure .10
8.4 Interpretation and expression of results .10
8.5 Results .11
8.6 Reporting of data collection.11
9 Test report .11
Annex A (informative) Overview of known GM cotton events .12
Annex B (informative) Overview of detection methods applied by RIKILT .16
Annex C (informative) In-house validation RIKILT .18
Annex D (informative) Workshop contributors .27
Bibliography .30
iv © ISO 2019 – All rights reserved

Foreword
ISO (the International Organization for Standardization) is a worldwide federation of national standards
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electrotechnical standardization.
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described in the ISO/IEC Directives, Part 1. In particular, the different approval criteria needed for the
different types of ISO documents should be noted. This document was drafted in accordance with the
editorial rules of the ISO/IEC Directives, Part 2 (see www .iso .org/directives).
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expressions related to conformity assessment, as well as information about ISO's adherence to the
World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT) see www .iso
.org/iso/foreword .html.
International Workshop Agreement IWA 32 was approved at a workshop hosted by the Netherlands
Standardization Institute (NEN), in association with the Organic Cotton Accelerator, held in New Delhi,
India, in January 2019.
Any feedback or questions on this document should be directed to the user’s national standards body. A
complete listing of these bodies can be found at www .iso .org/members .html.
Introduction
0.1  General
This purpose of this document is to provide guidance to laboratories worldwide to assess, in a
standardized way, whether cotton, cotton fibre and/or cotton-derived materials are produced from, or
contain materials from, genetically modified (GM) cotton plants. This document is intended for non-GM
cotton and textiles production lines, but it can be applied to any production line that wants to check the
presence of GM cotton.
0.2  Protocol
The GM screening protocol described in this document is based on Polymerase Chain Reaction (PCR)-
based methods, as these methods are the minimal set of DNA-based methods to cover all know
...